Journal of Bioscience and Agriculture Research |
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RESEARCH ARTICLE:
In vitro PLBs organogenesis of Phalaenopsis using different concentrations of HA9 and HA12 combination
Hasan Mehraj (1,2) and Kazuhiko Shimasaki (2)
1The United Graduate School of Agricultural Sciences, Ehime University, Ehime 790-8556, Japan
2Faculty of Agriculture, Kochi University, B200 Monobe, Nankoku, Kochi 783-8520, Japan
J. bios. agric. res. | Volume 12, Issue 02, pp. 1036-1040 | Available online: 14 March 2017.
Crossref: https://doi.org/10.18801/jbar.120217.126
In vitro PLBs organogenesis of Phalaenopsis using different concentrations of HA9 and HA12 combination
Hasan Mehraj (1,2) and Kazuhiko Shimasaki (2)
1The United Graduate School of Agricultural Sciences, Ehime University, Ehime 790-8556, Japan
2Faculty of Agriculture, Kochi University, B200 Monobe, Nankoku, Kochi 783-8520, Japan
J. bios. agric. res. | Volume 12, Issue 02, pp. 1036-1040 | Available online: 14 March 2017.
Crossref: https://doi.org/10.18801/jbar.120217.126
FULL TEXT PDF:
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Title: In vitro PLBs organogenesis of Phalaenopsis using different concentrations of HA9 and HA12 combination
Abstract: The organogenesis of PLBs of Phalaenopsis ‘Fmk02010’ was studied to find out the effective treatment combination of HA9 and HA12. We used five different concentrations of HA9 and HA12 combinations and those were C0: 0, C1: 0.01, C2: 0.10, C3: 1.00 and C4: 10.00 mgL-1. Explants were cultured in modified MS medium for 42 days. The highest numbers of PLBs (18.4/explant) were found from C2 which was statistically identical with C1 (15.20/explant). The PLBs formation rate was hundred percent in C1, C2 and C3. Numbers of PLBs were reduced in the higher concentrations (C3 and C4) which were lower than that of control while PLBs formation rate was reduced only in C4 (60.0%) than the control (86.7%). Numbers of shoots were also decreased with the increase of concentration. Maximum numbers of roots were found from C1 (1.0/explant) while rest of the treatments showed lower number of roots than that of control. The highest fresh weight was found in C2 (0.307 g) and lowest was in C4 (0.228 g); C4 was statistically identical with C3 (0.229 g).
Key Words: Orchid, Hyaluronic Acid, Protocorm-like body and Formation rate
Abstract: The organogenesis of PLBs of Phalaenopsis ‘Fmk02010’ was studied to find out the effective treatment combination of HA9 and HA12. We used five different concentrations of HA9 and HA12 combinations and those were C0: 0, C1: 0.01, C2: 0.10, C3: 1.00 and C4: 10.00 mgL-1. Explants were cultured in modified MS medium for 42 days. The highest numbers of PLBs (18.4/explant) were found from C2 which was statistically identical with C1 (15.20/explant). The PLBs formation rate was hundred percent in C1, C2 and C3. Numbers of PLBs were reduced in the higher concentrations (C3 and C4) which were lower than that of control while PLBs formation rate was reduced only in C4 (60.0%) than the control (86.7%). Numbers of shoots were also decreased with the increase of concentration. Maximum numbers of roots were found from C1 (1.0/explant) while rest of the treatments showed lower number of roots than that of control. The highest fresh weight was found in C2 (0.307 g) and lowest was in C4 (0.228 g); C4 was statistically identical with C3 (0.229 g).
Key Words: Orchid, Hyaluronic Acid, Protocorm-like body and Formation rate
HOW TO CITE THIS ARTICLE?
APA (American Psychological Association)
Mehraj, H. and Shimasaki, K. (2017). In vitro PLBs organogenesis of Phalaenopsis using different concentrations of HA9 and HA12 combination. Journal of Bioscience and Agriculture Research,12(02), 1036-1040.
MLA (Modern Language Association)
Mehraj, H. and Shimasaki, K. “In vitro PLBs organogenesis of Phalaenopsis using different concentrations of HA9 and HA12 combination”. Journal of Bioscience and Agriculture Research, 12.02(2017), 1036-1040.
Chicago and orTurabian
Mehraj, H. and Shimasaki, K. “In vitro PLBs organogenesis of Phalaenopsis using different concentrations of HA9 and HA12 combination”. Journal of Bioscience and Agriculture Research, 12. no. 02(2017), 1036-1040.
APA (American Psychological Association)
Mehraj, H. and Shimasaki, K. (2017). In vitro PLBs organogenesis of Phalaenopsis using different concentrations of HA9 and HA12 combination. Journal of Bioscience and Agriculture Research,12(02), 1036-1040.
MLA (Modern Language Association)
Mehraj, H. and Shimasaki, K. “In vitro PLBs organogenesis of Phalaenopsis using different concentrations of HA9 and HA12 combination”. Journal of Bioscience and Agriculture Research, 12.02(2017), 1036-1040.
Chicago and orTurabian
Mehraj, H. and Shimasaki, K. “In vitro PLBs organogenesis of Phalaenopsis using different concentrations of HA9 and HA12 combination”. Journal of Bioscience and Agriculture Research, 12. no. 02(2017), 1036-1040.
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