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JBAR | Journal of Bioscience and Agriculture Research 
Research article: 
Calcium carbonate supplementation causes preventive approach to toxic effects of mercuric chloride on metabolic regulation in liver of C. punctata
 
Md. Shahidul Haque (a), Md. Mahmudul Hasan (a) and Md. Mosharrof Hossain (b)
 
(a) Dept. of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh
(b) Dept. of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh


J. bios. agric. res., volume 06, issue 01, pp. 489-500 | Available online: 22 October 2015
DOI: http://dx.doi.org/10.18801/jbar.060115.59
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Abstract: Mercury (Hg) shows the toxic effects in the environment although the etiology is not well characterized and the prevention of toxic effects induced by Hg is an important aspect of metabolic regulation in organisms.  Channa punctata, a variety of species of fish was used in this study and the role of calcium carbonate on cholesterol, triglyceride and protein level in liver induced by HgCl2 was adopted.  Fish were exposed to 1 and 10 µM of HgCl2 for 1h and cholesterol and triglyceride levels in excised liver were enhanced in response to HgCl2 when compared to respective controls however the effects were more pronounced for 1 µM concentration.  Similar stimulatory effects on protein contents were demonstrated whenever they were exposed to HgCl2 (1 and 10 µM) and higher proteins were recorded for 10 µM concentration.  The results indicate that HgCl2 causes severe toxic effects enhancing the above parameters.  To clarify the role of CaCO3 on prevention of these effects, fish were treated with different concentrations (100 µM and 1 mM) of CaCO3 and CaCO3 + HgCl2.  Cholesterol and triglyceride in liver were effectively reduced with CaCO3 (1 mM) + HgCl2 (10 µM) and CaCO3 (100 µM) + HgCl2 (1 µM) while 100 µM of CaCO3 potentially reduced the effects of HgCl2. Although CaCO3 was shown to reduce protein content effectively, however 100 µM concentrations have been found to inhibit the effects of HgCl2 preferentially.  Our findings suggest that calcium carbonate might be involved in prevention of the toxic effects of Hg and may contribute to the survival process of this species.
Key words: Channa punctate, toxic effects, liver, metabolic regulation and mercuric chloride
APA (American Psychological Association)
Haque, M. S., Hasan, M. M. & Hossain, M. M. (2015). Calcium carbonate supplementation causes preventive approach to toxic effects of mercuric chloride on metabolic regulation in liver of C. punctata. Journal of Bioscience and Agriculture Research, 06 (01), 489-500.
 
MLA (Modern Language Association) 
Haque, M. S., Hasan, M. M. & Hossain, M. M. “Calcium carbonate supplementation causes preventive approach to toxic effects of mercuric chloride on metabolic regulation in liver of C. punctata.” Journal of Bioscience and Agriculture Research, 06.01 (2015): 489-500.
 
Chicago/Turabian
Haque, M. S., Hasan, M. M. & Hossain, M. M.  “Calcium carbonate supplementation causes preventive approach to toxic effects of mercuric chloride on metabolic regulation in liver of C. punctata.” Journal of Bioscience and Agriculture Research, 06, no. 01 (2015): 489-500.
  1. ​Al-Whaibi, M. H. (2011). Plant heat-shock proteins: A mini review. Journal of King Saud University - Science, 23(2), 139-150.
  2. Ahmad, I., Maria, V. L., Oliveira, M., Pacheco, M. & Santos, M. A. (2006).. Oxidative stress and genotoxic effects in gill and kidney of Anguilla anguilla L. exposed to chromium with or without preexposure to b-naphthoflavone. Mutation Research, 608, 16-28.​ http://dx.doi.org/10.1016/j.mrgentox.2006.04.020
  3. Alvarez, M. D., Murphy, C. A., Rose, K. A., McCarthy, I. D. & Fuiman, L. A. (2006). Maternal body burdens of methylmercury impair survival skills of offspring in Atlantic croaker (Micropogonias undulatus). Aquatic Toxicology, 80, 329-337.​ http://dx.doi.org/10.1016/j.aquatox.2006.09.010
  4. Bainy, A. C. D. (1996). Oxidative stress as biomarker of polluted aquatic sites. In: Val AL, Almeida-Val VMF, Randall DJ (eds) Physiology and Biochemistry of the fishes of the Amazon. INPA, Manaus, pp. 101-110.
  5. Black, F. J., Bruland, K. W., Flegal, A. R. (2007). Competing ligand exchangesolid phase extraction method for the determination of the complexation of dissolved inorganic mercury (II) in natural waters. Analytica Chimica Acta, 598, 318-333.​ http://dx.doi.org/10.1016/j.aca.2007.07.043
  6. Banerjee, B. D., Seth, V., Bhattacharya, A., Pasha, S. T. & Chakraborty, A. K. (1999). Biochemical effects of some pesticides on lipid peroxidation and free-radical scavengers. Toxicology Letters, 107, 33-47.
  7. Chen, C. Y., Dionne, M., Mayes, B. M., Ward, D. M., Sturup, S. & Jackson, B. P. (2009). Mercury bioavailability and bioaccumulation in estuarine food webs in the Gulf of Maine. Environmental Science & Technology, 43, 1804-1810. http://dx.doi.org/10.1021/es8017122
  8. Carta, P., Flore, C., Alinovi, R., Ibba, A., Tocco, M. G., Aru, G., Carta, R., Girei, E., Mutti, A., Lucchini, R. & Randaccio, F. S. (2003). Sub-clinical neurobehavioral abnormalities associated with low level of mercury exposure through fish consumption. Neurotoxicology, 24, 617-623.​ http://dx.doi.org/10.1016/S0161-813X(03)00080-9
  9. Das, S. C. S. & Datta, S. (2008). Effect of contact time on the acute toxicity of mercury to scale carp. Journal of Indian Fisheries Association, 35, 113-120.
  10. Datta, S., Singh, A. & Das, R. C. (2003).  Influence of soil sediment factors on the acute toxicity of inorganic mercury to Catla catla. Environment and Ecology, 21(3), 542-551.
  11. Elia, A. C., Galarini, R., Taticchi, M. I., Do¨rra, A. J. M. & Mantilacci, L. (2003). Antioxidant responses and bioaccumulation in Ictalurus melas under mercury exposure. Ecotoxicology and Environmental Safety, 55, 162-167. http://dx.doi.org/10.1016/S0147-6513(02)00123-9
  12. Giudetti, A. M., Damiano, F., Gnoni, G. V. & Siculella, L. (2013).  Low level of hydrogen peroxide induces lipid synthesis in BRL-3A cells through a CAP-independent SREBP-1a activation. International Journal of Biochemistry and Cell Biology, 45, 1419-1426.​ http://dx.doi.org/10.1016/j.biocel.2013.04.004
  13. Jackman, A. P., Kennedy, V. C. & Bhatia, N. (2001). Interparticle migration of metal cations in stream sediments as a factor in toxics transport. Journal of Hazardous Materials, 82(1): 27-41.​ http://dx.doi.org/10.1016/S0304-3894(00)00349-6
  14. Kenny, A. P. (1952). The determination of cholesterol by the Liebermann Burchard reaction. Biochemical Journal, 52(4), 611-619. http://dx.doi.org/10.1042/bj0520611
  15. Lowry, O. H., Rosenbrough, N. J. & Randall, R. J. (1951). Protein measurement with the Folin-phenol reagent. Journal of Biological Chemistry, 183, 265-275.
  16. Lopez, P. A., Pinheiro, T., Santos, M. C., Mathias, M. L., Collares-Pereira, M. J. & Viesgas-Crespo, A. M. (2001). Response of antioxidant enzymes in freshwater fish populations (Leucicus alburnoides complex) to inorganic pollutants exposure. Sci. Total Environ., 280, 153-163.
  17. Li, Z. H., Chen, L., Wu, Y. H., Li, P., Li, Y. F. & Ni, Z. H. (2014). Effects of mercury on oxidative stress and gene expression of potential biomarkers in larvae of the Chinese rare minnow Gobiocypris rarus. Arch Environ Contam Toxicology, 67(2), 245-51.​ http://dx.doi.org/10.1007/s00244-014-0034-6
  18. Oliveira Ribeiro, C. A., Gumara˜es, J. R. D. & Pfeiffer, C. (1996). Accumulation and distribution of inorganic mercury in a tropical fish (Trichomycterus zonatus). Ecotoxicology and Environmental Safety, 34, 190-195. http://dx.doi.org/10.1006/eesa.1996.0063
  19. Padmini, E., Hepshibha, B. T. & Shellomith, A. S. S. (2004). Aquaculture, 5(1): 115-118.
  20. Roy, S. K. & Haque, M. S. (2009). Interaction of arsenic on cold-induced adaptive response involving the change of liver weight of fish Channa punctatus. Bangladesh Journal of Medical Science, 15(2), 115-119.
  21. Rothschild, R. F. & Duffy, L. K. (2005). Mercury concentrations in muscle, brain and bone of Western Alaskan waterfowl. Science of the Total Environment, 349: 277-283.​ http://dx.doi.org/10.1016/j.scitotenv.2005.05.021
  22. Rangwala, S. M. & Lazar, M. A. (2000). Transcriptional control of adipogenesis. Annual Review of Nutrition, 20, 535-559. http://dx.doi.org/10.1146/annurev.nutr.20.1.535
  23. Rosen, E. D., Walkey, C. J., Puigserver, P. & Spiegelman, B. M. (2000). Transcriptional regulation of adipogenesis. Genes & Development, 14, 1293-1307.
  24. Risher, J. F. & Amler, S. N. (2005). Mercury exposure: evaluation and intervention the inappropriate use of chelating agents in the diagnosis and treatment of putative mercury poisoning. Neurotoxicology, 26, 691-699. http://dx.doi.org/10.1016/j.neuro.2005.05.004
  25. Shastri, Y. & Diwekar, U. (2008). Optimal control of lake pH for mercury bioaccumulation control. Ecological Modelling, 216, 1-17. http://dx.doi.org/10.1016/j.ecolmodel.2008.03.019
  26. Ung, C. Y., Lam, S. H., Hlaing, M. M., Winata, C. L., Korzh, S., Mathavan, S. & Gong, Z. (2010). Mercury-induced hepatotoxicity in zebrafish: in vivo mechanistic insights from transcriptome analysis, phenotype anchoring and targeted gene expression validation.  BMC Genomics, 11, 212. http://dx.doi.org/10.1186/1471-2164-11-212
  27. Verlecar, X. N., Jena, K. B. & Chainy, G. B. N. (2007). Biochemical markers of oxidative stress in Perna viridis exposed to mercury and temperature. Chemico-Biological Interactions, 167, 219-226. http://dx.doi.org/10.1016/j.cbi.2007.01.018
  28. Verlecar, X. N., Jena, K. B. & Chainy, G. B. N. (2008). Modulation of antioxidant defences in digestive gland of Perna viridis (L.), on mercury exposures. Chemosphere, 71, 1977-1985.​ http://dx.doi.org/10.1016/j.chemosphere.2007.12.014
  29. Zhang, L. & Wong, M. H. (2007). Environmental mercury contamination in China: sources and impacts. Environment International, 33, 108-121.​ http://dx.doi.org/10.1016/j.envint.2006.06.022
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