Journal of Bioscience and Agriculture Research |
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Research article:
In vitro regeneration potentiality of some Brassica genotypes
S. C. Das (a), L. Hassan (a), M. A. Quddus (a) and B. P. Ray (b)
aDept. of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh
bBiotechnology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh
J. bios. agric. res. | Volume 07, Issue 02, pp. 638-646 | Available online: 31 March 2016
DOI: http://dx.doi.org/10.18801/jbar.070216.76
In vitro regeneration potentiality of some Brassica genotypes
S. C. Das (a), L. Hassan (a), M. A. Quddus (a) and B. P. Ray (b)
aDept. of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh
bBiotechnology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh
J. bios. agric. res. | Volume 07, Issue 02, pp. 638-646 | Available online: 31 March 2016
DOI: http://dx.doi.org/10.18801/jbar.070216.76
| 76_in_vitro_regeneration__potentiality_of_some_brassica_genotypes.pdf |
Title: In vitro regeneration potentiality of some Brassica genotypes
Abstract: Ten genotypes of Brassica, from three different groups of species, namely Tori-7, Kallyania, Sampad, Sonali (each from Brassica campestris), BARI sarisa-7, BARI sarisa-8 and MM-20-3 (each from Brassica napus), Rai-5, Shambal and Daulat (each from Brassica juncea) were selected to observe their in vitro regeneration potentiality using stem segment as the explants. Investigations on regeneration of these ten genotypes were accomplished with callus induction, maintenance of calli, organogenesis and finally plantlet regeneration and subsequent their establishment in the field condition. Among the varieties, BARI sarisa-8 showed the highest response in all aspects. Callus induction was highest in BARI sarisa-8 (99.60%) and lowest in Sonali (92.40%). Time required for shoot initiation was minimum in BARI sarisa-8 (21.12days) while it was maximum in Kallyania (25.12days). Number of shoots per callus was highest in BARI sarisa-8 (2.502) and lowest in MM-20-3 (1.208). Number of rooted shoots was highest in BARI sarisa-8 (55.56%). Survival rate of regenerated plantlets was highest in BARI sarisa-8 (80.00% in pot and 75.00% in soil) while the same was lowest in Kallyania (61.11% in pot) and in BARI sarisa-7 (30.00% in soil).
Key Words: In vitro regeneration, Brassica, Hypocotyl segment, Genotypes and Potentiality
Abstract: Ten genotypes of Brassica, from three different groups of species, namely Tori-7, Kallyania, Sampad, Sonali (each from Brassica campestris), BARI sarisa-7, BARI sarisa-8 and MM-20-3 (each from Brassica napus), Rai-5, Shambal and Daulat (each from Brassica juncea) were selected to observe their in vitro regeneration potentiality using stem segment as the explants. Investigations on regeneration of these ten genotypes were accomplished with callus induction, maintenance of calli, organogenesis and finally plantlet regeneration and subsequent their establishment in the field condition. Among the varieties, BARI sarisa-8 showed the highest response in all aspects. Callus induction was highest in BARI sarisa-8 (99.60%) and lowest in Sonali (92.40%). Time required for shoot initiation was minimum in BARI sarisa-8 (21.12days) while it was maximum in Kallyania (25.12days). Number of shoots per callus was highest in BARI sarisa-8 (2.502) and lowest in MM-20-3 (1.208). Number of rooted shoots was highest in BARI sarisa-8 (55.56%). Survival rate of regenerated plantlets was highest in BARI sarisa-8 (80.00% in pot and 75.00% in soil) while the same was lowest in Kallyania (61.11% in pot) and in BARI sarisa-7 (30.00% in soil).
Key Words: In vitro regeneration, Brassica, Hypocotyl segment, Genotypes and Potentiality
APA (American Psychological Association)
Das, S. C., Hassan, L., Quddus, M. A. & Ray, B. P. (2016). In vitro regeneration potentiality of some Brassica genotypes. Journal of Bioscience and Agriculture Research, 07(02), 638-646.
MLA (Modern Language Association)
Das, S. C., Hassan, L., Quddus, M. A. & Ray, B. P. "In vitro regeneration potentiality of some Brassica genotypes." Journal of Bioscience and Agriculture Research, 07.02 (2016), 638-646.
Chicago/Turabian
Das, S. C., Hassan, L., Quddus, M. A. & Ray, B. P. In vitro regeneration potentiality of some Brassica genotypes. Journal of Bioscience and Agriculture Research, 07, no. 02 (2016), 638-646.
Das, S. C., Hassan, L., Quddus, M. A. & Ray, B. P. (2016). In vitro regeneration potentiality of some Brassica genotypes. Journal of Bioscience and Agriculture Research, 07(02), 638-646.
MLA (Modern Language Association)
Das, S. C., Hassan, L., Quddus, M. A. & Ray, B. P. "In vitro regeneration potentiality of some Brassica genotypes." Journal of Bioscience and Agriculture Research, 07.02 (2016), 638-646.
Chicago/Turabian
Das, S. C., Hassan, L., Quddus, M. A. & Ray, B. P. In vitro regeneration potentiality of some Brassica genotypes. Journal of Bioscience and Agriculture Research, 07, no. 02 (2016), 638-646.
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