J. Biosci. Agric. Res. | Volume 23, Issue 02, 1937-1943 | https://doi.org/10.18801/jbar.230220.238
Article type: Research article | Received: 14.01.2020; Revised: 20.03.2020; First published online: 14 April 2020.
Article type: Research article | Received: 14.01.2020; Revised: 20.03.2020; First published online: 14 April 2020.
Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in Sasso and Shika brown chickens
Agaviezor, B. O. Ajayi, F. O. and Udoudo, O. A.
Animal Science Department, University of Port Harcourt, Port Harcourt, Nigeria.
✉ For any information: [email protected].
Animal Science Department, University of Port Harcourt, Port Harcourt, Nigeria.
✉ For any information: [email protected].
Abstract
This study was conducted to check the association between PIT 1 gene and some carcass traits in 30 Sasso and 30 Shika brown chickens. Growth data were collected for 12 weeks. DNA was extracted from the blood of the chickens using the Zymo spin column text kit by Zymo Research. Polymerase Chain Reaction (PCR) was done using PIT 1 primers and PCR products were digested with MSP1 restriction enzyme to generate the genotypes. Data generated were analyzed using SPSS Version 16, MEGA 6.0 and DNASP. The results showed allele frequency of A in Sasso was 0.4375 while in Sasso, it was 0.5714. Allele frequency of B was 0.5625 in Sasso 0.4286 in Shika brown. The heterozygous frequency was 0.3750 in Sasso and 0.2857 in Shika brown. The highest expected frequency was in AB genotype both in Sasso (3.9375) and in Shika brown (3.4286). Hardy-Weinberg equilibrium was not significant (p>0.05) in Sasso chickens. In week 1, the highest body weight occurred in the genotype (BB) 83.42g. However, the highest value for body length was observed in the genotype (AA) 9.00cm in week 5, the highest body weight occurred in the genotype (AB) in Sasso 545.00±121.74g. The results exposed variation in the expression in PIT1 gene in carcass traits which could be exploited in chicken breeding programmes.
Key Words: PIT 1, Association, Sasso, Shika brown and Carcass
This study was conducted to check the association between PIT 1 gene and some carcass traits in 30 Sasso and 30 Shika brown chickens. Growth data were collected for 12 weeks. DNA was extracted from the blood of the chickens using the Zymo spin column text kit by Zymo Research. Polymerase Chain Reaction (PCR) was done using PIT 1 primers and PCR products were digested with MSP1 restriction enzyme to generate the genotypes. Data generated were analyzed using SPSS Version 16, MEGA 6.0 and DNASP. The results showed allele frequency of A in Sasso was 0.4375 while in Sasso, it was 0.5714. Allele frequency of B was 0.5625 in Sasso 0.4286 in Shika brown. The heterozygous frequency was 0.3750 in Sasso and 0.2857 in Shika brown. The highest expected frequency was in AB genotype both in Sasso (3.9375) and in Shika brown (3.4286). Hardy-Weinberg equilibrium was not significant (p>0.05) in Sasso chickens. In week 1, the highest body weight occurred in the genotype (BB) 83.42g. However, the highest value for body length was observed in the genotype (AA) 9.00cm in week 5, the highest body weight occurred in the genotype (AB) in Sasso 545.00±121.74g. The results exposed variation in the expression in PIT1 gene in carcass traits which could be exploited in chicken breeding programmes.
Key Words: PIT 1, Association, Sasso, Shika brown and Carcass
Article Full-Text PDF
| 238.02.23.2020_carcass_traits_in_sasso_and_shika_brown_chickens_in_nigeria.pdf |
Article Metrics
|
Share This Article
|
|
Article Citations
MLA
Agaviezor et al. “Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens.” Journal of Bioscience and Agriculture Research 23(02) (2020): 1937-1943.
APA
Agaviezor, B. O. Ajayi, F. O. and Udoudo, O. A. (2020). Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens. Journal of Bioscience and Agriculture Research, 23(02), 1937-1943.
Chicago
Agaviezor, B. O. Ajayi, F. O. and Udoudo, O. A. “Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens.” Journal of Bioscience and Agriculture Research 23(02) (2020): 1937-1943.
Harvard
Agaviezor, B. O. Ajayi, F. O. and Udoudo, O. A. 2020. Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens. Journal of Bioscience and Agriculture Research, 23(02), pp. 1937-1943.
Vancouver
Agaviezor BO, Ajayi FO and Udoudo OA. Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens. 2020 April 23(02):1937-1943.
Agaviezor et al. “Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens.” Journal of Bioscience and Agriculture Research 23(02) (2020): 1937-1943.
APA
Agaviezor, B. O. Ajayi, F. O. and Udoudo, O. A. (2020). Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens. Journal of Bioscience and Agriculture Research, 23(02), 1937-1943.
Chicago
Agaviezor, B. O. Ajayi, F. O. and Udoudo, O. A. “Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens.” Journal of Bioscience and Agriculture Research 23(02) (2020): 1937-1943.
Harvard
Agaviezor, B. O. Ajayi, F. O. and Udoudo, O. A. 2020. Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens. Journal of Bioscience and Agriculture Research, 23(02), pp. 1937-1943.
Vancouver
Agaviezor BO, Ajayi FO and Udoudo OA. Association between pituitary specific transcription factor 1 gene (pit 1) and some carcass traits in sasso and shika brown chickens. 2020 April 23(02):1937-1943.
References
- Adebambo, A. O. Bjørnstad, G. Bulimo, W. Jianlin, H. Kierstein, G. Mazhani, L. Podisid, B. Hirbo, J. Agyemang, K. Wollny, C. Gondwe, T. Zeuh, V. Tadelle, D. Abebe, G. Abdoulaye, P. Pacoi, S. Serunjogi, L. Abrerrahman, M. Sow, R. Weigend, S. Sanfo, R. Gaye, F. Ssewanyana, E. Coulibalyk, M. D. Teme, B. VSF and Hanotte, O. (2009). Mitochondrial DNA D-Loop analysis of South Western Nigerian Chicken. Arch. Zootec. 58 (224), 637-643.
- Agaviezor, B. O. Ajayi, F. O. and Nwachukwu, C. C. (2018). Single nucleotide polymorphism in growth hormone gene and its association with growth performance in chicken. African Journal of Agriculture, Technology and Environment, 7(2), 188 – 194.
- Haruna, E. S. Adene, D. F. Gerrit, V. J. (2003). Detection of Infectious Bursal Disease Virus (IBDV) in naturally infected chickens in Nigeria by the Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Nigerian Veterinary Journal, 24(2), 1-9.
- Jiang, R. Li, J. Qu, L. Li, H. and Yang, N. (2004). A new single-nu-cleotide polymorphism in the chicken pituitary-specific transcrip-tion factor (POU1F1) gene associated with growth rate. Anim. Genet. 35, 344–346. https://doi.org/10.1111/j.1365-2052.2004.01164.x
- Librado, P. and Rozasm J. (2009). DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25: 1451 – 1452.
- Miller, M. P. (1997). Tools for population genetic analysis (TFPGA) 1.3: A windows programme for the analysis of allozyme and molecular population genetic data. Computer software distributed by author.
- Nie, Q. Fang, M. Xie, L. Zhou, M. Liang, Z. Luo, Z. … Zhang, X. (2008). The PIT1 gene polymorphisms were associated with chicken growth traits. BMC genetics, 9, 20.
- Niknafs, S. Javaremi, A. N. and Sadeghi, M. (2013). Single nucleotide polymorphism in BMPR-IB and STAT5B genes and their Association with growth and reproductive traits in chicken. Songklanakarin Journal of Science and Technology, 36(2), 137-142.
- Rodbari, Z. Alipanah, M. Seyedabadi, H. R. and Amirinia, C. (2011). Identification of a single nucleotide polymorphism of the pituitary-specific transcriptional factor 1 (Pit 1) gene and its association with body composition trait in Iranian commercial broiler line. African Journal of Biotechnology, 10(60), 12979-12983. https://doi.org/10.5897/AJB11.1149
- Tamura, K. Stecher, G. Peterson, D. Filipski, A. and Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725-2729.
- Tatsumi, K. Notomi, T. Amino, N. and Miyai, K. (1992). Nucleotide sequence of the complementary DNA for human Pit-1/GHF-1. Biochim Biophys Acta, 231–234.
- Yan, L. J. Fang, X. T. Liu, Y. Zhang, C. L. Zhoa, J. and Chen, H. (2013). Effects of single and combined Genotypes of MC4R and POU1F1 genes on two production traits in Langshan chicken. Journal of Molecular Biology, 40, 4645-4650. https://doi.org/10.1007/s11033-013-2558-7
- Zhoa, H. Li, M. Xu, S. S. and Liu, G. (2015). Single Nucleotide polymorphisms in IGFBP-2 Gene and their Association with body weight traits on Jinghai yellow chicken. Brazilian Journal of poultry science, 17(4), 497-502. https://doi.org/10.1590/1516-635X1704497-502
© 2020 The Authors. This article is freely available for anyone to read, share, download, print, permitted for unrestricted use and build upon, provided that the original author(s) and publisher are given due credit. All Published articles are distributed under the Creative Commons Attribution 4.0 International License.
Journal of Bioscience and Agriculture Research EISSN 2312-7945.