Journal of Bioscience and Agriculture Research |
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RESEARCH ARTICLE:
Cultural and morphological characterization of Ceratocystis fambriata causing mango sudden death disease in district Muzaffargarh, Punjab, Pakistan and its chemical control
Shanam Naz, Abdul Rehman, Muhammad Zeshan Ahmed, Luqman Amrao and Salman Ghuffar
Dept. of plant pathology, University of Agriculture Faisalabad, Pakistan
Article info.
Received: 17.11.17, Revised: 15.02.18, Available online: 25 February 2018.
J. Bios. Agric. Res. | Volume 16, Issue 01, pp. 1330-1336
Crossref: https://doi.org/10.18801/jbar.160118.165
Cultural and morphological characterization of Ceratocystis fambriata causing mango sudden death disease in district Muzaffargarh, Punjab, Pakistan and its chemical control
Shanam Naz, Abdul Rehman, Muhammad Zeshan Ahmed, Luqman Amrao and Salman Ghuffar
Dept. of plant pathology, University of Agriculture Faisalabad, Pakistan
Article info.
Received: 17.11.17, Revised: 15.02.18, Available online: 25 February 2018.
J. Bios. Agric. Res. | Volume 16, Issue 01, pp. 1330-1336
Crossref: https://doi.org/10.18801/jbar.160118.165
FULL TEXT PDF:
| 165.18.16.1_cultural_and_morphological_characterization_of_ceratocystis_fambriata_causing_mango_sudden_death_disease.pdf |
Title: Cultural and morphological characterization of Ceratocystis fambriata causing mango sudden death disease in district Muzaffargarh, Punjab, Pakistan and its chemical control
Abstract: Mango (Mangifera indica L.) is an important fruit of Pakistan which is widely cultivated throughout the world. Fungal diseases of mango are the most important biological constraint to productivity. Out of which sudden death disease causes significant losses. The present investigation is carried out to characterize Ceratocystis fambriata causing mango sudden death disease and its management by application of different fungicides. During the survey 14 isolates of Ceratocystis fambriata causing sudden death disease was collected from Muzaffargarh district. The isolates were identified on the basis of cultural and microscopic features. After 6 days during cultural studies maximum growth was observed on Czepk and Malt extract medium (85 mm) and minimum growth was on PDA (74.1 mm). The colonies colors range from grayish, slightly white to light brown having smooth, circular and regular or irregular margins on the surface. Perithecia was black to brown, smooth, circular and regular or irregular, Aascospore was hat shaped ranging 4.18- 6.34 μmx 3.15 - 4.88 μm, Aleurioconidia ranging 17.1- 18.5 μmx 9.04-10.09 μm, Endoconidia ranging 22.5-24.2 μmx 3.90-5.30 μm. During pathogenicity test, result showed that infection started after fifteen days of inoculation. The pathogen covered 1.3 cm after fifteen days. Final reading was taken on the forty five day, when the pathogen colonized up to 3.25 cm. Among different fungicides the results after 2, 4 and 6 days revealed that Topsin- M was found most effective for controlling the growth of Pathogen that showed colony diameter 0.20, 0.50 and 1.3 cm at 200 ppm followed by Score, Nativo, Cabriotop and Copper oxychloride respectively.
Key Words: Mango, Ceratocystis fambriata, Chemical control and Muzaffargarh
Abstract: Mango (Mangifera indica L.) is an important fruit of Pakistan which is widely cultivated throughout the world. Fungal diseases of mango are the most important biological constraint to productivity. Out of which sudden death disease causes significant losses. The present investigation is carried out to characterize Ceratocystis fambriata causing mango sudden death disease and its management by application of different fungicides. During the survey 14 isolates of Ceratocystis fambriata causing sudden death disease was collected from Muzaffargarh district. The isolates were identified on the basis of cultural and microscopic features. After 6 days during cultural studies maximum growth was observed on Czepk and Malt extract medium (85 mm) and minimum growth was on PDA (74.1 mm). The colonies colors range from grayish, slightly white to light brown having smooth, circular and regular or irregular margins on the surface. Perithecia was black to brown, smooth, circular and regular or irregular, Aascospore was hat shaped ranging 4.18- 6.34 μmx 3.15 - 4.88 μm, Aleurioconidia ranging 17.1- 18.5 μmx 9.04-10.09 μm, Endoconidia ranging 22.5-24.2 μmx 3.90-5.30 μm. During pathogenicity test, result showed that infection started after fifteen days of inoculation. The pathogen covered 1.3 cm after fifteen days. Final reading was taken on the forty five day, when the pathogen colonized up to 3.25 cm. Among different fungicides the results after 2, 4 and 6 days revealed that Topsin- M was found most effective for controlling the growth of Pathogen that showed colony diameter 0.20, 0.50 and 1.3 cm at 200 ppm followed by Score, Nativo, Cabriotop and Copper oxychloride respectively.
Key Words: Mango, Ceratocystis fambriata, Chemical control and Muzaffargarh
HOW TO CITE THIS ARTICLE?
APA (American Psychological Association)
Naz, S., Rehman, A., Ahmed, M. Z., Amrao, L. and Ghuffar, S. (2018). Cultural and morphological characterization of Ceratocystis fambriata causing mango sudden death disease in district Muzaffargarh, Punjab, Pakistan and its chemical control. Journal of Bioscience and Agriculture Research,16(01), 1330-1336.
MLA (Modern Language Association)
Naz, S., Rehman, A., Ahmed, M. Z., Amrao, L. and Ghuffar, S. “Cultural and morphological characterization of Ceratocystis fambriata causing mango sudden death disease in district Muzaffargarh, Punjab, Pakistan and its chemical control”. Journal of Bioscience and Agriculture Research, 16.01(2018), 1330-1336.
Chicago and orTurabian
Naz, S., Rehman, A., Ahmed, M. Z., Amrao, L. and Ghuffar, S. “Effect of seed priming on growth and physiological traits of five Jordanian wheat (Triticum aestivum L.) landraces under salt stress”. Journal of Bioscience and Agriculture Research, 16. no. 01(2018), 1330-1336.
APA (American Psychological Association)
Naz, S., Rehman, A., Ahmed, M. Z., Amrao, L. and Ghuffar, S. (2018). Cultural and morphological characterization of Ceratocystis fambriata causing mango sudden death disease in district Muzaffargarh, Punjab, Pakistan and its chemical control. Journal of Bioscience and Agriculture Research,16(01), 1330-1336.
MLA (Modern Language Association)
Naz, S., Rehman, A., Ahmed, M. Z., Amrao, L. and Ghuffar, S. “Cultural and morphological characterization of Ceratocystis fambriata causing mango sudden death disease in district Muzaffargarh, Punjab, Pakistan and its chemical control”. Journal of Bioscience and Agriculture Research, 16.01(2018), 1330-1336.
Chicago and orTurabian
Naz, S., Rehman, A., Ahmed, M. Z., Amrao, L. and Ghuffar, S. “Effect of seed priming on growth and physiological traits of five Jordanian wheat (Triticum aestivum L.) landraces under salt stress”. Journal of Bioscience and Agriculture Research, 16. no. 01(2018), 1330-1336.
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