Journal of Bioscience and Agriculture Research |
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Research article:
Influence of BAP and NAA on in vitro plantlet regeneration of local and exotic banana cultivars
Md. Anwar Hossain (a), Mehede Hassan Rubel (b), Khondoker M. Nasiruddin (c) and Fatematuz Zuhura Evamoni (a)
aDept. of Biotechnology and Genetic Engineering, bDept. of Agriculture, Noakhali Science and Technology University, Sonapur, Noakhali
cDept. of Biotechnology, Bangladesh Agricultural University, Mymensingh, Bangladesh
J. bios. agric. res. | Volume 06, issue 02, pp. 553-564 | Available online: 17 January 2016
DOI: http://dx.doi.org/10.18801/jbar.060216.66
Influence of BAP and NAA on in vitro plantlet regeneration of local and exotic banana cultivars
Md. Anwar Hossain (a), Mehede Hassan Rubel (b), Khondoker M. Nasiruddin (c) and Fatematuz Zuhura Evamoni (a)
aDept. of Biotechnology and Genetic Engineering, bDept. of Agriculture, Noakhali Science and Technology University, Sonapur, Noakhali
cDept. of Biotechnology, Bangladesh Agricultural University, Mymensingh, Bangladesh
J. bios. agric. res. | Volume 06, issue 02, pp. 553-564 | Available online: 17 January 2016
DOI: http://dx.doi.org/10.18801/jbar.060216.66
| influence_of_bap_and_naa_on_in_vitro_plantlet_regeneration_of_local_and_exotic_banana_cultivars.pdf |
Title: Influence of BAP and NAA on in vitro plantlet regeneration of local and exotic banana cultivars
Abstract: The study was conducted to assess the influence of BAP and NAA on in vitro plantlet regeneration of local and exotic banana cultivars. Shoot tip of the cultivars Grand Naine, Amritasagar and Sabri were used as explants for establishment of culture. Different concentrations of BAP (1.0, 2.0, 3.0, 4.0, 5.0 and 6.0 mg/L) were used in MS medium to study their effects on shoot formation. Explants of all the cultivars produced both single and multiple shoots but rate of shoot regeneration varied among different cultivars and BAP concentrations. The highest shoot regeneration was found in all the three cultivars at 5.0 mg/L of BAP. Among the three banana cultivars Grand Naine produced the highest shoot. Shoot proliferation rate of Grand Naine, Amritasagar and Sabri was 3.50, 3.25 and 1.75 (per explant) respectively was obtained after 30 days of culture. MS medium supplemented with different concentrations of NAA (0.5, 1.0, 2.0, 4.0 and 6.0 mg/L) were used for in vitro root formation from proliferated shoots. The cultivars Grand Naine showed the best root forming performance in all concentrations of NAA. Best results of root formation were observed on MS medium supplemented with 4.0 mg/L NAA. All the plantlets regenerated in vitro from shoot tip explants of three banana cultivars survived in the field condition but Grand Naine showed the superior performance.
Keywords: BAP, NAA, plantlet, regeneration and banana cultivars
Abstract: The study was conducted to assess the influence of BAP and NAA on in vitro plantlet regeneration of local and exotic banana cultivars. Shoot tip of the cultivars Grand Naine, Amritasagar and Sabri were used as explants for establishment of culture. Different concentrations of BAP (1.0, 2.0, 3.0, 4.0, 5.0 and 6.0 mg/L) were used in MS medium to study their effects on shoot formation. Explants of all the cultivars produced both single and multiple shoots but rate of shoot regeneration varied among different cultivars and BAP concentrations. The highest shoot regeneration was found in all the three cultivars at 5.0 mg/L of BAP. Among the three banana cultivars Grand Naine produced the highest shoot. Shoot proliferation rate of Grand Naine, Amritasagar and Sabri was 3.50, 3.25 and 1.75 (per explant) respectively was obtained after 30 days of culture. MS medium supplemented with different concentrations of NAA (0.5, 1.0, 2.0, 4.0 and 6.0 mg/L) were used for in vitro root formation from proliferated shoots. The cultivars Grand Naine showed the best root forming performance in all concentrations of NAA. Best results of root formation were observed on MS medium supplemented with 4.0 mg/L NAA. All the plantlets regenerated in vitro from shoot tip explants of three banana cultivars survived in the field condition but Grand Naine showed the superior performance.
Keywords: BAP, NAA, plantlet, regeneration and banana cultivars
APA (American Psychological Association)
Hossain, M. A., Rubel, M. H., Nasiruddin, K. M. & Evamoni, F. Z. (2016). Influence of BAP and NAA on in vitro plantlet regeneration of local and exotic banana cultivars. Journal of Bioscience and Agriculture Research, 06(02), 553-564.
MLA (Modern Language Association)
Hossain, M. A., Rubel, M. H., Nasiruddin, K. M. & Evamoni, F. Z. “Influence of BAP and NAA on in vitro plantlet regeneration of local and exotic banana cultivars.” Journal of Bioscience and Agriculture Research, 06. 02 (2016): 553-564.
Chicago/Turabian
Hossain, M. A., Rubel, M. H., Nasiruddin, K. M. & Evamoni, F. Z. “Influence of BAP and NAA on in vitro plantlet regeneration of local and exotic banana cultivars.” Journal of Bioscience and Agriculture Research, 06, no. 02 (2016): 553-564.
Hossain, M. A., Rubel, M. H., Nasiruddin, K. M. & Evamoni, F. Z. (2016). Influence of BAP and NAA on in vitro plantlet regeneration of local and exotic banana cultivars. Journal of Bioscience and Agriculture Research, 06(02), 553-564.
MLA (Modern Language Association)
Hossain, M. A., Rubel, M. H., Nasiruddin, K. M. & Evamoni, F. Z. “Influence of BAP and NAA on in vitro plantlet regeneration of local and exotic banana cultivars.” Journal of Bioscience and Agriculture Research, 06. 02 (2016): 553-564.
Chicago/Turabian
Hossain, M. A., Rubel, M. H., Nasiruddin, K. M. & Evamoni, F. Z. “Influence of BAP and NAA on in vitro plantlet regeneration of local and exotic banana cultivars.” Journal of Bioscience and Agriculture Research, 06, no. 02 (2016): 553-564.
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Open Access
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