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J. Biosci. Agric. Res. | Volume 25, Issue 01, 2075-2084 | https://doi.org/10.18801/jbar.250120.254
​Article type: Research article | Received: 03.07.2020; Revised: 24.07.2020; First published online:  01 August 2020.

Molecular analysis of the isolated probiotic microorganisms from yoghurt samples, distributed in Khulna and Chittagong City of Bangladesh, using RAPD marker

Mithila Farjana, Khondoker Moazzem Hossain and Sana Mohammad Mahbubur Rahman
Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna University, Khulna-9208, Bangladesh.

✉  Corresponding author: xyz007.bio@gmail.com (Farjana, M.).

Abstract
Yogurt is among the most common dairy products being consumed around the world. During the last decade, probiotics have become an important trend and being used as a useful component regarding the development of functional foods and as substitutes of antibiotics. Probiotics are living, non-pathogenic microorganisms which has profuse beneficial health benefits. The study was aimed at isolation, DNA extraction and finally, molecular characterization using RAPD based PCR from 10 yogurt samples from Khulna and Chittagong Divisions of Bangladesh. Four random 10-mer RAPD primers viz. OPA 2, OPA 5, OPA 9, and OPA 18 were used for amplification. Among them, OPA 18 showed the highest number of amplified bands. The RAPD profiles were demonstrated by Neighbor-joining algorithms. According to the phylogenetic tree, the pair having lower genetic distance was closely related and made cluster. The analysis manifested that, the genetic distance value ranged from 0.14286 to 0.85714. Both genetic distances and dendrograms were determined by using PAUP software. Although most RAPD fragments were common to all ten isolates, where a lower number of polymorphic fragments (lower genetic diversity) were observed that allowed the clear distinction of the isolates. The results indicate that RAPD analysis could be a useful tool to study the molecular landscape on regional native probiotics of Bangladesh.
 
Key Words: Probiotics microorganisms, RAPD marker, PCR, Primer and Molecular landscape

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Farjana, M. et al “Molecular analysis of the isolated probiotic microorganisms from yoghurt samples, distributed in Khulna and Chittagong City of Bangladesh, using RAPD marker”. Journal of Bioscience and Agriculture Research, 25(01), (2020):2075-2084.
 
APA
Farjana, M., Hossain, K. M. and Rahman, S. M. M. (2020). Molecular analysis of the isolated probiotic microorganisms from yoghurt samples, distributed in Khulna and Chittagong City of Bangladesh, using RAPD marker. Journal of Bioscience and Agriculture Research, 25(01), 2075-2084.
 
Chicago
Farjana, M., Hossain, K. M. and Rahman, S. M. M. “Molecular analysis of the isolated probiotic microorganisms from yoghurt samples, distributed in Khulna and Chittagong City of Bangladesh, using RAPD marker”. Journal of Bioscience and Agriculture Research, 25(01), (2020): 2075-2084.
 
Harvard
Farjana, M., Hossain, K. M. and Rahman, S. M. M. 2020. Molecular analysis of the isolated probiotic microorganisms from yoghurt samples, distributed in Khulna and Chittagong City of Bangladesh, using RAPD marker. Journal of Bioscience and Agriculture Research, 25(01), pp. 2075-2084.
 
Vancouver
Farjana, M, Hossain, KM and Rahman, SMM. Molecular analysis of the isolated probiotic microorganisms from yoghurt samples, distributed in Khulna and Chittagong City of Bangladesh, using RAPD marker. Journal of Bioscience and Agriculture Research, 2020 August 25(01), 2075-2084.

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