J. Biosci. Agric. Res. | Volume 26, Issue 01, 2137-2142 | https://doi.org/10.18801/jbar.260120.261
Article type: Research article | Received: 01.10.2020; Revised: 22.10.2020; First published online: 30 October 2020.
Article type: Research article | Received: 01.10.2020; Revised: 22.10.2020; First published online: 30 October 2020.
Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro
Alam Md Meskatul 1, Kazuhiko Shimasaki 2 and Sultana Umma Habiba 3
1 The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8556, Japan.
2 Faculty of Agriculture and Marine Science, Kochi University, Monobe B200, Nankoku, Kochi 783-8502, Japan.
3 618-1, Tateda, Nankoku, Kochi 783-0091, Japan.
✉ Corresponding author: [email protected] (Meskatul, A.M.).
1 The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8556, Japan.
2 Faculty of Agriculture and Marine Science, Kochi University, Monobe B200, Nankoku, Kochi 783-8502, Japan.
3 618-1, Tateda, Nankoku, Kochi 783-0091, Japan.
✉ Corresponding author: [email protected] (Meskatul, A.M.).
Abstract
Different types of bio-polysaccharide play a vital role in the growth of PLBs cultured in vitro. In this study, to we investigated the potential impacts of two bio-polymers,: hyaluronic acid (HA9) and sodium alginate (ALG) on the organogenesis of protocorm-like bodies (PLBs) in Phalaenopsis under white LED lights. PLBs of Phalaenopsis ‘Fmk02010’ were explanted on modified MS medium with different concentrations of HA and (ALG). The highest average number of PLBs per explant (24.6) was recorded for ALG alone at a concentration of 0.01mg/L, and the fresh weight was also highest at the same concentration. The combination of 0.01mg/L ALG and 0.01mg/L HA also resulted in a large number of PLBs (23.8) and high fresh weight. As opposed to, the highest number of shoots /explant (3.6) was observed at the treatment of the combination of 1mg/L ALG and 10mg/L HA. This study shows that the application of ALG and HA alone, and in combination, at low concentrations, increased the average number of PLBs and the amount of fresh weight, but shoot formation was higher at a high concentration compared with control.
Key Words: Phalaenopsis, Protocorm-like body (PLB), Hyaluronic acid (HA) and Sodium alginate (ALG).
Different types of bio-polysaccharide play a vital role in the growth of PLBs cultured in vitro. In this study, to we investigated the potential impacts of two bio-polymers,: hyaluronic acid (HA9) and sodium alginate (ALG) on the organogenesis of protocorm-like bodies (PLBs) in Phalaenopsis under white LED lights. PLBs of Phalaenopsis ‘Fmk02010’ were explanted on modified MS medium with different concentrations of HA and (ALG). The highest average number of PLBs per explant (24.6) was recorded for ALG alone at a concentration of 0.01mg/L, and the fresh weight was also highest at the same concentration. The combination of 0.01mg/L ALG and 0.01mg/L HA also resulted in a large number of PLBs (23.8) and high fresh weight. As opposed to, the highest number of shoots /explant (3.6) was observed at the treatment of the combination of 1mg/L ALG and 10mg/L HA. This study shows that the application of ALG and HA alone, and in combination, at low concentrations, increased the average number of PLBs and the amount of fresh weight, but shoot formation was higher at a high concentration compared with control.
Key Words: Phalaenopsis, Protocorm-like body (PLB), Hyaluronic acid (HA) and Sodium alginate (ALG).
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MLA
Meskatul, A. M. et al. “Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro”. Journal of Bioscience and Agriculture Research, 26(01), (2020): 2137-2142.
APA
Meskatul, A. M., Shimasaki, K. and Habiba, S. U. (2020). Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro. Journal of Bioscience and Agriculture Research, 26(01), 2137-2142.
Chicago
Meskatul, A. M., Shimasaki, K. and Habiba, S. U. “Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro”. Journal of Bioscience and Agriculture Research, 26(01), (2020): 2137-2142.
Harvard
Meskatul, A. M., Shimasaki, K. and Habiba, S. U. 2020. Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro. Journal of Bioscience and Agriculture Research, 26(01), pp. 2137-2142.
Vancouver
Meskatul, AM, Shimasaki, K and Habiba, SU. Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro. Journal of Bioscience and Agriculture Research, 2020 October 26(01): 2137-2142.
Meskatul, A. M. et al. “Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro”. Journal of Bioscience and Agriculture Research, 26(01), (2020): 2137-2142.
APA
Meskatul, A. M., Shimasaki, K. and Habiba, S. U. (2020). Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro. Journal of Bioscience and Agriculture Research, 26(01), 2137-2142.
Chicago
Meskatul, A. M., Shimasaki, K. and Habiba, S. U. “Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro”. Journal of Bioscience and Agriculture Research, 26(01), (2020): 2137-2142.
Harvard
Meskatul, A. M., Shimasaki, K. and Habiba, S. U. 2020. Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro. Journal of Bioscience and Agriculture Research, 26(01), pp. 2137-2142.
Vancouver
Meskatul, AM, Shimasaki, K and Habiba, SU. Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro. Journal of Bioscience and Agriculture Research, 2020 October 26(01): 2137-2142.
References:
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