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​You are here: Home>JBAR Journal>JBAR-Volume-16>jbar-160117-163.html

Journal of Bioscience and Agriculture Research 

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RESEARCH ARTICLE: 
In vitro regeneration protocol for indica rice genotypes
 
Kaium Miah (1), Billal Hossen (1), M. Shahidul Haque (2), M. Zablul Tareq (3) and Shamsun Nahar Begum (4)
1Dept .of Agricultural Extension, Ministry of Agriculture, Dhaka-1215
2Dept. of Biotechnology, Bangladesh Agricultural University, Mymensingh
3Jute Agriculture Experimental Station, Bangladesh Jute Research Institute, Manikganj
4Biotechnology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh 

Article info.
​Received: 11.08.17, Revised: 14.12.17, Available online: 31 December 2017.​
J. Bios. Agric. Res. | Volume 16, Issue 01, pp. 1314-1323
​Crossref: https://doi.org/10.18801/jbar.160117.163
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163.17.16.1_in_vitro_regeneration_protocol_for_indica_rice_genotypes.pdf
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Title: In vitro regeneration protocol for indica rice genotypes
Abstract: Embryogenic calli from mature seeds of four indica rice genotypes were used to observe their regeneration potentiality and establish a suitable in vitro plantlet regeneration protocol. MS medium supplemented with different phytohormone combinations were used to observe the callus induction ability of the explant. The highest callus induction (73.19%), biggest size of callus (3.133mm) and higher callus weight (0.7167g) were observed in Binadhan-6 in MS medium supplemented with 1.0 mg L-1 2,4-D over all the genotypes and MS medium supplemented with 1.5 mg L-1 2,4-D was the best over all the treatments (66.83%). Among the phytohormone combinations, MS + 8 mg L-1 Kinetin + 0.5 mg L-1 NAA showed the highest shoot regeneration (50.67%) and shoot length (13.7cm). Among the genotypes, Binadhan-6 was highly responsive to shoot regeneration (55.83%). The best root formation from regenerants (87.889%), maximum number of roots per plant (20) and the highest length (4.467 cm) of roots were in MS media supplemented with 0.5 mg L-1 IAA in Binadhan-6. In pot and soil, Binadhan-6 showed the highest survival rate of the plantlet 91.30% and 85%, respectively. This callus induction and in vitro regeneration protocol will be widely applicable for the tissue culture of indica rice.
 Key Words: Rice, In vitro callus induction, Protocol development and plant regeneration
HOW TO CITE THIS ARTICLE? ​​
APA (American Psychological Association)
Miah, K., Hossen, B., Haque, M. S., Tareq, M. Z. and Begum, S. N. (2017). In vitro regeneration protocol for indica rice genotypes. Journal of Bioscience and Agriculture Research,16(01), 1314-1323.
​
MLA (Modern Language Association) 
Miah, K., Hossen, B., Haque, M. S., Tareq, M. Z. and Begum, S. N. “In vitro regeneration protocol for indica rice genotypes”. Journal of Bioscience and Agriculture Research, 16.01(2017), 1314-1323.
​
Chicago and orTurabian
Miah, K., Hossen, B., Haque, M. S., Tareq, M. Z. and Begum, S. N. “In vitro regeneration protocol for indica rice genotypes”. Journal of Bioscience and Agriculture Research, 16. no. 01(2017), 1314-1323.
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