RESEARCH ARTICLE:
Adaptability of dekoko (Pisum sativum var. abyssinicum) seedlings to salinity stress in vitro culture conditions
Berhane Gebreslassie Gebreegziabher and Berhanu Abraha Tsegay
Dept. of Biology, College of Science, Bahir Dar University, Ethiopia.
J. bios. agric. res. | Volume 12, Issue 02, pp. 1054-1063 | Available online: 14 March 2017.
Crossref: https://doi.org/10.18801/jbar.120217.129
Adaptability of dekoko (Pisum sativum var. abyssinicum) seedlings to salinity stress in vitro culture conditions
Berhane Gebreslassie Gebreegziabher and Berhanu Abraha Tsegay
Dept. of Biology, College of Science, Bahir Dar University, Ethiopia.
J. bios. agric. res. | Volume 12, Issue 02, pp. 1054-1063 | Available online: 14 March 2017.
Crossref: https://doi.org/10.18801/jbar.120217.129
FULL TEXT PDF:
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Title: Adaptability of dekoko (Pisum sativum var. abyssinicum) seedlings to salinity stress in vitro culture conditions
Abstract: Dekoko (Pisum sativum var. abyssinicum) is one of the most important food legumes grown in south Tigray and north Wollo, northern Ethiopia. It is one among the most important food legumes in terms of price and protein content. It grows mixed and alone with many cereal crops growing in north Ethiopia. This study was conducted with the objective of selecting adaptable and relatively high yielding P. sativum var. abyssinicum collections under different salt (NaCl) concentrations at laboratory conditions. Seeds for 30 collections were obtained from nine districts; two regional states of north Ethiopia with different attitudinal ranges 1300 m.a.s.l being the lowest and 3148 m.a.s.l the highest. Of the 30 on farm and wire house tested local collections, six vigorously growing local collections, three from Ofla (T-001/08OF, T-002/08OF and, T-003/08OF), one from Sirinka (TA-026/15Sr), one from Emba-Alaje (T-025/15E/A), and one from Endamohoni (T-023/08MW) were selected four this study; for salt stress resistances in controlled condition by priming in four salt treatment levels (5 dS/m, 7 dS/m, 9 dS/m and 15 dS/m). Distilled water (0 dS/m) was used as control. Fifty (50) surface sterilized seeds per petri dish were sown for the four salt treatments and the control. Collections T-001/08 from Ofla and T-023/08 from Endamohoni showed good growth performance at 5 dS/m. However, TA-026/15Sr from Sirinka and T-025/15E/A from Betmara (Emba-Alaje) respond positively upto 7dS/m. At higher salinity level growth features decreased with increasing salinity stress. But, T-023/08MW, T-001/08OF, TA-026/15Sr followed by T-025/15E/A from lower to the higher resistances respectively could withstand lower to medium concentrations of salinity as compared to the other collections.
Key Words: Pisum sativum, Northern Ethiopia, High yielding, Resistance and Salinity level
Abstract: Dekoko (Pisum sativum var. abyssinicum) is one of the most important food legumes grown in south Tigray and north Wollo, northern Ethiopia. It is one among the most important food legumes in terms of price and protein content. It grows mixed and alone with many cereal crops growing in north Ethiopia. This study was conducted with the objective of selecting adaptable and relatively high yielding P. sativum var. abyssinicum collections under different salt (NaCl) concentrations at laboratory conditions. Seeds for 30 collections were obtained from nine districts; two regional states of north Ethiopia with different attitudinal ranges 1300 m.a.s.l being the lowest and 3148 m.a.s.l the highest. Of the 30 on farm and wire house tested local collections, six vigorously growing local collections, three from Ofla (T-001/08OF, T-002/08OF and, T-003/08OF), one from Sirinka (TA-026/15Sr), one from Emba-Alaje (T-025/15E/A), and one from Endamohoni (T-023/08MW) were selected four this study; for salt stress resistances in controlled condition by priming in four salt treatment levels (5 dS/m, 7 dS/m, 9 dS/m and 15 dS/m). Distilled water (0 dS/m) was used as control. Fifty (50) surface sterilized seeds per petri dish were sown for the four salt treatments and the control. Collections T-001/08 from Ofla and T-023/08 from Endamohoni showed good growth performance at 5 dS/m. However, TA-026/15Sr from Sirinka and T-025/15E/A from Betmara (Emba-Alaje) respond positively upto 7dS/m. At higher salinity level growth features decreased with increasing salinity stress. But, T-023/08MW, T-001/08OF, TA-026/15Sr followed by T-025/15E/A from lower to the higher resistances respectively could withstand lower to medium concentrations of salinity as compared to the other collections.
Key Words: Pisum sativum, Northern Ethiopia, High yielding, Resistance and Salinity level
HOW TO CITE THIS ARTICLE?
APA (American Psychological Association)
Gebreegziabher, B. G. and Tsegay, B. A. (2017). Adaptability of dekoko (Pisum sativum var. abyssinicum) seedlings to salinity stress in vitro culture conditions. Journal of Bioscience and Agriculture Research,12(02), 1054-1063.
MLA (Modern Language Association)
Gebreegziabher, B. G. and Tsegay, B. A. “Adaptability of dekoko (Pisum sativum var. abyssinicum) seedlings to salinity stress in vitro culture conditions”. Journal of Bioscience and Agriculture Research, 12.02(2017), 1054-1063.
Chicago and orTurabian
Gebreegziabher, B. G. and Tsegay, B. A. “Adaptability of dekoko (Pisum sativum var. abyssinicum) seedlings to salinity stress in vitro culture conditions”. Journal of Bioscience and Agriculture Research, 12. no. 02(2017), 1054-1063.
APA (American Psychological Association)
Gebreegziabher, B. G. and Tsegay, B. A. (2017). Adaptability of dekoko (Pisum sativum var. abyssinicum) seedlings to salinity stress in vitro culture conditions. Journal of Bioscience and Agriculture Research,12(02), 1054-1063.
MLA (Modern Language Association)
Gebreegziabher, B. G. and Tsegay, B. A. “Adaptability of dekoko (Pisum sativum var. abyssinicum) seedlings to salinity stress in vitro culture conditions”. Journal of Bioscience and Agriculture Research, 12.02(2017), 1054-1063.
Chicago and orTurabian
Gebreegziabher, B. G. and Tsegay, B. A. “Adaptability of dekoko (Pisum sativum var. abyssinicum) seedlings to salinity stress in vitro culture conditions”. Journal of Bioscience and Agriculture Research, 12. no. 02(2017), 1054-1063.
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