J. Biosci. Agric. Res. | Volume 24, Issue 02, 2025-2029 | https://doi.org/10.18801/jbar.240220.248
Article type: Research article | Received: 17.05.2020; Revised: 26.05.2020; First published online: 30 May 2020.
Article type: Research article | Received: 17.05.2020; Revised: 26.05.2020; First published online: 30 May 2020.
In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LED
Shahrin Sharmin 1, Kazuhiko Shimasaki 2 and A. F. M. Jamal Uddin 3
1 The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8556 Japan.
2 Faculty of Agriculture and Marine Science, Kochi University B200 Monobe, Nankoku, Kochi 783-8502, Japan.
3 Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.
✉ Corresponding author: shahrin.sharmin@gmail.com (Sharmin S)
1 The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8556 Japan.
2 Faculty of Agriculture and Marine Science, Kochi University B200 Monobe, Nankoku, Kochi 783-8502, Japan.
3 Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.
✉ Corresponding author: shahrin.sharmin@gmail.com (Sharmin S)
Abstract
Different developmental processes in plants are highly influenced by cytokinins. On the other hand, recently light emitting diode has garnered much reputation in plant tissue culture system due to its high energy efficiency and versatility. In the present study the effective concentration of 6-benzylaminopurine (BAP) on the organogenesis of Oncidium Aloha ‘Iwanaga’ was assessed and further evaluated under different LEDs. From the initial experiment, 0.1mg/l BAP was found to be the optimum concentration among others in terms of the average number of PLBs/explant and other parameters. The highest number of PLBs (28.1/explant) and shoots (2.0/explant) was obtained using 0.1mg/l BAP. Regarding root formation no response was observed under the treatment applied. In case of the LED treatments with 0.1mg/l BAP, the trial indicated that the number of PLBs increased under white (28.1/explant) and blue (26.2/explant) LED. Maximum shoot regeneration and fresh weight were observed under white LED compared to control. So, a combination of 0.1mg/l BAP with white LED could be proposed to be suitable for in vitro regeneration of Oncidium Aloha ‘Iwanaga’.
Key Words: Orchid, Protocorm-like bodies, Shoot, Micropropagation and Organogenesi
Different developmental processes in plants are highly influenced by cytokinins. On the other hand, recently light emitting diode has garnered much reputation in plant tissue culture system due to its high energy efficiency and versatility. In the present study the effective concentration of 6-benzylaminopurine (BAP) on the organogenesis of Oncidium Aloha ‘Iwanaga’ was assessed and further evaluated under different LEDs. From the initial experiment, 0.1mg/l BAP was found to be the optimum concentration among others in terms of the average number of PLBs/explant and other parameters. The highest number of PLBs (28.1/explant) and shoots (2.0/explant) was obtained using 0.1mg/l BAP. Regarding root formation no response was observed under the treatment applied. In case of the LED treatments with 0.1mg/l BAP, the trial indicated that the number of PLBs increased under white (28.1/explant) and blue (26.2/explant) LED. Maximum shoot regeneration and fresh weight were observed under white LED compared to control. So, a combination of 0.1mg/l BAP with white LED could be proposed to be suitable for in vitro regeneration of Oncidium Aloha ‘Iwanaga’.
Key Words: Orchid, Protocorm-like bodies, Shoot, Micropropagation and Organogenesi
Article Full-Text PDF:
| 248.24.02.2020_in_vitro_regeneration_of_an_oncidium_cultivar_using_different_concentrations_of_bap_under_different_leds.pdf |
Article Metrics:
|
Share This Article:
|
|
Article Citations:
MLA
Sharmin, S. et al. “In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs”. Journal of Bioscience and Agriculture Research, 24(02), (2020): 2025-2029.
APA
Sharmin, S., Shimasaki, K. and Jamal Uddin, A. F. M. (2020). In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs. Journal of Bioscience and Agriculture Research, 24(02), 2025-2029.
Chicago
Sharmin, S., Shimasaki, K. and Jamal Uddin, A. F. M. “In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs”. Journal of Bioscience and Agriculture Research, 24(01), (2020) pp. 2025-2029.
Harvard
Sharmin, S., Shimasaki, K. and Jamal Uddin, A. F. M. 2020. In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs. Journal of Bioscience and Agriculture Research, 24(01), 2025-2029.
Vancouver
Sharmin, S, Shimasaki, K and Jamal Uddin, AFM. In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs. Journal of Bioscience and Agriculture Research, 2020 May 24(01), 2025-2029.
Sharmin, S. et al. “In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs”. Journal of Bioscience and Agriculture Research, 24(02), (2020): 2025-2029.
APA
Sharmin, S., Shimasaki, K. and Jamal Uddin, A. F. M. (2020). In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs. Journal of Bioscience and Agriculture Research, 24(02), 2025-2029.
Chicago
Sharmin, S., Shimasaki, K. and Jamal Uddin, A. F. M. “In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs”. Journal of Bioscience and Agriculture Research, 24(01), (2020) pp. 2025-2029.
Harvard
Sharmin, S., Shimasaki, K. and Jamal Uddin, A. F. M. 2020. In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs. Journal of Bioscience and Agriculture Research, 24(01), 2025-2029.
Vancouver
Sharmin, S, Shimasaki, K and Jamal Uddin, AFM. In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs. Journal of Bioscience and Agriculture Research, 2020 May 24(01), 2025-2029.
References:
- Alam, M. M., Shimasaki, K., Habiba, S. U. and Jahan, N. (2020). Effect of alginate on protocorm like bodies (PLBs) formation of Dendrobium kingianum cultivar. Asian Journal of Crop, Soil Science and Plant Nutrition, 2(2), 62-67.
- Bello-Bello, J. J., Martínez-Estrada, E., Caamal-Velázquez, J. H. and Morales-Ramos, V. (2016). Effect of LED light quality on in vitro shoot proliferation and growth of vanilla (Vanilla planifolia Andrews). African Journal of Biotechnology, 15(8), 272-277. https://doi.org/10.5897/AJB2015.14662
- Chen, J. T. and Chang, W. C. (2000). Plant regeneration via embryo and shoot bud formation from flower-stalk explants of Oncidium Sweet Sugar. Plant Cell, Tissue and Organ Culture, 62(2), 95-100. https://doi.org/10.1023/A:1026591003553
- Chen, J. T. and Chang, W. C. (2003). Effect of GA3, ancymidol, cycocel and paclobutrazol on direct somatic embryogenesis of Oncidium in vitro. Plant Cell, Tissue and Organ Culture, 72(1), 105-108. https://doi.org/10.1023/A:1021235700751
- Chen, J. T., Chang, C. and Chang, W. C. (1999). Direct somatic embryogenesis on leaf explants of Oncidium Gower Ramsey and subsequent plant regeneration. Plant Cell Reports, 19(2), 143-149. https://doi.org/10.1007/s002990050724
- Habiba, S. U., Shimasaki, K., Ahasan, M. M. and Alam, M. M. (2014). Effect of benzylaminopurine (BA) and hyaluronic acid (HA) under white light emitting diode (LED) on organogenesis in protocorm-like bodies (PLBs) of Dendrobium kingianum. American-Eurasian Journal of Agricultural Environment Sciences, 14, 605–609.
- Kaewjampa, N. and Shimasaki, K. (2012). Effects of green LED lighting on organogenesis and superoxide dismutase (SOD) activities in protocorm-like bodies (PLBs) of Cymbidium cultured in vitro. Environmental Control in Biology, 50(3), 247-254.
- Kalimuthu, K., Senthilkumar, R. and Vijaya kumar, S. (2007). In vitro micropropagation, Oncidium sp. (Dancing Dolls). African Journal of Biotechnology, 6(10), 1171-1174.
- Kerbauy, G. B. (1984). Plant regeneration of Oncidium varicosum (Orchidaceae) by means of root tip culture. Plant Cell Reports, 3(1), 27-29. https://doi.org/10.1007/BF00270224
- Mondal, T., Aditya, S. and Banerjee, N. (2013). In vitro axillary shoot regeneration and direct protocorm-like body induction from axenic shoot tips of Doritis pulcherrima Lindl. Plant Tissue Culture and Biotechnology, 23(2), 251-261. https://doi.org/10.3329/ptcb.v23i2.17526
- Nasiruddin, K. M., Begum, R. and Yesmin, S. (2003). Protocorm-like bodies and plantlet regeneration from Dendrobium formosum leaf callus. Journal of Plant Sciences, 2(13), 955-957. https://doi.org/10.3923/ajps.2003.955.957
- Rahman, S. M. M., Islam, M. S., Sen, K. P. and Begum, F. (2005). In vitro propagation of Oncidium taka. Biotechnology, 4(3), 225-229. https://doi.org/10.3923/biotech.2005.225.229
- Samarfard, S., Kadir, M. A., Kadzimin, S. B., Ravanfar, S. and Saud, H. M. (2013). Genetic stability of in vitro multiplied Phalaenopsis gigantea protocorm-like bodies as affected by chitosan. Notulae Botanicae Horti Agrobotanici, 41(1), 177-183. https://doi.org/10.15835/nbha4118315
- Shimasaki, K. and Uemoto, S. (1990). Micropropagation of a terrestrial Cymbidium species using rhizomes developed from seeds and pseudobulbs. Plant Cell, Tissue and Organ Culture, 22, 237-244. https://doi.org/10.1007/BF00033642
- Siddiqui, M. W., Bhattacharjya, A., Chakraboty, I. and Dhua, R. S. (2011). 6-benzylaminopurine improves shelf life, organoleptic quality and health promoting compound of fresh cut broccoli florets. Journal of Scientific and Industrial Research, 70(6), 461-465.
- Sivakumar, G., Heo, J. W., Kozai, T. and Peak, K. Y. (2006). Effect of continuous or intermittent radiation on sweet potato plantlets in vitro. The Journal of Horticultural Science and Biotechnology, 81(3), 546-548. https://doi.org/10.1080/14620316.2006.11512101
- Solarte, M. E., Moreno, L. and Melgarejo, L. M. (2010). VI. Fotosíntesis y pigmentos vegetales. In: Melgarejo L. M. (ed). Experimentos en Fisiología Vegetal. Universidad Nacional de Colombia. pp: 107-122.
- Teixeira da Silva, J. A., Yam, T., Fukai, S., Nayak, N. and Tanaka, M. (2005). Establishment of optimum nutrient media for in vitro propagation of Cymbidium Sw. (Orchidaceae) using protocorm-like body segments. Propagation of Ornamental Plants, 5(3), 129-136.
- Wu, I. F., Chen, J. T. and Chang, W. C. (2004). Effect of auxins and cytokinins on embryo formation from root-derived callus of Oncidium ‘Gower Ramsey’. Plant Cell, Tissue and Organ Culture, 77, 107-109. https://doi.org/10.1023/B:TICU.0000016492.45075.a3
© 2020 The Authors. This article is freely available for anyone to read, share, download, print, permitted for unrestricted use and build upon, provided that the original author(s) and publisher are given due credit. All Published articles are distributed under the Creative Commons Attribution 4.0 International License.
Journal of Bioscience and Agriculture Research EISSN 2312-7945.