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Journal of Bioscience and Agriculture Research 
Research article: 
Agrobacterium-mediated genetic transformation in Brassica species
 
S. C. Das, L. Hassan and M. A. Quddus
 
Dept. of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

​J. bios. agric. res. | Volume 07, Issue 02, pp. 647-658 | Available online: 31 March 2016
DOI: http://dx.doi.org/10.18801/jbar.070216.77
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Title: Agrobacterium-mediated genetic transformation in Brassica species
​Abstract: An efficient and reproducible protocol for the production of transgenic Brassica plants was developed by inoculating hypocotyl explants with Agrobacterium tumefaciens strain LBA4404 carrying a binary vector pBI121, which contains selectable marker gene nptII conferring resistance to kanamycin and the GUS reporter gene. The transformation experiment was performed by optimizing two important parameters: preculture period and co-cultivation period. Infection was most effective when explants were precultured for three days (68.75% GUS positive) and co-cultivated for three days (82.92% GUS positive) with Agrobacterium. Among the varieties, BARI sarisa-8 showed the highest response to GUS assay (64.38% GUS positive). Callus induction was highest in Rai-5 (10%) and three day period of preculture and co-cultivation (12.33 and 13.33%, respectively). Transformation percentage was also highest in Rai-5 (4.75%), and three day period of preculture and co-cultivation (7.50 and 5.42%, respectively). Highest percentage (33.33) of root induction from transgenic shoots was observed in ½ MS + 0.5 mgL-1 NAA + 50 mgL-1 kanamycin + 50 mgL-1 cefotaxime. Among the varieties, BARI sarisa-8 produced the highest percentage (37.78) of rooted shoots.
Key Words: Agrobacterium tumefaciens, Brassica, Co-cultivation, Preculture and Transformation
​APA (American Psychological Association)
Das, S. C., Hassan, L. & Quddus, M. A. (2016). Agrobacterium-mediated genetic transformation in Brassica species. Journal of Bioscience and Agriculture Research, 07(02), 647-658.
 
MLA (Modern Language Association) 
Das, S. C., Hassan, L. & Quddus, M. A. "Agrobacterium-mediated genetic transformation in Brassica species." Journal of Bioscience and Agriculture Research, 07.02 (2016), 647-658.

Chicago/Turabian
Das, S. C., Hassan, L. & Quddus, M. A. Agrobacterium-mediated genetic transformation in Brassica species. Journal of Bioscience and Agriculture Research, 07, no. 02 (2016), 647-658.
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