Journal of Bioscience and Agriculture Research
An Investigation to Virus Like Diseases of Marigold
R. Sultana 1, A. M. Akanda 2, M. A. S. Haque 3, A. Majumdar 4 and M. A. Z. Al
Munsur 4
1 Dept. of Agriculture Extension, Ministry of Agriculture (MOA), Khamarbari, Dhaka
2 Dept. of Plant Pathology, BSMRAU, Gazipur
3 International Development Enterprises, Bangladesh
4 Dept. of Agriculture Extension, MOA, Khamarbari, Dhaka, Bangladesh.
DOI: https://doi.org/10.18801/jbar.020114.16
Munsur 4
1 Dept. of Agriculture Extension, Ministry of Agriculture (MOA), Khamarbari, Dhaka
2 Dept. of Plant Pathology, BSMRAU, Gazipur
3 International Development Enterprises, Bangladesh
4 Dept. of Agriculture Extension, MOA, Khamarbari, Dhaka, Bangladesh.
DOI: https://doi.org/10.18801/jbar.020114.16
Abstract
A study was conducted to identify the cause of virus disease-like symptoms developed naturally in marigold (Tagetes erecta L.) plants in Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur.The natural symptoms in marigold consisted of yellowing, mosaic, shoestring, mottling and curling of leaves resulting sever stunting of the infected plants. The ailments were found to be sap transmissible to marigold inducing identical symptoms as observed in naturally infected plants. Double Antibody Sandwich Enzyme-Linked Immuno- Sorbent Assay (DAS-ELISA) and Indirect Enzyme-Linked Immuno-Sorbent Assay (I-ELISA) detected the mixed infections of Cucumber mosaic virus (CMV) and Papaya ringspot virus (PRSV) from the naturally infected marigold plants. Chenopodium amaranticolor and Gomphrena globosa were found to be good local lesion host producing chlorotic and necrotic local lesions in the inoculated leaves respectively. The pure isolates obtained from the local lesion by using Chenopodium amaranticolor when used for host range test. It was observed that the virus had wide host range which included the plants of Amaranthaceae, Chenopodiaceae, Compositae, Cucurbitaceae, Leguminosae, Malvaceae and Solanaceae. The dilution end point, thermal inactivation point and longevity in vitro were determined as 10-6, 65C and 9 days, respectively. Moreover, the pure isolate obtained from the local lesion did not produce some symptoms like shoestring in marigold which observed in naturally infected marigold plants. The host range test, dilution end point, thermal inactivation point and longevity in vitro revealed that the purified isolate was the CMV. The results suggested that the virus was CMV, but not PRSV from the naturally infected marigold samples. The results of the study revealed that the virus disease-like symptoms manifested in marigold plants had mixed infection of CMV and PRSV.
Key Words: Investigation, Virus, Disease, Marigold and Tagetes erecta L.
A study was conducted to identify the cause of virus disease-like symptoms developed naturally in marigold (Tagetes erecta L.) plants in Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur.The natural symptoms in marigold consisted of yellowing, mosaic, shoestring, mottling and curling of leaves resulting sever stunting of the infected plants. The ailments were found to be sap transmissible to marigold inducing identical symptoms as observed in naturally infected plants. Double Antibody Sandwich Enzyme-Linked Immuno- Sorbent Assay (DAS-ELISA) and Indirect Enzyme-Linked Immuno-Sorbent Assay (I-ELISA) detected the mixed infections of Cucumber mosaic virus (CMV) and Papaya ringspot virus (PRSV) from the naturally infected marigold plants. Chenopodium amaranticolor and Gomphrena globosa were found to be good local lesion host producing chlorotic and necrotic local lesions in the inoculated leaves respectively. The pure isolates obtained from the local lesion by using Chenopodium amaranticolor when used for host range test. It was observed that the virus had wide host range which included the plants of Amaranthaceae, Chenopodiaceae, Compositae, Cucurbitaceae, Leguminosae, Malvaceae and Solanaceae. The dilution end point, thermal inactivation point and longevity in vitro were determined as 10-6, 65C and 9 days, respectively. Moreover, the pure isolate obtained from the local lesion did not produce some symptoms like shoestring in marigold which observed in naturally infected marigold plants. The host range test, dilution end point, thermal inactivation point and longevity in vitro revealed that the purified isolate was the CMV. The results suggested that the virus was CMV, but not PRSV from the naturally infected marigold samples. The results of the study revealed that the virus disease-like symptoms manifested in marigold plants had mixed infection of CMV and PRSV.
Key Words: Investigation, Virus, Disease, Marigold and Tagetes erecta L.
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Citation for this article (APA Style)
Sultana, R., Akanda, A. M., Haque, M. A. S., Majumdar, A., Munsur, M. A. Z. Al., 2014. An Investigation to Virus Like Diseases of Marigold. Journal of Bioscience and Agriculture Research, 02(01), 16-23.
Sultana, R., Akanda, A. M., Haque, M. A. S., Majumdar, A., Munsur, M. A. Z. Al., 2014. An Investigation to Virus Like Diseases of Marigold. Journal of Bioscience and Agriculture Research, 02(01), 16-23.
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