Journal of Bioscience and Agriculture Research |
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Research Article:
Callus induction and efficient plant regeneration in Cucumber (Cucumis sativus L.)
Rubaiya Jesmin and M Abdul Khaleque Mian
Dept. of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur-1706, Bangladesh
J. bios. agric. res. | Volume 09, Issue 02, pp. 796-803 | Available online: 25 July 2016
DOI: http://dx.doi.org/10.18801/jbar.090216.96
Callus induction and efficient plant regeneration in Cucumber (Cucumis sativus L.)
Rubaiya Jesmin and M Abdul Khaleque Mian
Dept. of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur-1706, Bangladesh
J. bios. agric. res. | Volume 09, Issue 02, pp. 796-803 | Available online: 25 July 2016
DOI: http://dx.doi.org/10.18801/jbar.090216.96
| 96.09.02.16_callus_induction_and_efficient_plant_regeneration_in_cucumber.pdf |
Title: Callus induction and efficient plant regeneration in Cucumber (Cucumis sativus L.)
Abstract: A reliable and reproducible protocol is established to get healthy and wellformed callus from juvenile explants of cucumber. The sterilized seeds of cucumber cultivar were cultured on MS basal medium (Murashige and Skoog, 1962). The seeds germinated after 7 days of culture with 24 hours dark photoperiod. Explants from germinated seedlings were cultured on MS medium supplemented with individual treatments of different auxins (2,4-dichloro-phenoxyacetic acid (2,4-D), α naphthalene acetic acid (NAA)) or cytokinins (benzyl aminopurine (BAP)). Plant parts such as leaves, stems and cotyledons were used as source of explants. Callus were initiated from leaves, stems and cotyledons after 4 weeks of culture. The optimum medium for callus induction from leave, stem and cotyledon explants was MS medium supplemented with 0.5 mg/L BAP added with 1.0 mg/L NAA. The highest percentage of callus was obtained from stem explants (89.0 ± 0.75 %) followed by leave (79.05 ± 3.28%) and cotyledon (74.43 ± 1.30 %) explants. Maximum callus induction in stems (73.05 ± 2.1%) was obtained in 1.mg/l concentration of BAP. Incorporating 2,4-D in the callus induction media promoted slow callus growth and low quality callus compared to that produced on media containing NAA and BAP. Callus induced on media containing 2,4-D was friable and yellow in color. This protocol can promote the application of tissue culture technology to facilitate the genetic transformation of this species.
Key Words: Cotyledon, Naphatalene acetic acid, Benzylaminopurine, propagation and Explants
Abstract: A reliable and reproducible protocol is established to get healthy and wellformed callus from juvenile explants of cucumber. The sterilized seeds of cucumber cultivar were cultured on MS basal medium (Murashige and Skoog, 1962). The seeds germinated after 7 days of culture with 24 hours dark photoperiod. Explants from germinated seedlings were cultured on MS medium supplemented with individual treatments of different auxins (2,4-dichloro-phenoxyacetic acid (2,4-D), α naphthalene acetic acid (NAA)) or cytokinins (benzyl aminopurine (BAP)). Plant parts such as leaves, stems and cotyledons were used as source of explants. Callus were initiated from leaves, stems and cotyledons after 4 weeks of culture. The optimum medium for callus induction from leave, stem and cotyledon explants was MS medium supplemented with 0.5 mg/L BAP added with 1.0 mg/L NAA. The highest percentage of callus was obtained from stem explants (89.0 ± 0.75 %) followed by leave (79.05 ± 3.28%) and cotyledon (74.43 ± 1.30 %) explants. Maximum callus induction in stems (73.05 ± 2.1%) was obtained in 1.mg/l concentration of BAP. Incorporating 2,4-D in the callus induction media promoted slow callus growth and low quality callus compared to that produced on media containing NAA and BAP. Callus induced on media containing 2,4-D was friable and yellow in color. This protocol can promote the application of tissue culture technology to facilitate the genetic transformation of this species.
Key Words: Cotyledon, Naphatalene acetic acid, Benzylaminopurine, propagation and Explants
APA (American Psychological Association)
Jesmin, R. & Mian, M. A. K. (2016). Callus induction and efficient plant regeneration in Cucumber (Cucumis sativus L.). Journal of Bioscience and Agriculture Research, 09(02), 796-803.
MLA (Modern Language Association)
Jesmin, R. & Mian, M. A. K. "Callus induction and efficient plant regeneration in Cucumber (Cucumis sativus L.)." Journal of Bioscience and Agriculture Research, 09.02 (2016), 796-803.
Chicago/Turabian
Jesmin, R. & Mian, M. A. K. Callus induction and efficient plant regeneration in Cucumber (Cucumis sativus L.). Journal of Bioscience and Agriculture Research, 09, no. 02 (2016), 796-803.
Jesmin, R. & Mian, M. A. K. (2016). Callus induction and efficient plant regeneration in Cucumber (Cucumis sativus L.). Journal of Bioscience and Agriculture Research, 09(02), 796-803.
MLA (Modern Language Association)
Jesmin, R. & Mian, M. A. K. "Callus induction and efficient plant regeneration in Cucumber (Cucumis sativus L.)." Journal of Bioscience and Agriculture Research, 09.02 (2016), 796-803.
Chicago/Turabian
Jesmin, R. & Mian, M. A. K. Callus induction and efficient plant regeneration in Cucumber (Cucumis sativus L.). Journal of Bioscience and Agriculture Research, 09, no. 02 (2016), 796-803.
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