J. Biosci. Agric. Res. | Volume 24, Issue 01, 1990-1998 | https://doi.org/10.18801/jbar.240120.243
Article type: Research article | Received: 08.02.2020; Revised: 30.03.2020; First published online: 25 April 2020
Article type: Research article | Received: 08.02.2020; Revised: 30.03.2020; First published online: 25 April 2020
Effect of phytohormone on shoot generation potentiality in rucola
Sadia Afrin 1, Md. Abu Zafur Al Munsur 3, Md. Shahidul Islam Khan 2, Fahima Khatun 1 and M. E. Hoque 1
1 Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka-1207
2 Department of Entomology, Sher-e-Bangla Agricultural University, Dhaka-1207
3 Department of Agricultural Extension, Khamarbari, Dhaka-1207, Bangladesh.
✉ Corresponding author: almunsurdae@gmail.com (Munsur, MAZA)
1 Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka-1207
2 Department of Entomology, Sher-e-Bangla Agricultural University, Dhaka-1207
3 Department of Agricultural Extension, Khamarbari, Dhaka-1207, Bangladesh.
✉ Corresponding author: almunsurdae@gmail.com (Munsur, MAZA)
Abstract
The present investigation was carried out to study the in vitro regeneration in Rucola. Different concentrations and combinations of BA and IBA were used for regeneration of root and shoot in rucola. Cotyledonary nodes were used as explants. The highest number of shoots (4.60) and leaves (6.4) were recorded in treatment 4.0 mg/l BA with minimum (9.00) days. The maximum days (14.00) was noticed in a simple MS medium to regenerate lower no. of shoots. The treatment BA 4.0 mg/l produced the highest number of the main root (4.60) while it was lowest in the control treatment. The highest number of shoot (3.40) was noticed from the BA 4.0 mg/l + IBA 3.0 mg/l. The treatment BA 4.0 mg/l+ IBA 4.0 mg/l gave the highest number of leaves (11.00) and maximum number of roots (3.60). The regenerated plantlet was acclimatized in natural pot and soil conditions. Finally, a convenient protocol technique has been established for in vitro regeneration of rucola, which can be used for large scale plantlet production.
Key Words: Phytohormone, Cotyledonary Nodes, Regeneration, Rucola, IBA and BA
The present investigation was carried out to study the in vitro regeneration in Rucola. Different concentrations and combinations of BA and IBA were used for regeneration of root and shoot in rucola. Cotyledonary nodes were used as explants. The highest number of shoots (4.60) and leaves (6.4) were recorded in treatment 4.0 mg/l BA with minimum (9.00) days. The maximum days (14.00) was noticed in a simple MS medium to regenerate lower no. of shoots. The treatment BA 4.0 mg/l produced the highest number of the main root (4.60) while it was lowest in the control treatment. The highest number of shoot (3.40) was noticed from the BA 4.0 mg/l + IBA 3.0 mg/l. The treatment BA 4.0 mg/l+ IBA 4.0 mg/l gave the highest number of leaves (11.00) and maximum number of roots (3.60). The regenerated plantlet was acclimatized in natural pot and soil conditions. Finally, a convenient protocol technique has been established for in vitro regeneration of rucola, which can be used for large scale plantlet production.
Key Words: Phytohormone, Cotyledonary Nodes, Regeneration, Rucola, IBA and BA
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Article Citations:
MLA
Afrin, S. et al. “Effect of phytohormone on shoot generation potentiality in rucola”. Journal of Bioscience and Agriculture Research, 24(01), (2020): 1990-1998.
APA
Afrin, S., Munsur, M. A. Z. A., Khan, M. S. I., Khatun, F. and Hoque, M. E. (2020). Effect of phytohormone on shoot generation potentiality in rucola. Journal of Bioscience and Agriculture Research, 23(02), 1990-1998.
Chicago
Afrin, S., Munsur, M. A. Z. A., Khan, M. S. I., Khatun, F. and Hoque, M. E. “Effect of phytohormone on shoot generation potentiality in rucola”. Journal of Bioscience and Agriculture Research, 24(01), (2020): 1990-1998.
Harvard
Afrin, S., Munsur, M. A. Z. A., Khan, M. S. I., Khatun, F. and Hoque, M. E. 2020. Effect of phytohormone on shoot generation potentiality in rucola. Journal of Bioscience and Agriculture Research, 24(01), pp. 1990-1998.
Vancouver
Afrin, S, Munsur, MAZA, Khan, MSI, Khatun, F and Hoque, ME. Effect of phytohormone on shoot generation potentiality in rucola. Journal of Bioscience and Agriculture Research, 2020 April 24(01), 1990-1998.
Afrin, S. et al. “Effect of phytohormone on shoot generation potentiality in rucola”. Journal of Bioscience and Agriculture Research, 24(01), (2020): 1990-1998.
APA
Afrin, S., Munsur, M. A. Z. A., Khan, M. S. I., Khatun, F. and Hoque, M. E. (2020). Effect of phytohormone on shoot generation potentiality in rucola. Journal of Bioscience and Agriculture Research, 23(02), 1990-1998.
Chicago
Afrin, S., Munsur, M. A. Z. A., Khan, M. S. I., Khatun, F. and Hoque, M. E. “Effect of phytohormone on shoot generation potentiality in rucola”. Journal of Bioscience and Agriculture Research, 24(01), (2020): 1990-1998.
Harvard
Afrin, S., Munsur, M. A. Z. A., Khan, M. S. I., Khatun, F. and Hoque, M. E. 2020. Effect of phytohormone on shoot generation potentiality in rucola. Journal of Bioscience and Agriculture Research, 24(01), pp. 1990-1998.
Vancouver
Afrin, S, Munsur, MAZA, Khan, MSI, Khatun, F and Hoque, ME. Effect of phytohormone on shoot generation potentiality in rucola. Journal of Bioscience and Agriculture Research, 2020 April 24(01), 1990-1998.
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