RESEARCH ARTICLE:
Shoot multiplication of gerbera callus grown from flower buds under different growth regulators composition
S. Parvin, N. Sabrin, M. R. Jahan, H. Ahmad, and A. F. M. Jamal Uddin
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh
ARTICLE INFORMATION:
Received date: 14.01.18, Revised date: 04.09.18, First available online: 14 October 2018.
J. Bios. Agric. Res. | Volume 17, Issue 02, Page number 1442-1447
Crossref DOI: https://doi.org/10.18801/jbar.170218.179
Shoot multiplication of gerbera callus grown from flower buds under different growth regulators composition
S. Parvin, N. Sabrin, M. R. Jahan, H. Ahmad, and A. F. M. Jamal Uddin
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh
ARTICLE INFORMATION:
Received date: 14.01.18, Revised date: 04.09.18, First available online: 14 October 2018.
J. Bios. Agric. Res. | Volume 17, Issue 02, Page number 1442-1447
Crossref DOI: https://doi.org/10.18801/jbar.170218.179
FULL TEXT PDF:
179.17.02.18_shoot_multiplication_of_gerbera_callus_grown_from_flower_buds_under_different_growth_regulators_composition.pdf | |
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Shoot multiplication of gerbera callus grown from flower buds under different growth regulators composition
Abstract
An experiment was conducted in at Advanced Seed Research and Biotech Center (ASRBC), ACI Limited; Dhaka, Bangladesh during the period of September to October, 2014 to evaluate different growth regulator composition towards shooting from callus produced using the flower bud of selected mother plant of gerbera. Callus grown from flower buds were subjected to six growth regulator composition viz.T0: 0 mg/l BAP + 0 mg /l NAA, T1: 1 mg/l BAP + 0.01 mg /l NAA, T2:2 mg/l BAP + 0.01 mg /l NAA, T3: 3 mg/l BAP + 0.01 mg /l NAA, T4:4 mg/l BAP + 0.01 mg /l NAA, T5:5 mg/l BAP + 0.01 mg /l NAA. Number of responsive explants (34.3), Percent (%) of responsive explants (85.8%), Number of days taken for shoot initiation (16 days), Number of shoots per clump (8.0), Number of leaves per shoot (7.70), Shoot length (25.4 mm) were found best in T3 whereas the lowest was observed from T1 and T5. Thus, combination of 3 mg/l BAP + 0.01 mg /l NAA can be considered as the best composition of growth regulators for shoot multiplication of gerbera callus grown from flower buds.
Key Words: Shoot multiplication, BAP, NAA and Protocol development
Abstract
An experiment was conducted in at Advanced Seed Research and Biotech Center (ASRBC), ACI Limited; Dhaka, Bangladesh during the period of September to October, 2014 to evaluate different growth regulator composition towards shooting from callus produced using the flower bud of selected mother plant of gerbera. Callus grown from flower buds were subjected to six growth regulator composition viz.T0: 0 mg/l BAP + 0 mg /l NAA, T1: 1 mg/l BAP + 0.01 mg /l NAA, T2:2 mg/l BAP + 0.01 mg /l NAA, T3: 3 mg/l BAP + 0.01 mg /l NAA, T4:4 mg/l BAP + 0.01 mg /l NAA, T5:5 mg/l BAP + 0.01 mg /l NAA. Number of responsive explants (34.3), Percent (%) of responsive explants (85.8%), Number of days taken for shoot initiation (16 days), Number of shoots per clump (8.0), Number of leaves per shoot (7.70), Shoot length (25.4 mm) were found best in T3 whereas the lowest was observed from T1 and T5. Thus, combination of 3 mg/l BAP + 0.01 mg /l NAA can be considered as the best composition of growth regulators for shoot multiplication of gerbera callus grown from flower buds.
Key Words: Shoot multiplication, BAP, NAA and Protocol development
HOW TO CITE THIS ARTICLE?
MLA
Parvin et al. “Shoot multiplication of gerbera callus grown from flower buds under different growth regulators composition.” Journal of Bioscience and Agriculture Research 17(02) (2018): 1442-1447.
APA
Parvin, S., Sabrin, N., Jahan, M. R., Ahmad, H. and Jamal Uddin, A. F. M. (2018). Shoot multiplication of gerbera callus grown from flower buds under different growth regulators composition. Journal of Bioscience and Agriculture Research, 17(02), 1442-1447.
Chicago
Parvin, S., Sabrin, N., Jahan, M. R., Ahmad, H. and Jamal Uddin, A. F. M. “Shoot multiplication of gerbera callus grown from flower buds under different growth regulators composition.” Journal of Bioscience and Agriculture Research 17(02) (2018): 1442-1447.
Harvard
Parvin, S., Sabrin, N., Jahan, M. R., Ahmad, H. and Jamal Uddin, A. F. M. 2018. Shoot multiplication of gerbera callus grown from flower buds under different growth regulators composition. Journal of Bioscience and Agriculture Research, 17(02), pp. 1442-1447.
Vancouver
Parvin, S, Sabrin, N, Jahan, MR, Ahmad, H and Jamal Uddin, AFM. Shoot multiplication of gerbera callus grown from flower buds under different growth regulators composition. Journal of Bioscience and Agriculture Research. 2018 October 17(02): 1442-1447.
MLA
Parvin et al. “Shoot multiplication of gerbera callus grown from flower buds under different growth regulators composition.” Journal of Bioscience and Agriculture Research 17(02) (2018): 1442-1447.
APA
Parvin, S., Sabrin, N., Jahan, M. R., Ahmad, H. and Jamal Uddin, A. F. M. (2018). Shoot multiplication of gerbera callus grown from flower buds under different growth regulators composition. Journal of Bioscience and Agriculture Research, 17(02), 1442-1447.
Chicago
Parvin, S., Sabrin, N., Jahan, M. R., Ahmad, H. and Jamal Uddin, A. F. M. “Shoot multiplication of gerbera callus grown from flower buds under different growth regulators composition.” Journal of Bioscience and Agriculture Research 17(02) (2018): 1442-1447.
Harvard
Parvin, S., Sabrin, N., Jahan, M. R., Ahmad, H. and Jamal Uddin, A. F. M. 2018. Shoot multiplication of gerbera callus grown from flower buds under different growth regulators composition. Journal of Bioscience and Agriculture Research, 17(02), pp. 1442-1447.
Vancouver
Parvin, S, Sabrin, N, Jahan, MR, Ahmad, H and Jamal Uddin, AFM. Shoot multiplication of gerbera callus grown from flower buds under different growth regulators composition. Journal of Bioscience and Agriculture Research. 2018 October 17(02): 1442-1447.
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