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​Journal of Bioscience and Agriculture Research

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J. Biosci. Agric. Res. | Volume 26, Issue 01, 2137-2142 | https://doi.org/10.18801/jbar.260120.261
​Article type: Research article | Received: 01.10.2020; Revised: 22.10.2020; First published online: 30 October 2020.

Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro

Alam Md Meskatul 1, Kazuhiko Shimasaki 2 and Sultana Umma Habiba 3
1 The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8556, Japan.
2 Faculty of Agriculture and Marine Science, Kochi University, Monobe B200, Nankoku, Kochi 783-8502, Japan.
3 618-1, Tateda, Nankoku, Kochi 783-0091, Japan.

✉  Corresponding author: alam_ju87@yahoo.com (Meskatul, A.M.).
Abstract
Different types of bio-polysaccharide play a vital role in the growth of PLBs cultured in vitro. In this study, to we investigated the potential impacts of two bio-polymers,: hyaluronic acid (HA9) and sodium alginate (ALG) on the organogenesis of protocorm-like bodies (PLBs) in Phalaenopsis under white LED lights. PLBs of Phalaenopsis ‘Fmk02010’ were explanted on modified MS medium with different concentrations of HA and (ALG). The highest average number of PLBs per explant (24.6) was recorded for ALG alone at a concentration of 0.01mg/L, and the fresh weight was also highest at the same concentration. The combination of 0.01mg/L ALG and 0.01mg/L HA also resulted in a large number of PLBs (23.8) and high fresh weight. As opposed to, the highest number of shoots /explant (3.6) was observed at the treatment of the combination of 1mg/L ALG and 10mg/L HA. This study shows that the application of ALG and HA alone, and in combination, at low concentrations, increased the average number of PLBs and the amount of fresh weight, but shoot formation was higher at a high concentration compared with control.

Key Words: Phalaenopsis, Protocorm-like body (PLB), Hyaluronic acid (HA) and Sodium alginate (ALG).
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MLA
Meskatul, A. M. et al. “Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro”. Journal of Bioscience and Agriculture Research, 26(01), (2020): 2137-2142.
 
APA
Meskatul, A. M., Shimasaki, K. and Habiba, S. U. (2020). Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro. Journal of Bioscience and Agriculture Research, 26(01), 2137-2142.
 
Chicago
Meskatul, A. M., Shimasaki, K. and Habiba, S. U. “Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro”. Journal of Bioscience and Agriculture Research, 26(01), (2020): 2137-2142.
 
Harvard
Meskatul, A. M., Shimasaki, K. and Habiba, S. U. 2020. Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro. Journal of Bioscience and Agriculture Research, 26(01), pp. 2137-2142.
 
Vancouver
Meskatul, AM, Shimasaki, K and Habiba, SU. Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro. Journal of Bioscience and Agriculture Research, 2020 October 26(01): 2137-2142.
References:
  1. Alam, M. M., Shimasaki, K., Habiba, S. U. and Jahan, N. (2020). Effect of alginate on protocorm like bodies (PLBs) formation of Dendrobium kingianum cultivar. Asian Journal of Crop Soil Science and Plant Nutrition, 02(02), 62-67. https://doi.org/10.18801/ajcsp.020220.09
  2. Arditti, J. R. (1993). Micropropagation of orchids. John Wiley and Sons, New York.USA.
  3. Chen, Y. C., Chang, C. and Chang, W. C. (2000). A reliable protocol for plant Regeneration from callus culture of Phalaenopsis. In Vitro Cellular & Developmental Biology. 36, 420-423.
  4. Habiba, S. U., Shimasaki, K., Ahasan, M. M. and Uddin, A. F. M. J. (2017). Effect of two bio polysaccharides on organogenesis in PLBs of Dendrobium kingianum in vitro. Acta Horticulturae, 1167, 127-132. https://doi.org/10.17660/ActaHortic.2017.1167.19
  5. Habiba, S. U., Shimasaki, K., Ahasan, M. M. and Alam, M. M. (2014). Effect of 6-benzylaminopurine (BA) and hyaluronic acid (HA) under white light emitting diode (LED) on organogenesis in protocorm-like bodies (PLBs) of Dendrobium kingianum. America-Eurasian Journal of Agricultural Environmental Sciences, 14, 605-609.
  6. Ichihashi, S. (1992). Micropropagation of Phalaenopsis through the culture of lateral buds from young flower stalks. Lindleyana, 7, 208–215.
  7. Kazi, S. S., Kazi, M. H., Kazi, M. H. H., Mehraj, Shimasaki, K. and Habiba, S. U. (2015). Effect of Hyaluronic Acid (HA) on organogenesis in Protocorm-Like Bodies (PLBs) of Phalaenopsis ‘Fmk02010’ cultured in vitro. American-Eurasian Journal of Agricultural & Environmental Sciences, 15(9), 1721-1724.
  8. Meyer, K. and Palmer, J. W. (1934). The polysaccharide of the vitreous humor. Journal of Biological Chemistry, 107, 629-634.
  9. Milelle, E., Brescia, E., Massaro, C., Ramires, P. A., Miglietta, M. R., Fiori, V. and Aversa P. (2002). Physico-chemical properties and degradability of non-woven hyaluronan benzylic esters as tissue engineering scaffolds, Biomaterials-23(4), 1053-1063.
  10. Nahar, S. J., and Shimasaki, K. (2012). Effect of hyaluronic acid on organogenesis in  protocorm-like bodies (PLBs) of some Cymbidium species in vitro. International Symposium on Orchids and ornamental Plants. Chiang Mai, Thailand, 9th-12nd January 2012.
  11. Park, K., Paul, D., Kim, E. and Kloepper, J. W. (2008). Hyaluronic acid of Streptococcus sp. as a potent elicitor for induction of systemic resistance against plant diseases. World Journal of Microbiology and Biotechnology, 24, 1153–1158. https://doi.org/10.1007/s11274-007-9587-0
  12. Park, S. Y., Murthy, H. N. and Paek, K. Y. (2002).  Rapid propagation of Phalaenopsis from floral stalk-derived leaves. In Vitro Cellular & Developmental Biology, 38, 168–172.
  13. Shimasaki Kazuhiko. and Uemoto, S. (1990). Micropropagation of aterrestrial Cymbidium species using rhizomes developed from seeds and pseudo bulbs. Plant Cell Tissue and Organ Culture, 22, 237–244, https://doi.org/10.1007/BF00033642

© 2020 The Authors. This article is freely available for anyone to read, share, download, print, permitted for unrestricted use and build upon, provided that the original author(s) and publisher are given due credit. All Published articles are distributed under the Creative Commons Attribution 4.0 International License.
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