Journal of Bioscience and Agriculture Research |
|
Research article:
RAPD analysis of phomopsis fruit rot resistance in eggplant advanced lines
Marzia Bilkiss (a), Rahat Akhter (a), Mirza Mofazzal Islam (b), M. Ibrahim Khalil (c) and M. Bahadur Meah (a)
aDept. of Plant Pathology, Bangladesh Agricultural University, Mymensingh
bPlant Breeding Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh
cPlant Pathology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh
J. bios. agric. res., volume 06, issue 01, pp. 530-540 | Available online: 14 January 2016
DOI: http://dx.doi.org/10.18801/jbar.060116.63
RAPD analysis of phomopsis fruit rot resistance in eggplant advanced lines
Marzia Bilkiss (a), Rahat Akhter (a), Mirza Mofazzal Islam (b), M. Ibrahim Khalil (c) and M. Bahadur Meah (a)
aDept. of Plant Pathology, Bangladesh Agricultural University, Mymensingh
bPlant Breeding Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh
cPlant Pathology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh
J. bios. agric. res., volume 06, issue 01, pp. 530-540 | Available online: 14 January 2016
DOI: http://dx.doi.org/10.18801/jbar.060116.63
rapd_analysis_of_phomopsis_fruit_rot_resistance_in_eggplant_advanced_lines_version__1.1_.pdf |
Abstract: Ten lines of eggplant (F3 to F7) derived from the crossing BAU Begun-1(resistant variety) with Dohazari G and Laffa S (susceptible cultivars) were evaluated for disease resistance and genotypic performances. Two lines of different characters from each generations were selected and inoculated with Phomopsis vexans under confined field condition at fruiting stage. The inoculated genotypes exhibited differential disease reaction. Per-cent fruit infection ranged from 2.77% to 4.1%. All the lines were found to be resistant to phomopsis fruit rot indicating the inheritance of resistance trait of donor parent BAU begun-1 to the offspring. Random Amplified Polymorphic DNA (RAPD) technique was used as a tool for assessing genetic variation and relationships among the genotypes. Amplification with three decamer primers generated 13 bands of which 11 (84.62%) were polymorphic. The highest intra-variety similarity indices (Si) value was found for Laffa purple globose of F7 plants and Dohazari green long of F4. The coefficient of gene differentiation (Gst) was 1.00 reflecting the existence of high level of genetic variations among the genotypes. Comparatively higher genetic distance (0.7466) was observed between Dohazari green long of F6 vs Laffa green globose of F6 and the higher genetic identity (0.9870) was found in Dohazari green long of F6 vs Laffa green globose of F5. The dendrogram (UPGMA) constructed from Nei’s genetic distance produced two main clusters of the ten genotypes. The advanced lines can be released as variety after yield trial and also the genetic variation and relatedness to disease reaction as assessed using RAPD marker could be potential sources for further development of P. vexans resistant variety in terms of fruit color and shape.
Key words: Eggplant, Fruit rot resistance, Phomopsis vexans and RAPD
Key words: Eggplant, Fruit rot resistance, Phomopsis vexans and RAPD
APA (American Psychological Association)
Bilkiss, M., Akhter, R., Islam, M. M., Khalil, M. I. & Meah, M. B. (2016). RAPD analysis of phomopsis fruit rot resistance in eggplant advanced lines. Journal of Bioscience and Agriculture Research, 06(01), 530-540.
MLA (Modern Language Association)
Bilkiss, M., Akhter, R., Islam, M. M., Khalil, M. I. & Meah, M. B. “RAPD analysis of phomopsis fruit rot resistance in eggplant advanced lines.” Journal of Bioscience and Agriculture Research, 06.01 (2016): 530-540.
Chicago/Turabian
Bilkiss, M., Akhter, R., Islam, M. M., Khalil, M. I. & Meah, M. B. “RAPD analysis of phomopsis fruit rot resistance in eggplant advanced lines.” Journal of Bioscience and Agriculture Research, 06, no. 01 (2016): 530-540.
Bilkiss, M., Akhter, R., Islam, M. M., Khalil, M. I. & Meah, M. B. (2016). RAPD analysis of phomopsis fruit rot resistance in eggplant advanced lines. Journal of Bioscience and Agriculture Research, 06(01), 530-540.
MLA (Modern Language Association)
Bilkiss, M., Akhter, R., Islam, M. M., Khalil, M. I. & Meah, M. B. “RAPD analysis of phomopsis fruit rot resistance in eggplant advanced lines.” Journal of Bioscience and Agriculture Research, 06.01 (2016): 530-540.
Chicago/Turabian
Bilkiss, M., Akhter, R., Islam, M. M., Khalil, M. I. & Meah, M. B. “RAPD analysis of phomopsis fruit rot resistance in eggplant advanced lines.” Journal of Bioscience and Agriculture Research, 06, no. 01 (2016): 530-540.
- Ahmed, Z. U., Begum, S. N. & Begum, S. (1998). Two new mungbean varieties- Binamoog-3 and Binamoog-4 for cultivation in Bangladesh. Bangladesh J. Nuclear Agric., 14, 53-58.
- Anonymous, (2005). Fertilizer Recommendation Guide, Bangladesh Agricultural Research Council, Farmgate, Dhaka. p.108.
- Clark, A. G. & Langigan C. M. S. (1993). Prospects for estimating nucleotide divergence with RAPDs. Mol. Evol., 10, 1096-1111.
- Das, B. H. (1998). Studies on Phomopsis fruit rot of brinjal. An M.S. thesis submitted to the Department of Plant Pathology. Bangladesh Agricultural University, Mymensingh. pp. 29-64.
- Fukuan, Z., Gao, X. H., Cheng, G. H., Fran, S. X. & Ying, Y. (2003). RAPD analysis and identification of chilling resistance of cellular variant of the eggplant. Acta Agric. Boreali Sinica., 18, 17-19.
- Islam, S. K., Sitansu, P. & Pan, S. (1990). Efficacy of humidity and temperature on Phomopsis fruit rot of brinjal (Solanum melongena). Environment and Ecology, 8(4), 1309-1310.
- Islam, M. M. (2006). Molecular characterization of Phomopsis vexans and Solanum melongena and transfer of a Phomopsis resistance trait to cultivar dohazari. . A Ph.D. thesis, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202. pp.160.
- Kabir, M. M. (2007). Molecular Characterization of F3 offspring of eggplant crosses for resistance to phomopsis blight and Fruit rot. An M.S. thesis submitted to the Dept. of Plant Pathology. Bangladesh Agricultural University, Mymensingh. pp. 100-102.
- Kalda, T. S., Swarup, V. and Choudhury, B. (1977). Resistance to Phomopsis blight in eggplant. Vegetable Sci. India., 4(2), 90-101.
- Karihaloo, J. I., Brauner, S. & Gottlieb, L. D. (1995). Random amplified polymorphic DNA variation in the eggplant, Solanum melongena L. (Solanaceae). Theor. Appl. Genet., 90, 767-770. http://dx.doi.org/10.1007/bf00222010
- Khalil, M. I., Meah, M. B., Islam, M. M. (2013). Morphological and molecular characterization of eggplant lines for resistant to phomopsis blight and fruit rot. Int.J. Agril. Res. Innov. & Tech., 3(1), 35-46. http://dx.doi.org/10.3329/ijarit.v3i1.16053
- Khan, N. U. (1999). Studies on epidemiology, seed-borne nature and management of Phomopsis fruit rot of brinjal. M.S. thesis. Dept. of Plant Pathology, Bangladesh Agricultural University, Mymensingh. p. 134.
- Ko, H. L., Cowan, D. C., Henry, R. J., Graham, G. C., Blakeney, A. B. & Lewin, L. G. (1994). RAPD analysis of Australian rice (Oryza sativa L.) varieties. Euphytica. 80, 179-189. http://dx.doi.org/10.1007/BF00039649
- Laila, R., Siddiqua, M. K., Khalil, I., Robin, A. H. K. & Meah, B. (2012). Molecular characterization of Solanum melongena using RAPD marker for collar rot resistance. Int. Res. J. Appl. Life Sci., 1 (4), 38-65.
- Meah, M. B. (2007). Eggplants of Bangladesh. IPM laboratory, Dept. of Plant Pathoplogy. Bangladesh Agricultural University, Mymensingh. pp. 1-27.
- Mohanty, A. K., Kar, A. K. & Sitti, P. N. (1995). Efficacy of crude plant extracts of some selected plants in controlling brinjal blight and fruit rot pathogen, phomopsis vexans. Crop Research Hisar., 3, 447-448.
- Nei, M. (1972). Genetic distance between populations. American Naturalist, 106, 283-292. http://dx.doi.org/10.1086/282771
- Rahman, M., Meah, M. B., Begum, S.N. & Ullah, C. (2010). Molecular characterization of F3, F4 and F5 population of eggplant for resistance to Phomopsis vexans. Bangladesh J. Crop Sci., 21( 1 & 2), 13-20
- Sharmin, D., Khalil, M. I., Begum, S. N. & Meah, M. B. (2011). Molecular characterization of eggplant crosses by using RAPD analysis. Int. J. Sustain. Crop Prod., 6(1), 22-28.
- Sivolap, Y. M., Kutsevich, L. I., Palamarchuk, A. I. & Totsky, V. N. (1997). Molecular genetic polymorphism of winter durum wheat determined by polymerase chain reaction with arbitrary primers. Russian Agril. Sci., 1, 9-13.
- Steel, R. G. D. & Torrie, J. H. (1960). Principles and procedures of statistics. McGraw Hill Book Co. Inc. New York. pp. 107-109.
- Thormann, C. E., Ferreira, M. E., Camargo, L. E. A., Tivang, J. G. & Osborn, T. C. (1994). Comparison of RFLP and RAPD markers for estimating genetic relationships within and among cruciferous species. Theor. Appl. Genet., 88, 973-980. http://dx.doi.org/10.1007/BF00220804
- Uddin, M. K. (2010). Molecular characterization of F7 eggplant lines for resistance to phomopsis fruit rot. M.S. thesis. Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh.
- Valdmar, P. C., Ruas, F., Moreira, M. P. & Paulo, M. R. (2004). Genetic diversity among maize (Zea mays L.) landraces assessed by RAPD markers. Genet mol. Biol., 27, 2-4.
- Williams, J. G. K., Kubclik, A. R., Livak, K. J., Rafalski, J. A. & Tingey, S. V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18, 6531-6535. http://dx.doi.org/10.1093/nar/18.22.6531
- Yeh, F. C., Yang R. C., Boyle, T. B. J., Ye, Z. H. & Mao, J. X. (1999). POPGENE. The user-friendly software for population genetics analysis. Molecular Biology and Biotechnology Centre, University of Albetra, Canada.
Open Access | Powered by Scribd
© Bilkiss et al. 2016. This article published by Journal BiNET is freely available for anyone to read, share, download, print, permitted for unrestricted use and build upon, provided that the original author(s) and publisher are given due credit. All Published articles are distributed under the Creative Commons Attribution 4.0 International License.