​Journal of Bioscience and Agriculture Research

You are here: Home>JBAR Journal​>JBAR Archive>Article Page: jbar-260120-265.html

submit Manuscript

J. Biosci. Agric. Res. | Volume 26, Issue 01, 2170-2176 | https://doi.org/10.18801/jbar.260120.265
​Article type: Research article | Received: 22.10.2020; Revised: 17.11.2020; First published online: 30 November 2020.

Effects of sucrose and trehalose on growth and development of protocorm-like bodies (PLBs) on two Dendrobium cultivars under different lights

Md. Manirul Alam 1, Kazuhiko Shimasaki 2 and Alam Md Meskatul 1
1 The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8556, Japan.
2 Faculty of Agriculture and Marine Science, Kochi University, Monobe B200, Nankoku, Kochi 783-8502, Japan.

✉  Corresponding author: [email protected] (Alam M.M.).

Abstract
The objective of this study was to identify effective carbon sources for the in vitro propagation of PLBs in Dendrobium cultivars Dendrobium kingianum ‘Hallelujah’ and Dendrobium k. Jonathan’s Glory ‘Dark Joy’. In this study, we used two types of carbon sources to culture the cultivars under five different LED lights. For, both carbon sources the highest numbers of PLBs were obtained with the green LEDs compared with the other LED lights. For Dendrobium kingianum ‘Hallelujah’ the trehalose supplemented medium produced the highest number of PLBs (13.8/explant) and the maximum fresh weight (0.45g/explant) under green LEDs. On the other hand, for Dendrobium k. Jonathan’s Glory ‘Dark Joy’, the sucrose supplemented medium produced the highest number of PLBs under green LED (8.0/explant, fresh weight 0.22g/explants) and the trehalose supplemented medium produced the maximum number of PLBs under white LEDs (8.1/explants, fresh weight 0.23g/explants). The sucrose was the most relevant carbon source for the in vitro organogenesis of Dendrobium k. Jonathan’s Glory ‘Dark Joy’, while trehalose was best under white LEDs.

Key Words: Dendrobium, protocorm-like bodies (PLBs), Light emitting diodes (LEDs)

Article Full-Text PDF:

265.26.01.2020_effects_of_sucrose_and_trehalose_on_growth_and_development_of_protocorm-like_bodies__plbs__on_two_dendrobium_cultivars_under_different_lights.pdf
File Size:	828 kb
File Type:	pdf

Download File

---

Article Metrics:

---

Share This Article:

Article Citations:

MLA
Alam, M. M. et al. “Effects of sucrose and trehalose on growth and development of protocorm-like bodies (PLBs) on two Dendrobium cultivars under different lights”. Journal of Bioscience and Agriculture Research, 26(01), (2020): 2170-2176.
 
APA
Alam, M. M., Shimasaki, K. and Meskatul, A. M. (2020). Effects of sucrose and trehalose on growth and development of protocorm-like bodies (PLBs) on two Dendrobium cultivars under different lights. Journal of Bioscience and Agriculture Research, 26(01), 2170-2176.
 
Chicago
Alam, M. M., Shimasaki, K. and Meskatul, A. M. “Effects of sucrose and trehalose on growth and development of protocorm-like bodies (PLBs) on two Dendrobium cultivars under different lights”. Journal of Bioscience and Agriculture Research, 26(01), (2020): 2170-2176.
 
Harvard
Alam, M. M., Shimasaki, K. and Meskatul, A. M. 2020. Effects of sucrose and trehalose on growth and development of protocorm-like bodies (PLBs) on two Dendrobium cultivars under different lights. Journal of Bioscience and Agriculture Research, 26(01), pp. 2170-2176.
 
Vancouver
Alam, MM, Shimasaki, K and Meskatul, AM. Effects of sucrose and trehalose on growth and development of protocorm-like bodies (PLBs) on two Dendrobium cultivars under different lights. Journal of Bioscience and Agriculture Research, 2020 November 26(01): 2170-2176.

References:

1. Al-Khateeb, A. A. (2008). Regulation of in vitro bud formation of date palm (Phoenix dactylifera L.) cv. Khanezi by different carbon sources. Bioresource Technology, 99, 6550-6555. https://doi.org/10.1016/j.biortech.2007.11.070
   
2. Borisjuk, L., Walenta, S., Rollerschek, H., Mueller-Klieser, W., Wobus, U. and Weber, H. (2002). Spatial analysis of plant metabolism: Sucrose imaging within Vicia faba in cotyledons reveals specific developmental patterns. The Plant Journal, 29(4), 521-530.
   
3. Capellades, M., Lemeur, R. and Debergh, P. (1991). Effects of sucrose on starch accumulation and rate of photosynthesis in Rosa cultured in vitro. Plant Cell, Tissue and Organ Culture, 25(1), 21-26. https://doi.org/10.1007/BF00033908
   
4. Chugh, S., Guha, S. and Rao, U. (2009). Micropropagation of orchids: A review on the potential of different explants. Scientia Horticulturae, 122(4), 507-520.  https://doi.org/10.1016/j.scienta.2009.07.016
   
5. Ernest, R. (1967). Effect of carbohydrate selection on the growth rate of freshly germinated Phalaenopsis and Dendrobium seed. American Orchid Society Bulletin, 36, 1068–1073.
   
6. Hew, C. S. and Mah, T. C. (1989). Sugar uptake and invertase activity in Dendrobium tissues. New Phytologist, 111, 167–171 https://doi.org/10.1111/j.1469-8137.1989.tb00678.x
   
7. Homma, T., Anzai, T., Matsuo, K., Kanemitsu, N. and Satoh, H. (2009). Effects of continuous irradiation of LED light on growth of young tea plants. Acta Horticulturae, 907, 233-236. https://doi.org/10.17660/ActaHortic.2011.907.35
   
8. Kane, M. E. (2011). Propagation by shoot culture. In: Trigiano, R. N. and Gray, D. J. (Eds) Plant Tissue Culture, Development and Biotechnology. Boca Raton: CRC Press, 181-192.
   
9. Kudo, R., Ishida, Y. and Yamamoto, K. (2009). Effects of green light irradiation on induction of diseases resistance in plants. 6th International Symposium on Light in Horticulture. Acta Horticulturae. 907, 251-254. https://doi.org/10.17660/ActaHortic.2011.907.39
   
10. Lin, Y., Li, J., Li, B., He, T. and Chun, Z. (2011). Effects of light quality on growth and development of protocorm-like bodies of Dendrobium officinale in vitro. Plant Cell, Tissue and Organ Culture, 105, 329-335. https://doi.org/10.1007/s11240-010-9871-9
    
11. Liu, T. H. A., Lin, J. J. and Wu. R. Y. (2006). The effects of using trehalose as a carbon source on the proliferation of Phalaenopsis and Doritaenopsis protocorm-like bodies. Plant Cell, Tissue and Organ Culture, 86, 125-129. https://doi.org/10.1007/s11240-006-9092-4
    
12. Luo, J. P., Wawrosch, C. and Kopp, B. (2009). Enhanced micropropagation of Dendrobium huoshanense C.Z. Tang et S.J. Cheng through protocorm-like bodies: The effect of cytokinins, carbohydrate sources and cold pretreatment. Scientia Horticulturae, 123(2), 258-262.  https://doi.org/10.1016/j.scienta.2009.08.008
    
13. Luo, J. P., Zha, X. Q. and Jiang, S. T. (2003). Suspension culture of protocorm-like bodies from the endangered medicinal plant Dendrobium huoshanenese. China Journal of Chinese Material Medicine, 28(7), 611-614.
    
14. Manivannan, A., Soundara rajan, P., Halimah, N. and Ko, C. H. (2015). Blue LED light enhances growth, phytochemical contents, and antioxidant enzyme activities of Rehmannia glutinosa cultured in vitro. Horticulture, Environment, and Biotechnology, 56, 105–113.  https://doi.org/10.1007/s13580-015-0114-1
    
15. Murdad, R., Latip, M. A., Aziz, Z. A. and Ripin, R. (2010). Effects of carbon source and potato homogenate on in vitro growth and development of Sabah’s endangered orchid: Phalaenopsis gigantea. Asia-Pacific Journal of Molecular Biology and Biotechnology, 18(1), 199- 202.
    
16. Paiva-Neto, V. B. and Otoni, W. C. (2003). Carbon sources and their osmotic potential in plant tissue culture: Does it matter?. Science Horticulture, 97, 193-202. https://doi.org/10.1016/S0304-4238(02)00231-5
    
17. Shimasaki, K., Tanibuchi, Y. and Fukumoto, M. (2003). The effects of sucrose and trehalose on organogenesis in protocorm-like body and rhizome of Cymbidium species. Japanese Society of High Technology in Agriculture, 15(3), 130-134. (in Japanese, English summary) https://doi.org/10.2525/jshita.15.130
    
18. Shimasaki, K. and Uemoto S. (1990). Micropropagation of Cypripedium macranthos var. rebunense through protocorm-like bodies derived from mature seeds. Plant Cell, Tissue and Organ Culture, 78(3), 273-276. https://doi.org/10.1023/B:TICU.0000025641.49000.b5
    
19. Smith, S. E. (1973). Asymbiotic germination of orchid seeds on carbohydrates of fungal origin. New Phytologist, 72, 497–499. https://doi.org/10.1111/j.1469-8137.1973.tb04400.x
    
20. Taiz, L. and Zeiger, E. (1991). Plant Physiology, 1st edition. Benjamin-Cumings Publishing Co.: New York, NY, USA.
    
21. Tokuhara, K. and Mii, M. (2003). Highly efficient somatic embryogenesis from cell suspension cultures of Phalaenopsis orchids by adjusting carbohydrate sources In Vitro Cell. Dev.  Biology Plant, 39, 635–639. https://doi.org/10.1079/IVP2003466
    
22. Topchiy, N. M., Sytnik, S. K., Syvash, O. O. and Zolotareva, O. K. (2005). The effect of additional red irradiation on the photosynthetic apparatus of Pisum sativum. Photosynthetica, 43, 451–456.  https://doi.org/10.1007/s11099-005-0072-4
    
23. Xu, Z. G., Cui, J. and Di, X. R. (2009). Effects of different spectral energy distribution on tissue culture of Oncidium in vitro. International Journal of Automation Computing, 31, 45–50.
    
24. Zha, X. Q., Luo, J, P. Jiang, S. T. and Wan, J. H. (2007). Enhancement of polysaccharide production in suspension cultures of protocorm-like bodies from Dendrobium huoshanense by optimization of medium compositions and feeding sucrose. Process Biochemistry, 42(3), 344-351. https://doi.org/10.1016/j.procbio.2006.09.008
    

---

© 2020 The Authors. This article is freely available for anyone to read, share, download, print, permitted for unrestricted use and build upon, provided that the original author(s) and publisher are given due credit. All Published articles are distributed under the Creative Commons Attribution 4.0 International License.

​Require any changes or update in this article? Please contact from HERE.

Journal of Bioscience and Agriculture Research EISSN ​2312-7945.