Journal of Bioscience and Agriculture Research |
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Research article:
Herbal extract (Ficus racemosa) restores the damages of diabetic pancreas both at gross and cellular levels: an in vivo study in mice
Md. Mahmudul Amin (b), Sonali Bhakta (b) , Md. Abdullah-Al-Mahmud (a) , Md. Abdul Awal (c) and Shonkor Kumar Das (b)
aDept. of Anatomy and Histology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur
bBioresearch Laboratory (Cancer and Herbal Research Center)
b,cDept. of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh, Bangladesh
J. bios. agric. res. | Volume 08, Issue 01, pp. 695-702 | Available online: 26 April 2016
DOI: http://dx.doi.org/10.18801/jbar.080116.82
Herbal extract (Ficus racemosa) restores the damages of diabetic pancreas both at gross and cellular levels: an in vivo study in mice
Md. Mahmudul Amin (b), Sonali Bhakta (b) , Md. Abdullah-Al-Mahmud (a) , Md. Abdul Awal (c) and Shonkor Kumar Das (b)
aDept. of Anatomy and Histology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur
bBioresearch Laboratory (Cancer and Herbal Research Center)
b,cDept. of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh, Bangladesh
J. bios. agric. res. | Volume 08, Issue 01, pp. 695-702 | Available online: 26 April 2016
DOI: http://dx.doi.org/10.18801/jbar.080116.82
| 82_jbar_herbal_extract_restores__the_damages_of_diabetic_pancreas_both_at_gross_and_cellular_levels_an_in__vivo_study_in_mice.pdf |
Title: Herbal extract (Ficus racemosa) restores the damages of diabetic pancreas both at gross and cellular levels: an in vivo study in mice
Abstract: An in vivo investigation on the gross and cellular changes of the pancreas of Swiss albino mice treated with Ficus racemosa extract was carried out at the Bioresearch Laboratory, Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh. Twenty (20) Swiss albino mice (Mus musculus, age: 4-5 weeks, weight 25-28g, purchased from ICDDR,B) were used for this experiment and were grouped (each group has 5 mice) as the control group (C), diabetic control group (DC), glibenclamide treated group (GL) and Ficus racemosa extract treated group (FR) and provided with feed (standard mice pellets purchased from ICDDR,B) and water ad libitum. Diabetes mellitus was induced by single intra-peritoneal injection of freshly prepared solution of alloxan monohydrate (150mg/kg body weight) dissolved in physiological saline in overnight fasted mice. The ethanolic extracts of Ficus racemosa fruit (250mg/kg body weight) was administered orally once daily for 30 days in comparison to a standard antidiabetic drug, glibenclamide (600μg/kg body weight). The uniformity of all the management practices was also maintained. The total experimental tenure was 30 days. At the end of the experiment, mice were ethically sacrificed and necessary samples were collected from the pancreas and were preserved, processed for slide preparation and stained (H & E stain) for histo-morphological investigation. Pancreas of the normal control group showed no morphological changes, whereas, both the colour and shape were significantly changed in the diabetic control group. The alteration was effectively restored in the standard anti-diabetic drug (glibenclamide) treated group as well as the Ficus racemosa extract treated group. Histologically, necrosis in the islets and focal acinar damages, found in the diabetic pancreas were restored to normal in the Ficus racemosa extract treated groups, and were significantly related to that of the effect produced by standard antidiabetic drug, glibenclamide. Therefore, it is assumed that the Ficus racemosa extract might be used as an anti-diabetic drug or as an adjuvant in the diabetic therapy. Results might aid to explore a frontier, inexpensive, safe and effective anti-diabetic drug in future.
Key Words: Ficus racemosa, Diabetes, Pancreas, Histo-morphology and Swiss albino mice
Abstract: An in vivo investigation on the gross and cellular changes of the pancreas of Swiss albino mice treated with Ficus racemosa extract was carried out at the Bioresearch Laboratory, Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh. Twenty (20) Swiss albino mice (Mus musculus, age: 4-5 weeks, weight 25-28g, purchased from ICDDR,B) were used for this experiment and were grouped (each group has 5 mice) as the control group (C), diabetic control group (DC), glibenclamide treated group (GL) and Ficus racemosa extract treated group (FR) and provided with feed (standard mice pellets purchased from ICDDR,B) and water ad libitum. Diabetes mellitus was induced by single intra-peritoneal injection of freshly prepared solution of alloxan monohydrate (150mg/kg body weight) dissolved in physiological saline in overnight fasted mice. The ethanolic extracts of Ficus racemosa fruit (250mg/kg body weight) was administered orally once daily for 30 days in comparison to a standard antidiabetic drug, glibenclamide (600μg/kg body weight). The uniformity of all the management practices was also maintained. The total experimental tenure was 30 days. At the end of the experiment, mice were ethically sacrificed and necessary samples were collected from the pancreas and were preserved, processed for slide preparation and stained (H & E stain) for histo-morphological investigation. Pancreas of the normal control group showed no morphological changes, whereas, both the colour and shape were significantly changed in the diabetic control group. The alteration was effectively restored in the standard anti-diabetic drug (glibenclamide) treated group as well as the Ficus racemosa extract treated group. Histologically, necrosis in the islets and focal acinar damages, found in the diabetic pancreas were restored to normal in the Ficus racemosa extract treated groups, and were significantly related to that of the effect produced by standard antidiabetic drug, glibenclamide. Therefore, it is assumed that the Ficus racemosa extract might be used as an anti-diabetic drug or as an adjuvant in the diabetic therapy. Results might aid to explore a frontier, inexpensive, safe and effective anti-diabetic drug in future.
Key Words: Ficus racemosa, Diabetes, Pancreas, Histo-morphology and Swiss albino mice
APA (American Psychological Association)
Amin, M. A., Bhakta, S., Abdullah-Al-Mahmud, M., Awal, M. A. & Das, S. K. (2016). Herbal extract (Ficus racemosa) restores the damages of diabetic pancreas both at gross and cellular levels: An in vivo study in mice. Journal of Bioscience and Agriculture Research, 08(01), 695-702.
MLA (Modern Language Association)
Amin, M. A., Bhakta, S., Abdullah-Al-Mahmud, M., Awal, M. A. & Das, S. K. "Herbal extract (Ficus racemosa) restores the damages of diabetic pancreas both at gross and cellular levels: An in vivo study in mice." Journal of Bioscience and Agriculture Research, 08.01 (2016), 695-702.
Chicago/Turabian
Amin, M. A., Bhakta, S., Abdullah-Al-Mahmud, M., Awal, M. A. & Das, S. K. Herbal extract (Ficus racemosa) restores the damages of diabetic pancreas both at gross and cellular levels: An in vivo study in mice. Journal of Bioscience and Agriculture Research, 08, no. 01 (2016), 695-702.
Amin, M. A., Bhakta, S., Abdullah-Al-Mahmud, M., Awal, M. A. & Das, S. K. (2016). Herbal extract (Ficus racemosa) restores the damages of diabetic pancreas both at gross and cellular levels: An in vivo study in mice. Journal of Bioscience and Agriculture Research, 08(01), 695-702.
MLA (Modern Language Association)
Amin, M. A., Bhakta, S., Abdullah-Al-Mahmud, M., Awal, M. A. & Das, S. K. "Herbal extract (Ficus racemosa) restores the damages of diabetic pancreas both at gross and cellular levels: An in vivo study in mice." Journal of Bioscience and Agriculture Research, 08.01 (2016), 695-702.
Chicago/Turabian
Amin, M. A., Bhakta, S., Abdullah-Al-Mahmud, M., Awal, M. A. & Das, S. K. Herbal extract (Ficus racemosa) restores the damages of diabetic pancreas both at gross and cellular levels: An in vivo study in mice. Journal of Bioscience and Agriculture Research, 08, no. 01 (2016), 695-702.
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