J. Biosci. Agric. Res. | Volume 24, Issue 02, 2025-2029 | https://doi.org/10.18801/jbar.240220.248
Article type: Research article | Received: 17.05.2020; Revised: 26.05.2020; First published online: 30 May 2020.
Article type: Research article | Received: 17.05.2020; Revised: 26.05.2020; First published online: 30 May 2020.
In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LED
Shahrin Sharmin 1, Kazuhiko Shimasaki 2 and A. F. M. Jamal Uddin 3
1 The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8556 Japan.
2 Faculty of Agriculture and Marine Science, Kochi University B200 Monobe, Nankoku, Kochi 783-8502, Japan.
3 Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.
✉ Corresponding author: [email protected] (Sharmin S)
1 The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8556 Japan.
2 Faculty of Agriculture and Marine Science, Kochi University B200 Monobe, Nankoku, Kochi 783-8502, Japan.
3 Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.
✉ Corresponding author: [email protected] (Sharmin S)
Abstract
Different developmental processes in plants are highly influenced by cytokinins. On the other hand, recently light emitting diode has garnered much reputation in plant tissue culture system due to its high energy efficiency and versatility. In the present study the effective concentration of 6-benzylaminopurine (BAP) on the organogenesis of Oncidium Aloha ‘Iwanaga’ was assessed and further evaluated under different LEDs. From the initial experiment, 0.1mg/l BAP was found to be the optimum concentration among others in terms of the average number of PLBs/explant and other parameters. The highest number of PLBs (28.1/explant) and shoots (2.0/explant) was obtained using 0.1mg/l BAP. Regarding root formation no response was observed under the treatment applied. In case of the LED treatments with 0.1mg/l BAP, the trial indicated that the number of PLBs increased under white (28.1/explant) and blue (26.2/explant) LED. Maximum shoot regeneration and fresh weight were observed under white LED compared to control. So, a combination of 0.1mg/l BAP with white LED could be proposed to be suitable for in vitro regeneration of Oncidium Aloha ‘Iwanaga’.
Key Words: Orchid, Protocorm-like bodies, Shoot, Micropropagation and Organogenesi
Different developmental processes in plants are highly influenced by cytokinins. On the other hand, recently light emitting diode has garnered much reputation in plant tissue culture system due to its high energy efficiency and versatility. In the present study the effective concentration of 6-benzylaminopurine (BAP) on the organogenesis of Oncidium Aloha ‘Iwanaga’ was assessed and further evaluated under different LEDs. From the initial experiment, 0.1mg/l BAP was found to be the optimum concentration among others in terms of the average number of PLBs/explant and other parameters. The highest number of PLBs (28.1/explant) and shoots (2.0/explant) was obtained using 0.1mg/l BAP. Regarding root formation no response was observed under the treatment applied. In case of the LED treatments with 0.1mg/l BAP, the trial indicated that the number of PLBs increased under white (28.1/explant) and blue (26.2/explant) LED. Maximum shoot regeneration and fresh weight were observed under white LED compared to control. So, a combination of 0.1mg/l BAP with white LED could be proposed to be suitable for in vitro regeneration of Oncidium Aloha ‘Iwanaga’.
Key Words: Orchid, Protocorm-like bodies, Shoot, Micropropagation and Organogenesi
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MLA
Sharmin, S. et al. “In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs”. Journal of Bioscience and Agriculture Research, 24(02), (2020): 2025-2029.
APA
Sharmin, S., Shimasaki, K. and Jamal Uddin, A. F. M. (2020). In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs. Journal of Bioscience and Agriculture Research, 24(02), 2025-2029.
Chicago
Sharmin, S., Shimasaki, K. and Jamal Uddin, A. F. M. “In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs”. Journal of Bioscience and Agriculture Research, 24(01), (2020) pp. 2025-2029.
Harvard
Sharmin, S., Shimasaki, K. and Jamal Uddin, A. F. M. 2020. In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs. Journal of Bioscience and Agriculture Research, 24(01), 2025-2029.
Vancouver
Sharmin, S, Shimasaki, K and Jamal Uddin, AFM. In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs. Journal of Bioscience and Agriculture Research, 2020 May 24(01), 2025-2029.
Sharmin, S. et al. “In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs”. Journal of Bioscience and Agriculture Research, 24(02), (2020): 2025-2029.
APA
Sharmin, S., Shimasaki, K. and Jamal Uddin, A. F. M. (2020). In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs. Journal of Bioscience and Agriculture Research, 24(02), 2025-2029.
Chicago
Sharmin, S., Shimasaki, K. and Jamal Uddin, A. F. M. “In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs”. Journal of Bioscience and Agriculture Research, 24(01), (2020) pp. 2025-2029.
Harvard
Sharmin, S., Shimasaki, K. and Jamal Uddin, A. F. M. 2020. In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs. Journal of Bioscience and Agriculture Research, 24(01), 2025-2029.
Vancouver
Sharmin, S, Shimasaki, K and Jamal Uddin, AFM. In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs. Journal of Bioscience and Agriculture Research, 2020 May 24(01), 2025-2029.
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