Journal of Bioscience and Agriculture Research
Volume 36 - Issue 01 | Year of Publication: 2026
Article information: Research Article | No. 336, 2026 | Country: Bangladesh | pp. 2826-2834 | Open Access
Title: Comparative performance of okra varieties under open field and net tunnel conditions
Authors: A.F.M. Jamal Uddin, Habibur Rahman Remel, Naisha Najiat, Sushoma Showkat Shanta and Tamima Dastagir
DOI: https://doi.org/10.18801/jbar.360126.336
Title: Comparative performance of okra varieties under open field and net tunnel conditions
Authors: A.F.M. Jamal Uddin, Habibur Rahman Remel, Naisha Najiat, Sushoma Showkat Shanta and Tamima Dastagir
DOI: https://doi.org/10.18801/jbar.360126.336
Comparative performance of okra varieties under open field and net tunnel conditions
Abstract
The experiment was conducted at the Horticultural Farm of Sher-e-Bangla Agricultural University, Dhaka, Bangladesh, from April to September 2025, to evaluate the performance of okra (Abelmoschus esculentus L.) varieties under net tunnel and open-field conditions. The study was laid out in a two-factor factorial randomized complete block design (RCBD) with four replications, comprising two growing environments (net tunnel and open field) and four genotypes (Marvelous, Labonyo, Moushumi, and a local variety). Results revealed significant variations in plant growth and yield attributes due to the interaction between environment and genotype. Cultivation under net tunnel conditions improved the germination percentage, accelerated flowering, and enhanced pod setting compared to open-field conditions. Among the tested genotypes, ‘Marvelous’ produced the tallest plants (118.8 cm), while G4 (Moushumi) demonstrated the highest productivity, recording the greatest number of pods per plant (26.2) and the maximum yield (64.7 t ha⁻¹). The average yield under net tunnel cultivation (59.0 t ha⁻¹) was higher than that under open field (22.9 t ha⁻¹). The findings indicate that combining net tunnel technology with the high-yielding, heat-tolerant okra variety ‘Moushumi’ offers a promising strategy to reduce heat stress and significantly improve okra productivity under Bangladeshi conditions.
Keywords: Okra (Abelmoschus esculentus L.); Net tunnel; Open field; Genotype × environment interaction; Yield performance; Heat stress; Protected cultivation
Abstract
The experiment was conducted at the Horticultural Farm of Sher-e-Bangla Agricultural University, Dhaka, Bangladesh, from April to September 2025, to evaluate the performance of okra (Abelmoschus esculentus L.) varieties under net tunnel and open-field conditions. The study was laid out in a two-factor factorial randomized complete block design (RCBD) with four replications, comprising two growing environments (net tunnel and open field) and four genotypes (Marvelous, Labonyo, Moushumi, and a local variety). Results revealed significant variations in plant growth and yield attributes due to the interaction between environment and genotype. Cultivation under net tunnel conditions improved the germination percentage, accelerated flowering, and enhanced pod setting compared to open-field conditions. Among the tested genotypes, ‘Marvelous’ produced the tallest plants (118.8 cm), while G4 (Moushumi) demonstrated the highest productivity, recording the greatest number of pods per plant (26.2) and the maximum yield (64.7 t ha⁻¹). The average yield under net tunnel cultivation (59.0 t ha⁻¹) was higher than that under open field (22.9 t ha⁻¹). The findings indicate that combining net tunnel technology with the high-yielding, heat-tolerant okra variety ‘Moushumi’ offers a promising strategy to reduce heat stress and significantly improve okra productivity under Bangladeshi conditions.
Keywords: Okra (Abelmoschus esculentus L.); Net tunnel; Open field; Genotype × environment interaction; Yield performance; Heat stress; Protected cultivation
Article information: Research Article | No. 337, 2026 | Country: Bangladesh | pp. 2836-2844 | Open Access
Title: Influence of Explants and Sterilants on In-Vitro Regeneration of Gerbera (Gerbera jamesonii)
Authors: Ireen Nahar, Md Shafikur Rahman, Md. Mainul Islam Rashad, Md. Rafiqul Islam and Khadiza Khatun
DOI: https://doi.org/10.18801/jbar.360126.337
Title: Influence of Explants and Sterilants on In-Vitro Regeneration of Gerbera (Gerbera jamesonii)
Authors: Ireen Nahar, Md Shafikur Rahman, Md. Mainul Islam Rashad, Md. Rafiqul Islam and Khadiza Khatun
DOI: https://doi.org/10.18801/jbar.360126.337
Influence of Explants and Sterilants on In-Vitro Regeneration of Gerbera (Gerbera jamesonii)
Abstract
Efficient micropropagation of Gerbera jamesonii is essential for large-scale production. Usually, multiplication is accomplished using rhizome cuttings or seeds. Using both direct and indirect tissue culture techniques has resulted in fast multiplication of gerbera cultivars with enhanced agronomic features. To optimize propagation efficiency, we evaluated the effect of explant type, size, and hormonal combinations on in vitro callus formation across three Gerbera varieties (red, yellow, and pink). Specifically, leaf and flower bud were cultured on Murashige and Skoog medium supplemented with varying concentrations of BAP (2.0-5.0 mg L-¹) and NAA (0.1-1.0 mg L-¹). It is worth noting that the red variety exhibited better response in callus induction than the yellow and pink varieties. Among the tested tissues, good response was observed in the flower buds measuring 0.6-1.0 cm (90%), or the 3rd-position leaf (80% callus induction). In addition, to find a suitable protocol for minimizing microbial contamination, we customized the sterilization process in different way. For leaf explants, a dual HgCl₂ treatment combined with Tween-20 produced a 75% callus response alongside minimal contamination. In contrast, flower bud explants responded best to a sequential protocol involving Bavistin, ethanol, and Clorox, which achieved an 85% induction rate. Further In-Vitro regeneration studies of gerbera are necessary to develop a protocol for large-scale propagation of gerbera in Bangladesh.
Key Words: Explants, Sterilizing agents, Micropropagation and Gerbera jamesonii
Abstract
Efficient micropropagation of Gerbera jamesonii is essential for large-scale production. Usually, multiplication is accomplished using rhizome cuttings or seeds. Using both direct and indirect tissue culture techniques has resulted in fast multiplication of gerbera cultivars with enhanced agronomic features. To optimize propagation efficiency, we evaluated the effect of explant type, size, and hormonal combinations on in vitro callus formation across three Gerbera varieties (red, yellow, and pink). Specifically, leaf and flower bud were cultured on Murashige and Skoog medium supplemented with varying concentrations of BAP (2.0-5.0 mg L-¹) and NAA (0.1-1.0 mg L-¹). It is worth noting that the red variety exhibited better response in callus induction than the yellow and pink varieties. Among the tested tissues, good response was observed in the flower buds measuring 0.6-1.0 cm (90%), or the 3rd-position leaf (80% callus induction). In addition, to find a suitable protocol for minimizing microbial contamination, we customized the sterilization process in different way. For leaf explants, a dual HgCl₂ treatment combined with Tween-20 produced a 75% callus response alongside minimal contamination. In contrast, flower bud explants responded best to a sequential protocol involving Bavistin, ethanol, and Clorox, which achieved an 85% induction rate. Further In-Vitro regeneration studies of gerbera are necessary to develop a protocol for large-scale propagation of gerbera in Bangladesh.
Key Words: Explants, Sterilizing agents, Micropropagation and Gerbera jamesonii
Volume 36 - Issue 02 | Year of Publication: 2026
Article information: Research Article | No. 338, 2026 | Country: Bangladesh | pp. 2845-2853 | Open Access
Title: Interactive effects of nitrogen dose and split application schedule on growth and floral yield of Lisianthus (Eustoma grandiflorum)
Authors: A.F.M Jamal Uddin, Paing Mra Ching, Tamima Dastagir, Tanmim Akter Mim and Naisha Najiat
DOI: https://doi.org/10.18801/jbar.360226.338
Title: Interactive effects of nitrogen dose and split application schedule on growth and floral yield of Lisianthus (Eustoma grandiflorum)
Authors: A.F.M Jamal Uddin, Paing Mra Ching, Tamima Dastagir, Tanmim Akter Mim and Naisha Najiat
DOI: https://doi.org/10.18801/jbar.360226.338
Interactive effects of nitrogen dose and split application schedule on growth and floral yield of Lisianthus (Eustoma grandiflorum)
Abstract
A field experiment was conducted at the Horticulture Farm, Sher-e-Bangla Agricultural University, Dhaka, during the period from September 2025 to January 2026 to study the effect of nitrogen doses and split application schedule on growth and floral yield of Lisianthus (variety, SAU Purple Nandini). Three nitrogen doses, namely N₁ = 112 kg ha⁻¹ (BARI recommended dose for marigold), N₂ = 150 kg ha⁻¹, and N₃ = 188 kg ha⁻¹, along with three split application treatments: S₁ (full dose applied as basal), S₂ (two splits: 50% basal + 50% at 30 days after transplanting), and S₃ (three splits: 33% basal + 33% at 30 DAT + 34% at 60 DAT), were evaluated in a Randomized Complete Block Design (RCBD) with three replications. Data on different growth, flower yield, and quality attributes were recorded, and all treatments showed significant variations. Among nitrogen doses, the maximum number of flower buds/plant (14.1), flower diameter (7.6 cm), and flower stalk/ plant (4.45) were found in the N3 treatment. Among the split application maximum number of flower buds/plants (12.8), and flower diameter (6.8 cm) were found in S3. The effect of nitrogen doses and split application showed that the maximum number of flower buds/ plant (15.3) was found in N3S3, and the minimum number of flower buds/plant (8.0) was found in N1S1. The study showed that the higher nitrogen doses with split application would have the potential for Lisianthus production.
Keywords: Nitrogen management, cut flower, Floral yield, Growth and Yield
Abstract
A field experiment was conducted at the Horticulture Farm, Sher-e-Bangla Agricultural University, Dhaka, during the period from September 2025 to January 2026 to study the effect of nitrogen doses and split application schedule on growth and floral yield of Lisianthus (variety, SAU Purple Nandini). Three nitrogen doses, namely N₁ = 112 kg ha⁻¹ (BARI recommended dose for marigold), N₂ = 150 kg ha⁻¹, and N₃ = 188 kg ha⁻¹, along with three split application treatments: S₁ (full dose applied as basal), S₂ (two splits: 50% basal + 50% at 30 days after transplanting), and S₃ (three splits: 33% basal + 33% at 30 DAT + 34% at 60 DAT), were evaluated in a Randomized Complete Block Design (RCBD) with three replications. Data on different growth, flower yield, and quality attributes were recorded, and all treatments showed significant variations. Among nitrogen doses, the maximum number of flower buds/plant (14.1), flower diameter (7.6 cm), and flower stalk/ plant (4.45) were found in the N3 treatment. Among the split application maximum number of flower buds/plants (12.8), and flower diameter (6.8 cm) were found in S3. The effect of nitrogen doses and split application showed that the maximum number of flower buds/ plant (15.3) was found in N3S3, and the minimum number of flower buds/plant (8.0) was found in N1S1. The study showed that the higher nitrogen doses with split application would have the potential for Lisianthus production.
Keywords: Nitrogen management, cut flower, Floral yield, Growth and Yield
