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J. Biosci. Agric. Res. | Volume 24, Issue 02, 2020-2024 | https://doi.org/10.18801/jbar.240220.247
​Article type: Research article | Received: 17.05.2020; Revised: 26.05.2020; First published online: 30 May 2020.

Effect of hyaluronic acid on the organogenesis of an Oncidium cultivar

Shahrin Sharmin 1, Kazuhiko Shimasaki 2, A. F. M. Jamal Uddin 3 and Md. Manirul Alam 1
​1 The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8556 Japan.
2 Faculty of Agriculture and Marine Science, Kochi University B200 Monobe, Nankoku, Kochi 783-8502, Japan.
3 Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.

​✉  Corresponding author: [email protected]  (Sharmin S.).

Abstract
Protocorm-like bodies (PLBs) multiplication is one of the most preferable in vitro methods to increase the number of orchids in a short time. The current investigation was conducted to understand the impact of two types of hyaluronic acid (HA12 and HA20) using various concentrations on the regeneration of Oncidium Aloha ‘Iwanaga’. The highest number of PLBs were found at 0.1mg/l of HA12 (20.7/explant) and HA20 (23.1/explant) respectively. The highest number of shoots were found at 0.1mg/l of HA12 (2.1/explant) and HA20 (3.5/explant) respectively. The highest formation rate of PLBs and shoots was found in both hyaluronic acids at 0.1mg/l. No root formation was observed. The highest fresh weight was obtained with 0.1mg/l HA12 (195.5mg) and 0.1mg/l HA20 (212.3mg) respectively. So use of hyaluronic acid could be a potential source of PLB proliferation within short time in Oncidium Aloha ‘Iwanaga’.

Key Words: Orchid, Protocorm-like bodies (PLBs), In vitro micropropagation and Organogenesi

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Article Citations:

MLA
Sharmin, S. et al. “Effect of hyaluronic acid on the organogenesis of an Oncidium cultivar”. Journal of Bioscience and Agriculture Research, 24(02), (2020): 2020-2024.
 
APA
Sharmin, S., Shimasaki, K, Jamal Uddin, A. F. M. and Alam, MM (2020). Effect of hyaluronic acid on the organogenesis of an Oncidium cultivar. Journal of Bioscience and Agriculture Research, 24(02), 2020-2024.
 
Chicago
Sharmin, S., Shimasaki, K, Jamal Uddin, A. F.M. and Alam, M. M. “Effect of hyaluronic acid on the organogenesis of an Oncidium cultivar”. Journal of Bioscience and Agriculture Research, 24(02), (2020): 2020-2024.
 
Harvard
Sharmin, S., Shimasaki, K, Jamal Uddin, A. F. M. and Alam, M. M. 2020. Effect of hyaluronic acid on the organogenesis of an Oncidium cultivar. Journal of Bioscience and Agriculture Research, 24(02), pp. 2020-2024.
 
Vancouver
Sharmin, S, Shimasaki, K, Jamal Uddin, AFM and Alam, MM. Effect of hyaluronic acid on the organogenesis of an Oncidium cultivar. Journal of Bioscience and Agriculture Research, 2020 May 24(02), 2020-2024.

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