J. Biosci. Agric. Res. | Volume 24, Issue 01, 2006-2011 | https://doi.org/10.18801/jbar.240120.245
Article type: Research article | Received: 08.02.2020; Revised: 30.03.2020; First published online: 25 April 2020.
Article type: Research article | Received: 08.02.2020; Revised: 30.03.2020; First published online: 25 April 2020.
Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium)
Md. Shahidul Islam Khan 1, Fahima Khatun 1, Sadia Afrin 1, Md. Abu Zafur Al Munsur 2 and M. E. Hoque 1
1 Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka-1207
2 Department of Agricultural Extension, Khamarbari, Dhaka-1215, Bangladesh
✉ Corresponding author: almunsurdae@gmail.com (Munsur, MAZA)
1 Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka-1207
2 Department of Agricultural Extension, Khamarbari, Dhaka-1215, Bangladesh
✉ Corresponding author: almunsurdae@gmail.com (Munsur, MAZA)
Abstract
The present investigation was carried out to study the in vitro regeneration in chrysanthemum. MS medium supplemented with different concentrations and combinations of BA and IAA for regeneration and multiple shoot formations. Nodal segments were used as explants. The shoot induction percentage was highest (76%) in the treatment BA 2.0 mg/l+ IAA 1.0 mg/l. The same combination regenerated the highest number of shoot (3.40) with minimum (7.60) days. The maximum days (15.80) was noticed in a simple MS medium. The highest number of leaf (11.60) was observed in the treatment of 3.0 mg/l BA+ 1.0 mg/l IAA. The treatment BA 2.0 mg/l + IAA 1.0 mg/l produced the highest number of main root (4.20) while it was lowest in the control treatment. A robust and healthy plantlet was regenerated within 28 days of subculture in the treatment BA 2.0 mg/l + IAA 1.0 mg/l. The regenerated plantlet was acclimatized in natural pot and soil conditions. An approximate 75% plantlet was established in both systems. Finally, a convenient protocol technique has been established for in vitro regeneration of chrysanthemum which can be used for large scale plantlet production in chrysanthemum.
Key Words: Phytohormone, Regeneration, Chrysanthemum, BA, IAA, Shoot induction and Root Induction
The present investigation was carried out to study the in vitro regeneration in chrysanthemum. MS medium supplemented with different concentrations and combinations of BA and IAA for regeneration and multiple shoot formations. Nodal segments were used as explants. The shoot induction percentage was highest (76%) in the treatment BA 2.0 mg/l+ IAA 1.0 mg/l. The same combination regenerated the highest number of shoot (3.40) with minimum (7.60) days. The maximum days (15.80) was noticed in a simple MS medium. The highest number of leaf (11.60) was observed in the treatment of 3.0 mg/l BA+ 1.0 mg/l IAA. The treatment BA 2.0 mg/l + IAA 1.0 mg/l produced the highest number of main root (4.20) while it was lowest in the control treatment. A robust and healthy plantlet was regenerated within 28 days of subculture in the treatment BA 2.0 mg/l + IAA 1.0 mg/l. The regenerated plantlet was acclimatized in natural pot and soil conditions. An approximate 75% plantlet was established in both systems. Finally, a convenient protocol technique has been established for in vitro regeneration of chrysanthemum which can be used for large scale plantlet production in chrysanthemum.
Key Words: Phytohormone, Regeneration, Chrysanthemum, BA, IAA, Shoot induction and Root Induction
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MLA
Khan, M. S. I. et al. “Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium)”. Journal of Bioscience and Agriculture Research, 24(01), (2020): 2006-2011.
APA
Khan, M. S. I., Khatun, F., Afrin, S., Munsur, M. A. Z. A. and Hoque, M. E. (2020). Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium). Journal of Bioscience and Agriculture Research, 24(01), 2006-2011.
Chicago
Khan, M. S. I., Khatun, F., Afrin, S., Munsur, M. A. Z. A. and Hoque, M. E. “Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium)”. Journal of Bioscience and Agriculture Research, 24(01), (2020): 2006-2011.
Harvard
Khan, M. S. I., Khatun, F., Afrin, S., Munsur, M. A. Z. A. and Hoque, M. E. 2020. Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium). Journal of Bioscience and Agriculture Research, 24(01), pp. 2006-2011.
Vancouver
Khan, MSI, Khatun, F, Afrin, S, Munsur, MAZA and Hoque ME. Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium). Journal of Bioscience and Agriculture Research, 2020 April 24(01): 2006-2011.
Khan, M. S. I. et al. “Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium)”. Journal of Bioscience and Agriculture Research, 24(01), (2020): 2006-2011.
APA
Khan, M. S. I., Khatun, F., Afrin, S., Munsur, M. A. Z. A. and Hoque, M. E. (2020). Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium). Journal of Bioscience and Agriculture Research, 24(01), 2006-2011.
Chicago
Khan, M. S. I., Khatun, F., Afrin, S., Munsur, M. A. Z. A. and Hoque, M. E. “Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium)”. Journal of Bioscience and Agriculture Research, 24(01), (2020): 2006-2011.
Harvard
Khan, M. S. I., Khatun, F., Afrin, S., Munsur, M. A. Z. A. and Hoque, M. E. 2020. Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium). Journal of Bioscience and Agriculture Research, 24(01), pp. 2006-2011.
Vancouver
Khan, MSI, Khatun, F, Afrin, S, Munsur, MAZA and Hoque ME. Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium). Journal of Bioscience and Agriculture Research, 2020 April 24(01): 2006-2011.
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