J. Biosci. Agric. Res. | Volume 23, Issue 02, 1937-1943 | https://doi.org/10.18801/jbar.230220.238
Article type: Research article | Received: 14.01.2020; Revised: 20.03.2020; First published online: 14 April 2020.
Article type: Research article | Received: 14.01.2020; Revised: 20.03.2020; First published online: 14 April 2020.
Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in Sasso and Shika brown chickens
Agaviezor, B. O. Ajayi, F. O. and Udoudo, O. A.
Animal Science Department, University of Port Harcourt, Port Harcourt, Nigeria.
✉ For any information: ask.author@journalbinet.com.
Animal Science Department, University of Port Harcourt, Port Harcourt, Nigeria.
✉ For any information: ask.author@journalbinet.com.
Abstract
This study was conducted to check the association between PIT 1 gene and some carcass traits in 30 Sasso and 30 Shika brown chickens. Growth data were collected for 12 weeks. DNA was extracted from the blood of the chickens using the Zymo spin column text kit by Zymo Research. Polymerase Chain Reaction (PCR) was done using PIT 1 primers and PCR products were digested with MSP1 restriction enzyme to generate the genotypes. Data generated were analyzed using SPSS Version 16, MEGA 6.0 and DNASP. The results showed allele frequency of A in Sasso was 0.4375 while in Sasso, it was 0.5714. Allele frequency of B was 0.5625 in Sasso 0.4286 in Shika brown. The heterozygous frequency was 0.3750 in Sasso and 0.2857 in Shika brown. The highest expected frequency was in AB genotype both in Sasso (3.9375) and in Shika brown (3.4286). Hardy-Weinberg equilibrium was not significant (p>0.05) in Sasso chickens. In week 1, the highest body weight occurred in the genotype (BB) 83.42g. However, the highest value for body length was observed in the genotype (AA) 9.00cm in week 5, the highest body weight occurred in the genotype (AB) in Sasso 545.00±121.74g. The results exposed variation in the expression in PIT1 gene in carcass traits which could be exploited in chicken breeding programmes.
Key Words: PIT 1, Association, Sasso, Shika brown and Carcass
This study was conducted to check the association between PIT 1 gene and some carcass traits in 30 Sasso and 30 Shika brown chickens. Growth data were collected for 12 weeks. DNA was extracted from the blood of the chickens using the Zymo spin column text kit by Zymo Research. Polymerase Chain Reaction (PCR) was done using PIT 1 primers and PCR products were digested with MSP1 restriction enzyme to generate the genotypes. Data generated were analyzed using SPSS Version 16, MEGA 6.0 and DNASP. The results showed allele frequency of A in Sasso was 0.4375 while in Sasso, it was 0.5714. Allele frequency of B was 0.5625 in Sasso 0.4286 in Shika brown. The heterozygous frequency was 0.3750 in Sasso and 0.2857 in Shika brown. The highest expected frequency was in AB genotype both in Sasso (3.9375) and in Shika brown (3.4286). Hardy-Weinberg equilibrium was not significant (p>0.05) in Sasso chickens. In week 1, the highest body weight occurred in the genotype (BB) 83.42g. However, the highest value for body length was observed in the genotype (AA) 9.00cm in week 5, the highest body weight occurred in the genotype (AB) in Sasso 545.00±121.74g. The results exposed variation in the expression in PIT1 gene in carcass traits which could be exploited in chicken breeding programmes.
Key Words: PIT 1, Association, Sasso, Shika brown and Carcass
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Article Citations
MLA
Agaviezor et al. “Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens.” Journal of Bioscience and Agriculture Research 23(02) (2020): 1937-1943.
APA
Agaviezor, B. O. Ajayi, F. O. and Udoudo, O. A. (2020). Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens. Journal of Bioscience and Agriculture Research, 23(02), 1937-1943.
Chicago
Agaviezor, B. O. Ajayi, F. O. and Udoudo, O. A. “Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens.” Journal of Bioscience and Agriculture Research 23(02) (2020): 1937-1943.
Harvard
Agaviezor, B. O. Ajayi, F. O. and Udoudo, O. A. 2020. Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens. Journal of Bioscience and Agriculture Research, 23(02), pp. 1937-1943.
Vancouver
Agaviezor BO, Ajayi FO and Udoudo OA. Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens. 2020 April 23(02):1937-1943.
Agaviezor et al. “Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens.” Journal of Bioscience and Agriculture Research 23(02) (2020): 1937-1943.
APA
Agaviezor, B. O. Ajayi, F. O. and Udoudo, O. A. (2020). Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens. Journal of Bioscience and Agriculture Research, 23(02), 1937-1943.
Chicago
Agaviezor, B. O. Ajayi, F. O. and Udoudo, O. A. “Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens.” Journal of Bioscience and Agriculture Research 23(02) (2020): 1937-1943.
Harvard
Agaviezor, B. O. Ajayi, F. O. and Udoudo, O. A. 2020. Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens. Journal of Bioscience and Agriculture Research, 23(02), pp. 1937-1943.
Vancouver
Agaviezor BO, Ajayi FO and Udoudo OA. Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens. 2020 April 23(02):1937-1943.
References
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