Journal of Bioscience and Agriculture Research |
|
RESEARCH ARTICLE:
In vitro regeneration protocol for indica rice genotypes
Kaium Miah (1), Billal Hossen (1), M. Shahidul Haque (2), M. Zablul Tareq (3) and Shamsun Nahar Begum (4)
1Dept .of Agricultural Extension, Ministry of Agriculture, Dhaka-1215
2Dept. of Biotechnology, Bangladesh Agricultural University, Mymensingh
3Jute Agriculture Experimental Station, Bangladesh Jute Research Institute, Manikganj
4Biotechnology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh
Article info.
Received: 11.08.17, Revised: 14.12.17, Available online: 31 December 2017.
J. Bios. Agric. Res. | Volume 16, Issue 01, pp. 1314-1323
Crossref: https://doi.org/10.18801/jbar.160117.163
In vitro regeneration protocol for indica rice genotypes
Kaium Miah (1), Billal Hossen (1), M. Shahidul Haque (2), M. Zablul Tareq (3) and Shamsun Nahar Begum (4)
1Dept .of Agricultural Extension, Ministry of Agriculture, Dhaka-1215
2Dept. of Biotechnology, Bangladesh Agricultural University, Mymensingh
3Jute Agriculture Experimental Station, Bangladesh Jute Research Institute, Manikganj
4Biotechnology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh
Article info.
Received: 11.08.17, Revised: 14.12.17, Available online: 31 December 2017.
J. Bios. Agric. Res. | Volume 16, Issue 01, pp. 1314-1323
Crossref: https://doi.org/10.18801/jbar.160117.163
FULL TEXT PDF:
| 163.17.16.1_in_vitro_regeneration_protocol_for_indica_rice_genotypes.pdf |
Title: In vitro regeneration protocol for indica rice genotypes
Abstract: Embryogenic calli from mature seeds of four indica rice genotypes were used to observe their regeneration potentiality and establish a suitable in vitro plantlet regeneration protocol. MS medium supplemented with different phytohormone combinations were used to observe the callus induction ability of the explant. The highest callus induction (73.19%), biggest size of callus (3.133mm) and higher callus weight (0.7167g) were observed in Binadhan-6 in MS medium supplemented with 1.0 mg L-1 2,4-D over all the genotypes and MS medium supplemented with 1.5 mg L-1 2,4-D was the best over all the treatments (66.83%). Among the phytohormone combinations, MS + 8 mg L-1 Kinetin + 0.5 mg L-1 NAA showed the highest shoot regeneration (50.67%) and shoot length (13.7cm). Among the genotypes, Binadhan-6 was highly responsive to shoot regeneration (55.83%). The best root formation from regenerants (87.889%), maximum number of roots per plant (20) and the highest length (4.467 cm) of roots were in MS media supplemented with 0.5 mg L-1 IAA in Binadhan-6. In pot and soil, Binadhan-6 showed the highest survival rate of the plantlet 91.30% and 85%, respectively. This callus induction and in vitro regeneration protocol will be widely applicable for the tissue culture of indica rice.
Key Words: Rice, In vitro callus induction, Protocol development and plant regeneration
Abstract: Embryogenic calli from mature seeds of four indica rice genotypes were used to observe their regeneration potentiality and establish a suitable in vitro plantlet regeneration protocol. MS medium supplemented with different phytohormone combinations were used to observe the callus induction ability of the explant. The highest callus induction (73.19%), biggest size of callus (3.133mm) and higher callus weight (0.7167g) were observed in Binadhan-6 in MS medium supplemented with 1.0 mg L-1 2,4-D over all the genotypes and MS medium supplemented with 1.5 mg L-1 2,4-D was the best over all the treatments (66.83%). Among the phytohormone combinations, MS + 8 mg L-1 Kinetin + 0.5 mg L-1 NAA showed the highest shoot regeneration (50.67%) and shoot length (13.7cm). Among the genotypes, Binadhan-6 was highly responsive to shoot regeneration (55.83%). The best root formation from regenerants (87.889%), maximum number of roots per plant (20) and the highest length (4.467 cm) of roots were in MS media supplemented with 0.5 mg L-1 IAA in Binadhan-6. In pot and soil, Binadhan-6 showed the highest survival rate of the plantlet 91.30% and 85%, respectively. This callus induction and in vitro regeneration protocol will be widely applicable for the tissue culture of indica rice.
Key Words: Rice, In vitro callus induction, Protocol development and plant regeneration
HOW TO CITE THIS ARTICLE?
APA (American Psychological Association)
Miah, K., Hossen, B., Haque, M. S., Tareq, M. Z. and Begum, S. N. (2017). In vitro regeneration protocol for indica rice genotypes. Journal of Bioscience and Agriculture Research,16(01), 1314-1323.
MLA (Modern Language Association)
Miah, K., Hossen, B., Haque, M. S., Tareq, M. Z. and Begum, S. N. “In vitro regeneration protocol for indica rice genotypes”. Journal of Bioscience and Agriculture Research, 16.01(2017), 1314-1323.
Chicago and orTurabian
Miah, K., Hossen, B., Haque, M. S., Tareq, M. Z. and Begum, S. N. “In vitro regeneration protocol for indica rice genotypes”. Journal of Bioscience and Agriculture Research, 16. no. 01(2017), 1314-1323.
APA (American Psychological Association)
Miah, K., Hossen, B., Haque, M. S., Tareq, M. Z. and Begum, S. N. (2017). In vitro regeneration protocol for indica rice genotypes. Journal of Bioscience and Agriculture Research,16(01), 1314-1323.
MLA (Modern Language Association)
Miah, K., Hossen, B., Haque, M. S., Tareq, M. Z. and Begum, S. N. “In vitro regeneration protocol for indica rice genotypes”. Journal of Bioscience and Agriculture Research, 16.01(2017), 1314-1323.
Chicago and orTurabian
Miah, K., Hossen, B., Haque, M. S., Tareq, M. Z. and Begum, S. N. “In vitro regeneration protocol for indica rice genotypes”. Journal of Bioscience and Agriculture Research, 16. no. 01(2017), 1314-1323.
- Azira, D. and Bhalla, P. L. (2000). Plant regeneration from mature embryo-derived callus of Australian rice (Oryza saliva L.) genotypes. Australian Journal of Agricultural Research 51, 305-312. https://doi.org/10.1071/AR98189
- BBS (2013). Bangladesh Bureau of Statistics. Crop Statistics (Major Crops). Agriculture Wing. Ministry of Planning. Government of the People’s Republic of Bangladesh, Dhaka. p. 54.
- Bhuiyan, S. I. and Karim, A. N. M. R. (2002). Rice production in Bangladesh: an overview. Bangladesh Rice Research Institute, Joydebpur, Dhaka. pp. 13-17.
- BRRI (2013). Homepage, Bangladesh Rice Research Institute (BRRI). www.BRRI.org.bd
- Chand, S. and Sahrawat, A. K. (2001). Stimulatory effect of partial desiccation on plant regeneration in indica rice (Oryza sativa L.). Journal of Plant Biochemistry and Biotechnology, 10, 43-47. https://doi.org/10.1007/BF03263105
- Dode, L. B., Goncalves, F. S. M., Oliveira, L. A. A., Vigh, L. L., Magalhees, J. A. M. and Peters, J. P. (2000). Effect of culture media hormonal balance in the induction and proliferation of mature rice seed derived callus. Revista-Cientifica-Rural, 5(2), 27-31.
- FAOSTAT (2012). Food and Agriculture Organization. www.faostat.fao.org
- Hidayat, U., Iltaf, U., Sultan, A. J. and Hamid, R. (2007). Tissue culture techniques for callus induction in rice. Sarhad Journal of Agriculture, 23(1), 82-86.
- Hoque, M. E. and Mansfield, I. W. (2004). Effect of genotype and explants age on callus induction and subsequent plant regeneration from root derived callus rice genotypes. Plant Cell, Tissue and Organ Culture, 78(3), 217-223.
- Ingram, H. M., Livesey, N. L., Power, J. B. and Davey, M. R. (2001). Genetic transformation of wheat: progress during the 1990s into the millennium, Acta Physiologiae Plantarum, 23(2), 221-239. https://doi.org/10.1007/s11738-001-0012-2
- Lee, K. S., Leon, H. S. and Kim, M. Y. (2002). Optimization of a mature embryo- based In Vitro culture system for high frequency somatic embryogenic callus induction and plant regeneration from japonica rice cultivars. Plant Cell, Tissue and Organ Culture, 71, 9-13.
- Lin, Y. J. and Zhang, Q. (2005). Optimizing the tissue culture condition for high efficiency transformation of indica rice. Plant Cell Reports, 23, 540-547. https://doi.org/10.1007/s00299-004-0843-6 PMid:15309499
- Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
- Saharan, V., Yadav, R. C., Yadav, N. R. and Chapagain, B. P. (2004) High frequency plant regeneration from desiccated calli of indica rice (Oryza sativa L.). African Journal of Biotechnology, 3(5), 256-259. https://doi.org/10.5897/AJB2004.000-2047
- Suresh, C., Sahrawat, A. K. and Chand, S. (2001). Stimulatory effect of partial desiccation on plant regeneration in indica rice (Oryza sativa L.). Journal of Plant Biochemistry and Biotechnology, 10(1), 43-47. https://doi.org/10.1007/BF03263105
- Vasil, I. K. (1998). Biotechnology and food security for the 21st century: a real-world perspective. Nature Biotechnology, 16, 399-400. https://doi.org/10.1038/nbt0598-399 PMid:9592375
- Visarada, K. B. R. S., Sailaja, M. and Sharma, N. P. (2002). Effect of callus induction media on morphology of embryogenic ealli in rice genotypes. Biologia Plantarum, 45(4), 495-502. https://doi.org/10.1023/A:1022323221513
- Visarada, K. B. R. S., Vrjaya Kumar, C. H. M. and Sharma, N. P. (2001). Analysis of variation in tissue culture response for identification of indica rice genotypes suitable for transformation. Journal of Genetics and Breeding, 55, 125-134.
- YuMei, D., FuYou, Y., LingXian, W., ZaiQuan, C. and XingQi, H. (2006). In vitro anther culture of three different genome types of Yunnan wild rice species. Southwest-China-Journal of Agricultural Science, 19(6), 1023-1027.
© 2017 The Authors. This article published by Journal BiNET is freely available for anyone to read, share, download, print, permitted for unrestricted use and build upon, provided that the original author(s) and publisher are given due credit. All Published articles are distributed under the Creative Commons Attribution 4.0 International License.
Require any edit or correction or changes in article? Please contact HERE.