Journal of Bioscience and Agriculture Research |
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Research article:
Screening for BLB resistance in BRRI and BINA released rice germplasms using RAPD markers
P. C. Paul (a), T. Chakrobarty (b) and K. M. Nasiruddin (a)
aDept. of Biotechnology, Bangladesh Agricultural University (BAU) , Mymensing
bDept. of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensing, Bangladesh
J. bios. agric. res. | Volume 07, issue 01, pp. 608-620 | Available online: 29 February 2016
DOI: http://dx.doi.org/10.18801/jbar.070116.73
Screening for BLB resistance in BRRI and BINA released rice germplasms using RAPD markers
P. C. Paul (a), T. Chakrobarty (b) and K. M. Nasiruddin (a)
aDept. of Biotechnology, Bangladesh Agricultural University (BAU) , Mymensing
bDept. of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensing, Bangladesh
J. bios. agric. res. | Volume 07, issue 01, pp. 608-620 | Available online: 29 February 2016
DOI: http://dx.doi.org/10.18801/jbar.070116.73
| screening_for_blb_resistance_in_brri_and_bina_released_rice_germplasms_using_rapd_markers.pdf |
Title: Screening for BLB resistance in BRRI and BINA released rice germplasms using RAPD marke
Abstract: The present work was conducted to identify Random Amplified Polymorphic DNA (RAPD) markers linked to the rice Bacterial Leaf Blight (BLB) resistance gene for a marker-assisted selection program. Eight genotypes were screened by RAPD marker. These are Iratom 24, Binasail, Binadhan 4, Binadhan 5, Binadhan 6, Binadhan 7, Brridhan 29 and BR 14. Out of 12 decamer random primers, two were used to amplify genomic DNA and the primers yielded a total of 12 RAPD markers of which 2 were considered as polymorphic. The primer 70AB10C10 produced maximum number of bands (7), whereas the least number of bands (4) was generated by the primer 71AB10G11. The percentage of polymorphic loci was 36.36. For all loci, the average number of allele was 1.1818 and the average number of gene diversity was 0.0787 and their standard deviation and mean were 0.4045 and 0.1758, respectively. A dendrogram based on genetic distance using UPGMA, indicates segregation of eight germplasms of rice into two main clusters Iratom 24, Binasail and BR 14 grouped in cluster I, while Binadhan 4, Binadhan 5, Binadhan 6, Binadhan 7, Brridhan 29 in cluster II. In cluster I, Iratom 24 alone formed sub cluster I; Binasail and BR 14 formed sub cluster II. In cluster II, Binadhan 4 forms subcluster I and Binadhan 5, Binadhan 6, Binadhan 7, Brridhan 29 grouped in sub cluster II. Brridhan 29 formed alone sub sub-cluster I. Again, sub sub-cluster I; Binadhan 7 formed alone cluster and Binadhan 5, Binadhan 6 formed sub sub-cluster III.The highest genetic distance (0.2007) was observed in Binasail Vs Binadhan 5, Binasail Vs Binadhan 6, Binasail Vs Binadhan 7, Binasail Vs Brridhan 29 and BR 14 Vs Binadhan 5,BR 14 Vs Binadhan 7, BR 14 Vs Brridhan 29 germplasms pair whereas, no genetic distance was estimated in Binadhan 6 Vs Binadhan 7, Binadhan 6 Vs Brridhan 29, Binadhan 5 Vs Binadhan 7, Binadhan 5 Vs Brridhan 29. Results are discussed considering the potential utility of the RAPD markers found, for marker assisted selection in rice breeding programs aiming at developing rice varieties with durable BLB resistance based on a combination of resistance genes.
Key Words: Rice variety, Genetic relationships, BLB resistance and RAPD marker
Abstract: The present work was conducted to identify Random Amplified Polymorphic DNA (RAPD) markers linked to the rice Bacterial Leaf Blight (BLB) resistance gene for a marker-assisted selection program. Eight genotypes were screened by RAPD marker. These are Iratom 24, Binasail, Binadhan 4, Binadhan 5, Binadhan 6, Binadhan 7, Brridhan 29 and BR 14. Out of 12 decamer random primers, two were used to amplify genomic DNA and the primers yielded a total of 12 RAPD markers of which 2 were considered as polymorphic. The primer 70AB10C10 produced maximum number of bands (7), whereas the least number of bands (4) was generated by the primer 71AB10G11. The percentage of polymorphic loci was 36.36. For all loci, the average number of allele was 1.1818 and the average number of gene diversity was 0.0787 and their standard deviation and mean were 0.4045 and 0.1758, respectively. A dendrogram based on genetic distance using UPGMA, indicates segregation of eight germplasms of rice into two main clusters Iratom 24, Binasail and BR 14 grouped in cluster I, while Binadhan 4, Binadhan 5, Binadhan 6, Binadhan 7, Brridhan 29 in cluster II. In cluster I, Iratom 24 alone formed sub cluster I; Binasail and BR 14 formed sub cluster II. In cluster II, Binadhan 4 forms subcluster I and Binadhan 5, Binadhan 6, Binadhan 7, Brridhan 29 grouped in sub cluster II. Brridhan 29 formed alone sub sub-cluster I. Again, sub sub-cluster I; Binadhan 7 formed alone cluster and Binadhan 5, Binadhan 6 formed sub sub-cluster III.The highest genetic distance (0.2007) was observed in Binasail Vs Binadhan 5, Binasail Vs Binadhan 6, Binasail Vs Binadhan 7, Binasail Vs Brridhan 29 and BR 14 Vs Binadhan 5,BR 14 Vs Binadhan 7, BR 14 Vs Brridhan 29 germplasms pair whereas, no genetic distance was estimated in Binadhan 6 Vs Binadhan 7, Binadhan 6 Vs Brridhan 29, Binadhan 5 Vs Binadhan 7, Binadhan 5 Vs Brridhan 29. Results are discussed considering the potential utility of the RAPD markers found, for marker assisted selection in rice breeding programs aiming at developing rice varieties with durable BLB resistance based on a combination of resistance genes.
Key Words: Rice variety, Genetic relationships, BLB resistance and RAPD marker
APA (American Psychological Association)
Paul, P. C., Chakrobarty, T. & Nasiruddin, K. M. (2016). Screening for BLB resistance in BRRI and BINA released rice germplasms using RAPD markers. Journal of Bioscience and Agriculture Research, 07(01), 608-620.
MLA (Modern Language Association)
Paul, P. C., Chakrobarty, T. & Nasiruddin, K. M. “Black Screening for BLB resistance in BRRI and BINA released rice germplasms using RAPD markers.” Journal of Bioscience and Agriculture Research, 07.01 (2016), 608-620.
Chicago/Turabian
Paul, P. C., Chakrobarty, T. & Nasiruddin, K. M. “Screening for BLB resistance in BRRI and BINA released rice germplasms using RAPD markers.” Journal of Bioscience and Agriculture Research, 07, no. 01 (2016), 608-620.
Paul, P. C., Chakrobarty, T. & Nasiruddin, K. M. (2016). Screening for BLB resistance in BRRI and BINA released rice germplasms using RAPD markers. Journal of Bioscience and Agriculture Research, 07(01), 608-620.
MLA (Modern Language Association)
Paul, P. C., Chakrobarty, T. & Nasiruddin, K. M. “Black Screening for BLB resistance in BRRI and BINA released rice germplasms using RAPD markers.” Journal of Bioscience and Agriculture Research, 07.01 (2016), 608-620.
Chicago/Turabian
Paul, P. C., Chakrobarty, T. & Nasiruddin, K. M. “Screening for BLB resistance in BRRI and BINA released rice germplasms using RAPD markers.” Journal of Bioscience and Agriculture Research, 07, no. 01 (2016), 608-620.
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