J. Bios. Agric. Res. | Volume 18, Issue 01, 1496-1511| https://doi.org/10.18801/jbar.180118.185
Article type: Research article, Received: 05.08.2018, Revised: 03.12.2018, Date of Publication: 14 December 2018.
Article type: Research article, Received: 05.08.2018, Revised: 03.12.2018, Date of Publication: 14 December 2018.
Morphological based screening and genetic diversity analysis of the local rice (Oryza sativa L.) landraces at the seedling stage for salinity tolerance
Iffat Eti, Md. Rasel, Lutful Hassan and Aleya Ferdausi
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensing-2202, Bangladesh
Abstract
Salinity is considered as one of major threats in rice production around the world. This experiment was conducted to screen out the salt tolerant rice genotypes following modified hydroponic method and IRRI standard protocol (SES scoring). Twenty-five rice genotypes were evaluated for the screening purpose for salinity tolerance considering morphological parameters. Three levels of salinity treatments (EC-0 dSm-1, EC-7 dSm-1 and EC- 12 dSm-1) were used for the phenotypic analysis and genotypes were categorized according to SES scoring based on visual salt injury in rice seedlings at 21th days of saline treatment. Salt injury symptoms varied among the landraces with different concentration of salt. All plant parameters reduced significantly in all genotypes with increasing salinity although less reduction was found in some genotypes at higher salinity also and identified those genotypes as salt tolerant. After 21 days of salinization, five genotypes (Maloti, Chinisagor, Lal bat, Moyna, Binadhan-8 and Binadhan-10) were found as slat tolerant at both 7 dSm-1 and 12 dSm-1 according to standard evaluation score based on visual salt injury at seedling stage. The phenotypic co-efficient of variation (PCV) was higher than genotypic co-efficient of variation (GCV) for all the traits studied indicating that they all are interacted with the environment to some extent. The highest heritability was found for all traits in the range of 73.82% to 96.08% indicating that the traits are less influenced by environment and these traits can be considered for the improvement of salinity tolerance. High heritability coupled with high genetic advance as percent of mean was observed for standard evaluation score (96.08%, 130.51%), root dry weight (95.72%, 135.08%) and shoot fresh weight (94.63%, 68.38%) indicating the role of additive gene expression for these traits and would facilitate better scope for improvement of these traits through direct selection. The correlation and path analysis showed that live leaves (%), survival rate (%), shoot length, chlorophyll content, root fresh weight, root dry weight had significant negative correlation with standard evaluation score as well as had direct positive effect on standard evaluation score indicating their importance for the improvement of salt tolerance ability of plant.
Key Words: Rice, Salinity, Heritability, Genetic advance and Path analysis
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensing-2202, Bangladesh
Abstract
Salinity is considered as one of major threats in rice production around the world. This experiment was conducted to screen out the salt tolerant rice genotypes following modified hydroponic method and IRRI standard protocol (SES scoring). Twenty-five rice genotypes were evaluated for the screening purpose for salinity tolerance considering morphological parameters. Three levels of salinity treatments (EC-0 dSm-1, EC-7 dSm-1 and EC- 12 dSm-1) were used for the phenotypic analysis and genotypes were categorized according to SES scoring based on visual salt injury in rice seedlings at 21th days of saline treatment. Salt injury symptoms varied among the landraces with different concentration of salt. All plant parameters reduced significantly in all genotypes with increasing salinity although less reduction was found in some genotypes at higher salinity also and identified those genotypes as salt tolerant. After 21 days of salinization, five genotypes (Maloti, Chinisagor, Lal bat, Moyna, Binadhan-8 and Binadhan-10) were found as slat tolerant at both 7 dSm-1 and 12 dSm-1 according to standard evaluation score based on visual salt injury at seedling stage. The phenotypic co-efficient of variation (PCV) was higher than genotypic co-efficient of variation (GCV) for all the traits studied indicating that they all are interacted with the environment to some extent. The highest heritability was found for all traits in the range of 73.82% to 96.08% indicating that the traits are less influenced by environment and these traits can be considered for the improvement of salinity tolerance. High heritability coupled with high genetic advance as percent of mean was observed for standard evaluation score (96.08%, 130.51%), root dry weight (95.72%, 135.08%) and shoot fresh weight (94.63%, 68.38%) indicating the role of additive gene expression for these traits and would facilitate better scope for improvement of these traits through direct selection. The correlation and path analysis showed that live leaves (%), survival rate (%), shoot length, chlorophyll content, root fresh weight, root dry weight had significant negative correlation with standard evaluation score as well as had direct positive effect on standard evaluation score indicating their importance for the improvement of salt tolerance ability of plant.
Key Words: Rice, Salinity, Heritability, Genetic advance and Path analysis
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MLA
Eti, et al. "Morphological Based Screening and Genetic Diversity Analysis of the Local Rice (Oryza Sativa L.) Landraces at the Seedling Stage for Salinity Tolerance." Journal of Bioscience and Agriculture Research 18 (1) (2018): 1496-11.
APA
Eti, I., Rasel, M., Hassan, L., & Ferdausi, A. (2018). Morphological based screening and genetic diversity analysis of the local rice (Oryza sativa L.) landraces at the seedling stage for salinity tolerance. Journal of Bioscience and Agriculture Research, 18(1), 1496-1511.
Chicago
Eti Iffat, Md. Rasel, Lutful Hassan and Aleya Ferdausi. "Morphological based screening and genetic diversity analysis of the local rice (Oryza sativa L.) landraces at the seedling stage for salinity tolerance." Journal of Bioscience and Agriculture Research 18 (1) (2018): 1496-1511.
Harvard
Eti, I., Rasel, M., Hassan, L. and Ferdausi, A. 2018. Morphological based screening and genetic diversity analysis of the local rice (Oryza sativa L.) landraces at the seedling stage for salinity tolerance. Journal of Bioscience and Agriculture Research, 18 (01), pp. 1496-1511.
Vancouver
Eti I, Rasel M, Hassan L, Ferdausi A. Morphological based screening and genetic diversity analysis of the local rice (Oryza sativa L.) landraces at the seedling stage for salinity tolerance. Journal of Bioscience and Agriculture Research. 2018 December 18(1):1496-1511.
Eti, et al. "Morphological Based Screening and Genetic Diversity Analysis of the Local Rice (Oryza Sativa L.) Landraces at the Seedling Stage for Salinity Tolerance." Journal of Bioscience and Agriculture Research 18 (1) (2018): 1496-11.
APA
Eti, I., Rasel, M., Hassan, L., & Ferdausi, A. (2018). Morphological based screening and genetic diversity analysis of the local rice (Oryza sativa L.) landraces at the seedling stage for salinity tolerance. Journal of Bioscience and Agriculture Research, 18(1), 1496-1511.
Chicago
Eti Iffat, Md. Rasel, Lutful Hassan and Aleya Ferdausi. "Morphological based screening and genetic diversity analysis of the local rice (Oryza sativa L.) landraces at the seedling stage for salinity tolerance." Journal of Bioscience and Agriculture Research 18 (1) (2018): 1496-1511.
Harvard
Eti, I., Rasel, M., Hassan, L. and Ferdausi, A. 2018. Morphological based screening and genetic diversity analysis of the local rice (Oryza sativa L.) landraces at the seedling stage for salinity tolerance. Journal of Bioscience and Agriculture Research, 18 (01), pp. 1496-1511.
Vancouver
Eti I, Rasel M, Hassan L, Ferdausi A. Morphological based screening and genetic diversity analysis of the local rice (Oryza sativa L.) landraces at the seedling stage for salinity tolerance. Journal of Bioscience and Agriculture Research. 2018 December 18(1):1496-1511.
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