Asian J. Crop. Soil Plan. Nutri. | Volume 02, Issue 02, 62-62 | https://doi.org/10.18801/ajcsp.020220.09
Article type: Research article | Received: 27.11.19; Revised: 19.03.2020; First published online: 05 April 2020.
Article type: Research article | Received: 27.11.19; Revised: 19.03.2020; First published online: 05 April 2020.
Effect of alginate on protocorm like bodies (PLBs) formation of Dendrobium kingianum cultivar
Md Manirul Alam 1, Kazuhiko Shimasaki 2, Sultana Umma Habiba 1 and Nusrat Jahan 3
1 The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarami, Matsumaya, Ehime 790-8556, Japan.
2 Faculty of Agriculture and Marine Science, Kochi University, B200Monobe, Nankoku, Kochi 783-8502, Japan.
3 Business of Commerce, Cumilla Victoria University, Cumilla-3500, Bangladesh.
✉ Article correspondence: [email protected] (Alam, M. M.)
1 The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarami, Matsumaya, Ehime 790-8556, Japan.
2 Faculty of Agriculture and Marine Science, Kochi University, B200Monobe, Nankoku, Kochi 783-8502, Japan.
3 Business of Commerce, Cumilla Victoria University, Cumilla-3500, Bangladesh.
✉ Article correspondence: [email protected] (Alam, M. M.)
Abstract
Mass propagation provides an alternative means of satisfying the demand. Unfortunately, conventional propagation is slow and difficult, suggesting in vitro methods for mass multiplication may be more appropriate. Supplement of plant growth regulators and biopolysaccaridesom modified MS medium was effective for the growth and development of protocorm like bodies (PLBs) of Dendrobium kingianum ‘Hallelujah’. The results of this experiment revealed that the highest number of PLBs were found in the media containing 0.01mg/L of alginate and that was 17.2/explant whereas control (6.1/explant). The second maximum number of PLBs (16.9) was at concentrations of 0.01mg/L HA9. On the other hand, the high concentration of NAA; HA9 and Alginate showed negative results of PLBs formation in Dendrobium kingianum ‘Hallelujah’ except BAP. In the case of PLBs formation, 100% PLBs were formed in every low concentration of BAP, NAA and HA9 except alginate.
Key Words: Dendrobium, protocorm-like body (PLBs), 6-Benzylaminopurine (BAP), Naphthalene Acetic Acid (NAA), Hyaluronic Acid (HA9) and Sodium alginate
Mass propagation provides an alternative means of satisfying the demand. Unfortunately, conventional propagation is slow and difficult, suggesting in vitro methods for mass multiplication may be more appropriate. Supplement of plant growth regulators and biopolysaccaridesom modified MS medium was effective for the growth and development of protocorm like bodies (PLBs) of Dendrobium kingianum ‘Hallelujah’. The results of this experiment revealed that the highest number of PLBs were found in the media containing 0.01mg/L of alginate and that was 17.2/explant whereas control (6.1/explant). The second maximum number of PLBs (16.9) was at concentrations of 0.01mg/L HA9. On the other hand, the high concentration of NAA; HA9 and Alginate showed negative results of PLBs formation in Dendrobium kingianum ‘Hallelujah’ except BAP. In the case of PLBs formation, 100% PLBs were formed in every low concentration of BAP, NAA and HA9 except alginate.
Key Words: Dendrobium, protocorm-like body (PLBs), 6-Benzylaminopurine (BAP), Naphthalene Acetic Acid (NAA), Hyaluronic Acid (HA9) and Sodium alginate
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MLA
Alam, M. M. et al. “Effect of alginate on protocorm like bodies (PLBs) formation of Dendrobium kingianum cultivar”. Asian Journal of Crop, Soil Science and Plant Nutrition 02(02), (2020): 62-67.
APA
Alam, M. M., Shimasaki, K., Habiba, S. U. and Jahan N. (2020). Effect of alginate on protocorm like bodies (PLBs) formation of Dendrobium kingianum cultivar. Asian Journal of Crop, Soil Science and Plant Nutrition, 02(02), 62-67.
Chicago
Alam, M. M., Shimasaki, K., Habiba, S. U. and Jahan N. “Effect of alginate on protocorm like bodies (PLBs) formation of Dendrobium kingianum cultivar”. Asian Journal of Crop, Soil Science and Plant Nutrition 02(02), (2020): 62-67.
Harvard
Alam, M. M., Shimasaki, K., Habiba, S. U. and Jahan N. 2020. Effect of alginate on protocorm like bodies (PLBs) formation of Dendrobium kingianum cultivar. Asian Journal of Crop, Soil Science and Plant Nutrition, 02(02), pp. 62-67.
Vancouver
Alam, MM, Shimasaki, K, Habiba, SU and Jahan N. Effect of alginate on protocorm like bodies (PLBs) formation of Dendrobium kingianum cultivar. Asian Journal of Crop, Soil Science and Plant Nutrition. 2020 April 02(02): 62-67.
Alam, M. M. et al. “Effect of alginate on protocorm like bodies (PLBs) formation of Dendrobium kingianum cultivar”. Asian Journal of Crop, Soil Science and Plant Nutrition 02(02), (2020): 62-67.
APA
Alam, M. M., Shimasaki, K., Habiba, S. U. and Jahan N. (2020). Effect of alginate on protocorm like bodies (PLBs) formation of Dendrobium kingianum cultivar. Asian Journal of Crop, Soil Science and Plant Nutrition, 02(02), 62-67.
Chicago
Alam, M. M., Shimasaki, K., Habiba, S. U. and Jahan N. “Effect of alginate on protocorm like bodies (PLBs) formation of Dendrobium kingianum cultivar”. Asian Journal of Crop, Soil Science and Plant Nutrition 02(02), (2020): 62-67.
Harvard
Alam, M. M., Shimasaki, K., Habiba, S. U. and Jahan N. 2020. Effect of alginate on protocorm like bodies (PLBs) formation of Dendrobium kingianum cultivar. Asian Journal of Crop, Soil Science and Plant Nutrition, 02(02), pp. 62-67.
Vancouver
Alam, MM, Shimasaki, K, Habiba, SU and Jahan N. Effect of alginate on protocorm like bodies (PLBs) formation of Dendrobium kingianum cultivar. Asian Journal of Crop, Soil Science and Plant Nutrition. 2020 April 02(02): 62-67.
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