J. Biosci. Agric. Res. | Volume 34, Issue 02, 2665-2674 | https://doi.org/10.18801/jbar.340224.329
Article type: Research article | Received: 30.05.2024; Revised: 20.03.2025; First published online: 15 May, 2025
Article type: Research article | Received: 30.05.2024; Revised: 20.03.2025; First published online: 15 May, 2025
Evaluation of rice genotypes using different concentration of NaCl at different growth stages
Sultana Razia 1, Mehede Hassan Rubel 2, Shahanaz Sultana 3, Jannatul Ferdous 3 and K. M. Nasiruddin 4
1 Biotechnology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh-2202, Bangladesh
2 Department of Agriculture, Noakhali Science and Technology University, Sonapur-3814, Bangladesh.
3 Biotechnology Division, Bangladesh Rice Research Institute, Joydebpur, Gazipur, Bangladesh.
4 Biotechnology Department, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
✉ Corresponding author: [email protected] (Sultana, S.)
1 Biotechnology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh-2202, Bangladesh
2 Department of Agriculture, Noakhali Science and Technology University, Sonapur-3814, Bangladesh.
3 Biotechnology Division, Bangladesh Rice Research Institute, Joydebpur, Gazipur, Bangladesh.
4 Biotechnology Department, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
✉ Corresponding author: [email protected] (Sultana, S.)
Abstract
Ten rice (Oryza sativa L.) genotypes viz., Pokkali, Nonabokra, Chotolona, Rajashail, FL478, FL378, BRRI dhan29, BRRI dhan40, BRRI dhan41 and BRRI dhan47 were screened against different salinity levels (50, 100, 150 and 200 mM) at germination, seedling and reproductive stages. The lab experiment was laid out in a Completely Randomized Design (CRD) with three replications and five treatments. Germination was completely inhibited and seedlings died at 200 mM. Growth inhibition to salt stress is more prominent in seedling stage than reproductive stage. Germination percentage, vigour index, shoot length and root length, fresh weight, dry weight, total tiller number per plant, spikelets number per panicle and grain weight etc., were reduced at the lowest salinity level i.e., 50 mM and significant reduction of these agronomic parameters with increasing salt concentration. Among yield attributing traits sterility showed a major cause of yield losses under saline stress. Rice genotypes Nonabokra, FL478, BRRI dhan40, BRRI dhan41 and BRRI dhan47 showed higher salt tolerance than Rajashail and Chotolona at different growth stages of rice plant. Also, tolerant genotypes showed lower Na/K, Na/Ca and Na/Mg in plant shoots than susceptible plants. Whereas, two selected SSR markers i.e., RM6317 and RM168 identified five genotypes as tolerant (FL478, FL378, BRRI dhan47, BRRI dhan41 and BRRI dhan40) compared to Pokkali at all three stages and two as susceptible (Rajashail and Chotolona) in comparison with BRRIdhan29. The result suggested that tolerant genotypes could be used for further study in screening process at different growth stage under salt stress.
Key Words: Rice, salt stress, germination, seedling stage - reproductive stage and SSR.
Ten rice (Oryza sativa L.) genotypes viz., Pokkali, Nonabokra, Chotolona, Rajashail, FL478, FL378, BRRI dhan29, BRRI dhan40, BRRI dhan41 and BRRI dhan47 were screened against different salinity levels (50, 100, 150 and 200 mM) at germination, seedling and reproductive stages. The lab experiment was laid out in a Completely Randomized Design (CRD) with three replications and five treatments. Germination was completely inhibited and seedlings died at 200 mM. Growth inhibition to salt stress is more prominent in seedling stage than reproductive stage. Germination percentage, vigour index, shoot length and root length, fresh weight, dry weight, total tiller number per plant, spikelets number per panicle and grain weight etc., were reduced at the lowest salinity level i.e., 50 mM and significant reduction of these agronomic parameters with increasing salt concentration. Among yield attributing traits sterility showed a major cause of yield losses under saline stress. Rice genotypes Nonabokra, FL478, BRRI dhan40, BRRI dhan41 and BRRI dhan47 showed higher salt tolerance than Rajashail and Chotolona at different growth stages of rice plant. Also, tolerant genotypes showed lower Na/K, Na/Ca and Na/Mg in plant shoots than susceptible plants. Whereas, two selected SSR markers i.e., RM6317 and RM168 identified five genotypes as tolerant (FL478, FL378, BRRI dhan47, BRRI dhan41 and BRRI dhan40) compared to Pokkali at all three stages and two as susceptible (Rajashail and Chotolona) in comparison with BRRIdhan29. The result suggested that tolerant genotypes could be used for further study in screening process at different growth stage under salt stress.
Key Words: Rice, salt stress, germination, seedling stage - reproductive stage and SSR.
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MLA
Razia et al. "Evaluation of rice genotypes using different concentration of NaCl at different growth stages". Journal of Bioscience and Agriculture Research, 34(02), (2025): 2746-2757.
APA
Razia. S, Rubel, M. H., Sultana, S., Ferdous, J. and Nasiruddin, K. M. (2025). Evaluation of rice genotypes using different concentration of NaCl at different growth stages. Journal of Bioscience and Agriculture Research, 34(02), 2746-2757.
Chicago
Razia. S, Rubel, M. H., Sultana, S., Ferdous, J. and Nasiruddin, K. M. ''Evaluation of rice genotypes using different concentration of NaCl at different growth stages''. Journal of Bioscience and Agriculture Research, 34(02), (2025): 2746-2757.
Harvard
Razia. S, Rubel, M. H., Sultana, S., Ferdous, J. and Nasiruddin, K. M. 2025. Evaluation of rice genotypes using different concentration of NaCl at different growth stages. Journal of Bioscience and Agriculture Research, 34(02), pp. 2746-2757.
Vancouver
Razia S, Rubel MH, Sultana S, Ferdous J and Nasiruddin KM. valuation of rice genotypes using different concentration of NaCl at different growth stages. Journal of Bioscience and Agriculture Research, 2025 May, 34(02): 2746-2757.
Razia et al. "Evaluation of rice genotypes using different concentration of NaCl at different growth stages". Journal of Bioscience and Agriculture Research, 34(02), (2025): 2746-2757.
APA
Razia. S, Rubel, M. H., Sultana, S., Ferdous, J. and Nasiruddin, K. M. (2025). Evaluation of rice genotypes using different concentration of NaCl at different growth stages. Journal of Bioscience and Agriculture Research, 34(02), 2746-2757.
Chicago
Razia. S, Rubel, M. H., Sultana, S., Ferdous, J. and Nasiruddin, K. M. ''Evaluation of rice genotypes using different concentration of NaCl at different growth stages''. Journal of Bioscience and Agriculture Research, 34(02), (2025): 2746-2757.
Harvard
Razia. S, Rubel, M. H., Sultana, S., Ferdous, J. and Nasiruddin, K. M. 2025. Evaluation of rice genotypes using different concentration of NaCl at different growth stages. Journal of Bioscience and Agriculture Research, 34(02), pp. 2746-2757.
Vancouver
Razia S, Rubel MH, Sultana S, Ferdous J and Nasiruddin KM. valuation of rice genotypes using different concentration of NaCl at different growth stages. Journal of Bioscience and Agriculture Research, 2025 May, 34(02): 2746-2757.
References:
[1]. Abou El Karam, S., Ferrand, M., Astruc, T. and Germond, A. (2023). Evaluation and prediction of salt effects on pig muscle by deep UV and machine learning. Meat Science, 199, 109136. https://doi.org/10.1016/j.meatsci.2023.109136
[2]. Ahmadi, N., Negrao, S., Courtois, B., Abreu, I., Saibo, N. and Oliveira, M. M. (2006). Recent updates on salinity stress in rice: from physiological to molecular responses. Critical Reviews in Plant Sciences, 30, 329–377. https://doi.org/10.1080/07352689.2011.587725
[3]. Aschli, F., Wopereis, and Marco, C. S. (2001). Responses of Field-grown irrigated rice cultivars to varying levels of floodwater salinity in a semi-arid environment. Field Crops Research, 70: 127-137.
[4]. Baloch, W. A., Soomro, A. M., Javed, M. A., Bughio, H. R., Alam, S. M., Bughio, M. S., Mohammed, T. and Mastoi, N. N. (2003). Introduction of salt tolerance in rice through mutation breeding. Asian Journal of Plant Science, 2, 273-276. https://doi.org/10.3923/ajps.2003.273.276
[5]. Bangladesh Bureau of Statistics (BBS). (2016). Yearbook of agricultural statistics-2015. Ministry of Planning. Government of the People’s Republic of Bangladesh, Dhaka.
[6]. Bhuiyan, M. A. R. (2005). Efficiency in evaluating salt tolerance in rice using phenotypic and marker assisted selection. M.S. Thesis, Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh, Bangladesh 96.
[7]. Cabot, C., Sibole, J. V., Barcelo, J. and Poschenrieder, C. (2009). Abscisic acid decreases leaf Na+ exclusion in salt-treated Phaseolus vulgaris L. J. Plant Growth Regulation, 28: 187–192. https://doi.org/10.1007/s00344-009-9088-5
[8]. Carpici, E. B., Celik N. and Bayram, G. (2010). Yield and quality of forage maize as influenced by plant density and nitrogen rate. Turkish Journal of Field Crops, 15, 128–132.
[9]. Falah, A. (2010). The effects of salinity at different growth stage on rice. Proceeding of 11th national congress on agronomy.
[10]. Gregorio, G. B, Senadhira, D. and Mendoza R. D. (1997). Screening rice for salinity tolerance. IRRI Discussion Paper Series no. 22. Manila (Philippines): International Rice Research Institute 1-30.
[11]. IRRI, (2013). Standardization evaluation system for rice. International Rice Research Institute, Philippines, p. 34.
[12]. Islam, M. M. (2004). Mapping salinity tolerance genes in rice (Oryza satiya L.) at reproductive stage. Ph. D. Dissertation. University of the Philippines Los Banos, College, Laguna, Philippines, pp. 1-149.
[13]. Lafitte, H. R., Price, A. H. and Courtois, B. (2004). Yield response to water deficit in an upland rice mapping population: associations among traits and genetic markers. Theoretical and Applied Genetics, 109, 1237-1246.
[14]. Lang, N. T., Yanagihara, S. and Buu, B. C. (2000). Quantitative trait loci for salt tolerance in rice via molecular markers Oman Rice 8, 37-48.
[15]. Ma, N. L., Lah, W. A. C., Kadir, N. A., Mustaqim, M., Rahmat, Z., Ahmad, A. and Ismail, M. R. (2018). Susceptibility and tolerance of rice crop to salt threat: Physiological and metabolic inspections. PLoS ONE, 13(2): 1–17. https://doi.org/10.1371/journal.pone.0192732
[16]. McNeilly and Rao, S. A. T. (1999). Genetic basis of variation for salt tolerance in maize (Zea mays L). Euphytica, 108 (3), 145–150. https://doi.org/10.1023/A:1003612411293
[17]. Nguyen, T., Yanagihara, S. and Buu, B. C. (2001). A microsatellite marker for a gene conferring salt tolerance on rice at the vegetative and reproductive stages. SABRAO J. Breed. Genet., 33, 11-20.
[18]. Niones, J. M. (2004). Fine mapping of the salinity tolerance gene on chromosome1 of rice (Oryza sativa L.) using near-isogenic lines. M.S. Dissertation. University of the Philippines, Los Baños, Philippines 78
[19]. Rao, S., Mishra, B., Gupta, S. R., and Rathore, A. (2013). Physiological response to salinity and alkalinity of rice genotypes of varying salt tolerance grown in field Lysimeters. Journal of Stress Physiology & Biochemistry, 9(1), 54-65.
[20]. Razzaque, S., Haque, T., Elias, S. M., Rahman, M. Z., Biswas, S., Schwartz, S., Ismail, A. M., Walia, H., Juenger, T. E. and Seraja, Z. I. (2017). Reproductive stage physiological and transcriptional responses to salinity stress in reciprocal populations derived from tolerant (Horkuch) and susceptible (IR29) rice. Scientific Reports, 7, 46138. https://doi.org/10.1038/srep46138
[21]. Saadat, S., Homaei, M., and Liaghat, A. (2005). Effect of salinity on growth. Journal of Soil Science and Plant Nutrition, 19(2), 243-254.
[22]. Safitri, H., Purwoko, B. S., Dewi, I. S. and Ardie, S. W. (2018). Salinity tolerance of several rice genotypes at seedling stage. International Journal of Agricultural Science, 18, 63-68. https://doi.org/10.21082/ijas.v18n2.2017.p63-68
[23]. Saqib, Z. A., Akhtar, J., Ul-Haq, M. and Ahmad, I. (2012). Salt induced changes in leaf phenology of wheat plants are regulated by accumulation and distribution pattern of Na+ ion. Pak. J. Agri. Sci, 49, 141-148.
[24]. Shereen, A., Mumtaz, S., Raza, S., Khan, M. A. and Solangi, S. (2005). Salinity effects on seedling growth and yield components of different inbred rice lines. Pakistan Journal of Botany, 37, 131-139.
[25]. Soubir, Bhowmik, S. K., Islam, M. M., Siddika, A., Sultana, S. and Haque, M. S. (2009). Phenotypic and Genotypic Screening of Rice Genotypes at Seedling Stage for Salt Tolerance. Global Journal of Biotechnology & Biochemistry, 4 (2), 126-131.
[26]. Sritharan, N. and Mallika, V. (2006). Screening rice genotypes for salt tolerance. PI. Arch., 818.
[27]. Talukder, B. and Saifuzzaman, M. (2016). Sustainability of agricultural systems in the coastal zone of Bangladesh. Renewable Agriculture and Food Systems, 31(2), 148-165. https://doi.org/10.1017/S1742170515000095
[28]. Yoshida, S., Forno, D. A., Cook, J. H. and Gomez, K. A. (1976). Laboratory manual for physiological studies of rice. Los Banos, Laguna, Philippines. International Rice Research Institute (IRRI), pp. 61-66.
[29]. Yoshida, S., Satake, T. and Mackill, D. S. (1981). High temperature stress in rice, IRRI Research Paper Series, Vol. 67
[30]. Young, C. W., Kyuscong, L., Cheo, K. J., Yeol, C. S. and Hyang, C. D. (2003). Critical saline concentration of soil and water for rice cultivation on a reclaimed saline soil. Korean journal of crop science, 48, 238-242.
[31]. Zeng, L. H. (2004). Response and correlated response of yield parameters to selection for salt tolerance in rice. Cereal Research Communications, 32, 477-484. https://doi.org/10.1007/BF03543338
[32]. Zeng, L. H. and Shannon, M. C. (2000). Salinity effects on seedling growth and yield components of rice. Crop Science, 40(4), 996–1003. https://doi.org/10.2135/cropsci2000.404996x
[33]. Zhao, B. T., Ge, L. F., Liang, R. Q., Li, W., Ruan, K. C., Lin, H. X. and Jin, Y. X. (2009). Members of miR-169 family are induced by high salinity and transiently inhibit the NFYA transcription factor. BMC Molecular Biology, 10, 29p. https://doi.org/10.1186/1471-2199-10-29
[2]. Ahmadi, N., Negrao, S., Courtois, B., Abreu, I., Saibo, N. and Oliveira, M. M. (2006). Recent updates on salinity stress in rice: from physiological to molecular responses. Critical Reviews in Plant Sciences, 30, 329–377. https://doi.org/10.1080/07352689.2011.587725
[3]. Aschli, F., Wopereis, and Marco, C. S. (2001). Responses of Field-grown irrigated rice cultivars to varying levels of floodwater salinity in a semi-arid environment. Field Crops Research, 70: 127-137.
[4]. Baloch, W. A., Soomro, A. M., Javed, M. A., Bughio, H. R., Alam, S. M., Bughio, M. S., Mohammed, T. and Mastoi, N. N. (2003). Introduction of salt tolerance in rice through mutation breeding. Asian Journal of Plant Science, 2, 273-276. https://doi.org/10.3923/ajps.2003.273.276
[5]. Bangladesh Bureau of Statistics (BBS). (2016). Yearbook of agricultural statistics-2015. Ministry of Planning. Government of the People’s Republic of Bangladesh, Dhaka.
[6]. Bhuiyan, M. A. R. (2005). Efficiency in evaluating salt tolerance in rice using phenotypic and marker assisted selection. M.S. Thesis, Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh, Bangladesh 96.
[7]. Cabot, C., Sibole, J. V., Barcelo, J. and Poschenrieder, C. (2009). Abscisic acid decreases leaf Na+ exclusion in salt-treated Phaseolus vulgaris L. J. Plant Growth Regulation, 28: 187–192. https://doi.org/10.1007/s00344-009-9088-5
[8]. Carpici, E. B., Celik N. and Bayram, G. (2010). Yield and quality of forage maize as influenced by plant density and nitrogen rate. Turkish Journal of Field Crops, 15, 128–132.
[9]. Falah, A. (2010). The effects of salinity at different growth stage on rice. Proceeding of 11th national congress on agronomy.
[10]. Gregorio, G. B, Senadhira, D. and Mendoza R. D. (1997). Screening rice for salinity tolerance. IRRI Discussion Paper Series no. 22. Manila (Philippines): International Rice Research Institute 1-30.
[11]. IRRI, (2013). Standardization evaluation system for rice. International Rice Research Institute, Philippines, p. 34.
[12]. Islam, M. M. (2004). Mapping salinity tolerance genes in rice (Oryza satiya L.) at reproductive stage. Ph. D. Dissertation. University of the Philippines Los Banos, College, Laguna, Philippines, pp. 1-149.
[13]. Lafitte, H. R., Price, A. H. and Courtois, B. (2004). Yield response to water deficit in an upland rice mapping population: associations among traits and genetic markers. Theoretical and Applied Genetics, 109, 1237-1246.
[14]. Lang, N. T., Yanagihara, S. and Buu, B. C. (2000). Quantitative trait loci for salt tolerance in rice via molecular markers Oman Rice 8, 37-48.
[15]. Ma, N. L., Lah, W. A. C., Kadir, N. A., Mustaqim, M., Rahmat, Z., Ahmad, A. and Ismail, M. R. (2018). Susceptibility and tolerance of rice crop to salt threat: Physiological and metabolic inspections. PLoS ONE, 13(2): 1–17. https://doi.org/10.1371/journal.pone.0192732
[16]. McNeilly and Rao, S. A. T. (1999). Genetic basis of variation for salt tolerance in maize (Zea mays L). Euphytica, 108 (3), 145–150. https://doi.org/10.1023/A:1003612411293
[17]. Nguyen, T., Yanagihara, S. and Buu, B. C. (2001). A microsatellite marker for a gene conferring salt tolerance on rice at the vegetative and reproductive stages. SABRAO J. Breed. Genet., 33, 11-20.
[18]. Niones, J. M. (2004). Fine mapping of the salinity tolerance gene on chromosome1 of rice (Oryza sativa L.) using near-isogenic lines. M.S. Dissertation. University of the Philippines, Los Baños, Philippines 78
[19]. Rao, S., Mishra, B., Gupta, S. R., and Rathore, A. (2013). Physiological response to salinity and alkalinity of rice genotypes of varying salt tolerance grown in field Lysimeters. Journal of Stress Physiology & Biochemistry, 9(1), 54-65.
[20]. Razzaque, S., Haque, T., Elias, S. M., Rahman, M. Z., Biswas, S., Schwartz, S., Ismail, A. M., Walia, H., Juenger, T. E. and Seraja, Z. I. (2017). Reproductive stage physiological and transcriptional responses to salinity stress in reciprocal populations derived from tolerant (Horkuch) and susceptible (IR29) rice. Scientific Reports, 7, 46138. https://doi.org/10.1038/srep46138
[21]. Saadat, S., Homaei, M., and Liaghat, A. (2005). Effect of salinity on growth. Journal of Soil Science and Plant Nutrition, 19(2), 243-254.
[22]. Safitri, H., Purwoko, B. S., Dewi, I. S. and Ardie, S. W. (2018). Salinity tolerance of several rice genotypes at seedling stage. International Journal of Agricultural Science, 18, 63-68. https://doi.org/10.21082/ijas.v18n2.2017.p63-68
[23]. Saqib, Z. A., Akhtar, J., Ul-Haq, M. and Ahmad, I. (2012). Salt induced changes in leaf phenology of wheat plants are regulated by accumulation and distribution pattern of Na+ ion. Pak. J. Agri. Sci, 49, 141-148.
[24]. Shereen, A., Mumtaz, S., Raza, S., Khan, M. A. and Solangi, S. (2005). Salinity effects on seedling growth and yield components of different inbred rice lines. Pakistan Journal of Botany, 37, 131-139.
[25]. Soubir, Bhowmik, S. K., Islam, M. M., Siddika, A., Sultana, S. and Haque, M. S. (2009). Phenotypic and Genotypic Screening of Rice Genotypes at Seedling Stage for Salt Tolerance. Global Journal of Biotechnology & Biochemistry, 4 (2), 126-131.
[26]. Sritharan, N. and Mallika, V. (2006). Screening rice genotypes for salt tolerance. PI. Arch., 818.
[27]. Talukder, B. and Saifuzzaman, M. (2016). Sustainability of agricultural systems in the coastal zone of Bangladesh. Renewable Agriculture and Food Systems, 31(2), 148-165. https://doi.org/10.1017/S1742170515000095
[28]. Yoshida, S., Forno, D. A., Cook, J. H. and Gomez, K. A. (1976). Laboratory manual for physiological studies of rice. Los Banos, Laguna, Philippines. International Rice Research Institute (IRRI), pp. 61-66.
[29]. Yoshida, S., Satake, T. and Mackill, D. S. (1981). High temperature stress in rice, IRRI Research Paper Series, Vol. 67
[30]. Young, C. W., Kyuscong, L., Cheo, K. J., Yeol, C. S. and Hyang, C. D. (2003). Critical saline concentration of soil and water for rice cultivation on a reclaimed saline soil. Korean journal of crop science, 48, 238-242.
[31]. Zeng, L. H. (2004). Response and correlated response of yield parameters to selection for salt tolerance in rice. Cereal Research Communications, 32, 477-484. https://doi.org/10.1007/BF03543338
[32]. Zeng, L. H. and Shannon, M. C. (2000). Salinity effects on seedling growth and yield components of rice. Crop Science, 40(4), 996–1003. https://doi.org/10.2135/cropsci2000.404996x
[33]. Zhao, B. T., Ge, L. F., Liang, R. Q., Li, W., Ruan, K. C., Lin, H. X. and Jin, Y. X. (2009). Members of miR-169 family are induced by high salinity and transiently inhibit the NFYA transcription factor. BMC Molecular Biology, 10, 29p. https://doi.org/10.1186/1471-2199-10-29
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