Journal of Bioscience and Agriculture Research |
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RESEARCH ARTICLE:
In vitro antioxidant activity of Anaphalis lawii (Hook. f) Gamble and Helichrysum buddleioides DC - a comparative study
K. H. Sandeepa (1), T. S. Harsha (1), M. Prashanth (1), T. R. Prashith Kekuda (2) and H. L. Raghavendra (3)
1Dept. of Biochemistry, The Oxford College of Science, Bengaluru, Karnataka, India
2Dept. of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India
3School of Medicine, Wollega University, Post Box No: 395, Nekemte, Ethiopia
J. bios. agric. res. | Volume 12, Issue 02, pp. 1064-1073 | Available online: 14 March 2017.
Crossref: https://doi.org/10.18801/jbar.120217.130
In vitro antioxidant activity of Anaphalis lawii (Hook. f) Gamble and Helichrysum buddleioides DC - a comparative study
K. H. Sandeepa (1), T. S. Harsha (1), M. Prashanth (1), T. R. Prashith Kekuda (2) and H. L. Raghavendra (3)
1Dept. of Biochemistry, The Oxford College of Science, Bengaluru, Karnataka, India
2Dept. of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India
3School of Medicine, Wollega University, Post Box No: 395, Nekemte, Ethiopia
J. bios. agric. res. | Volume 12, Issue 02, pp. 1064-1073 | Available online: 14 March 2017.
Crossref: https://doi.org/10.18801/jbar.120217.130
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Title: In vitro antioxidant activity of Anaphalis lawii (Hook. f) Gamble and Helichrysum buddleioides DC - a comparative study
Abstract: The present study was conducted to determine antioxidant activity of two Asteraceae plants namely Anaphalis lawii (Hook. f) Gamble and Helichrysum buddleioides DC. The entire plants were shade dried and extracted by maceration process using methanol. Assays namely DPPH radical scavenging, ABTS radical scavenging, Nitric oxide scavenging, Ferric reducing and Iron chelating activity were performed to determine in vitro antioxidant activity of extract. In all assays, extract of H. buddleioides displayed marked activity when compared to extract of A. lawii. Folin-Ciocalteau reagent method and Aluminium chloride colorimetric estimation method were used to estimate the content of total phenolics and flavonoids respectively. Extract of H. buddleioides contained high phenolics and flavonoids when compared to A. lawii which might have accounted for marked antioxidant activity. The plants can be used against oxidative stress.
Key Words: Anaphalis lawii, Helichrysum buddleioides, Total phenolics and Total flavonoids
Abstract: The present study was conducted to determine antioxidant activity of two Asteraceae plants namely Anaphalis lawii (Hook. f) Gamble and Helichrysum buddleioides DC. The entire plants were shade dried and extracted by maceration process using methanol. Assays namely DPPH radical scavenging, ABTS radical scavenging, Nitric oxide scavenging, Ferric reducing and Iron chelating activity were performed to determine in vitro antioxidant activity of extract. In all assays, extract of H. buddleioides displayed marked activity when compared to extract of A. lawii. Folin-Ciocalteau reagent method and Aluminium chloride colorimetric estimation method were used to estimate the content of total phenolics and flavonoids respectively. Extract of H. buddleioides contained high phenolics and flavonoids when compared to A. lawii which might have accounted for marked antioxidant activity. The plants can be used against oxidative stress.
Key Words: Anaphalis lawii, Helichrysum buddleioides, Total phenolics and Total flavonoids
HOW TO CITE THIS ARTICLE?
APA (American Psychological Association)
Sandeepa, K. H., Harsha, T. S., Prashanth, M., Prashith Kekuda, T. R. and Raghavendra, H. L. (2017). In vitro antioxidant activity of Anaphalis lawii (Hook. f) Gamble and Helichrysum buddleioides DC- a comparative study. Journal of Bioscience and Agriculture Research, 12(02), 1064-1073.
MLA (Modern Language Association)
Sandeepa, K. H., Harsha, T. S., Prashanth, M., Prashith Kekuda, T. R. and Raghavendra, H. L. “In vitro antioxidant activity of Anaphalis lawii (Hook. f) Gamble and Helichrysum buddleioides DC- a comparative study”. Journal of Bioscience and Agriculture Research, 12.02(2017), 1064-1073.
Chicago and orTurabian
Sandeepa, K. H., Harsha, T. S., Prashanth, M., Prashith Kekuda, T. R. and Raghavendra, H. L. “In vitro antioxidant activity of Anaphalis lawii (Hook. f) Gamble and Helichrysum buddleioides DC- a comparative study”. Journal of Bioscience and Agriculture Research, 12. no. 02(2017), 1064-1073.
APA (American Psychological Association)
Sandeepa, K. H., Harsha, T. S., Prashanth, M., Prashith Kekuda, T. R. and Raghavendra, H. L. (2017). In vitro antioxidant activity of Anaphalis lawii (Hook. f) Gamble and Helichrysum buddleioides DC- a comparative study. Journal of Bioscience and Agriculture Research, 12(02), 1064-1073.
MLA (Modern Language Association)
Sandeepa, K. H., Harsha, T. S., Prashanth, M., Prashith Kekuda, T. R. and Raghavendra, H. L. “In vitro antioxidant activity of Anaphalis lawii (Hook. f) Gamble and Helichrysum buddleioides DC- a comparative study”. Journal of Bioscience and Agriculture Research, 12.02(2017), 1064-1073.
Chicago and orTurabian
Sandeepa, K. H., Harsha, T. S., Prashanth, M., Prashith Kekuda, T. R. and Raghavendra, H. L. “In vitro antioxidant activity of Anaphalis lawii (Hook. f) Gamble and Helichrysum buddleioides DC- a comparative study”. Journal of Bioscience and Agriculture Research, 12. no. 02(2017), 1064-1073.
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