Journal of Bioscience and Agriculture Research |
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In Vitro Microtuberization of Potato (Solanum Tuberosum L.) Cultivar through Sucrose and Growth Regulator
K. Momena (1), R. Adeeba (1), H. Mehraj (2), A.F.M. Jamal Uddin (2), Saiful Islam (3) and L. Rahman (1)
Advanced Seed Research and Biotech Center, ACI Limited, Dhaka (1)
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka (2)
University of Development Alternative, Dhaka, Bangladesh (3)
DOI: https://doi.org/10.18801/jbar.020214.22
K. Momena (1), R. Adeeba (1), H. Mehraj (2), A.F.M. Jamal Uddin (2), Saiful Islam (3) and L. Rahman (1)
Advanced Seed Research and Biotech Center, ACI Limited, Dhaka (1)
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka (2)
University of Development Alternative, Dhaka, Bangladesh (3)
DOI: https://doi.org/10.18801/jbar.020214.22
Abstract
Microtubers which are produced in tissue culture have benefits for disease free potato production. As growth regulators and sucrose concentration affect the initiation and growth of microtuber, therefore, an experiment was conducted to discover the suitable growth supplement to produce disease free potato microtuber. Four potato cultivars viz. Granula, Daimond, Sarpoaxona and Lal Pakri1 with three different treatments viz. T1 = 8% Sucrose, T2 = 6% Sucrose + 4 mg/L Kinetin, T3 = 8% Sucrose + 4 mg/L Kinetin + 1 mg/L BAP were used for micro tuberization as supplement of Murashige and Skoog (MS) medium. Among tested cultivars, Diamond performed better compare to other three potato cultivars with treatment combination T3. Maximum number of culture showing microtuber (56.0), explant formed microtuber (93.3%), number of potato microtuber (2.7), average diameter (4.42 mm) and average weight (97.61 mg) were found from Diamond cultivar in 8% Sucrose + 4 mg/L Kinetin + 1 mg/L BAP.
Key words: Potato cultivars, Microtuberization, Sucrose, Kinetin and BAP
Microtubers which are produced in tissue culture have benefits for disease free potato production. As growth regulators and sucrose concentration affect the initiation and growth of microtuber, therefore, an experiment was conducted to discover the suitable growth supplement to produce disease free potato microtuber. Four potato cultivars viz. Granula, Daimond, Sarpoaxona and Lal Pakri1 with three different treatments viz. T1 = 8% Sucrose, T2 = 6% Sucrose + 4 mg/L Kinetin, T3 = 8% Sucrose + 4 mg/L Kinetin + 1 mg/L BAP were used for micro tuberization as supplement of Murashige and Skoog (MS) medium. Among tested cultivars, Diamond performed better compare to other three potato cultivars with treatment combination T3. Maximum number of culture showing microtuber (56.0), explant formed microtuber (93.3%), number of potato microtuber (2.7), average diameter (4.42 mm) and average weight (97.61 mg) were found from Diamond cultivar in 8% Sucrose + 4 mg/L Kinetin + 1 mg/L BAP.
Key words: Potato cultivars, Microtuberization, Sucrose, Kinetin and BAP
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Citation:
Momena, K., Adeeba, R., Mehraj H., Jamal Uddin, A. F. M., Islam, S. & Rahman L. (2014). In Vitro Microtuberization of Potato (Solanum Tuberosum L.) Cultivar through Sucrose and Growth Regulator. Journal of Bioscience and Agriculture Research 02(02), 76-82.
Momena, K., Adeeba, R., Mehraj H., Jamal Uddin, A. F. M., Islam, S. & Rahman L. (2014). In Vitro Microtuberization of Potato (Solanum Tuberosum L.) Cultivar through Sucrose and Growth Regulator. Journal of Bioscience and Agriculture Research 02(02), 76-82.