J. Biosci. Agric. Res. | Volume 26, Issue 02, 2211-2216 | https://doi.org/10.18801/jbar.260220.270
Article type: Research article | Received: 19.10.2020; Revised: 20.12.2020; First published online: 30 December 2020.
Article type: Research article | Received: 19.10.2020; Revised: 20.12.2020; First published online: 30 December 2020.
Isolation, identification and characterization of bacteria from Lawachara National Park, Moulovibazar, Bangladesh
Rajnee Hasan, M. N. H. Rony, Mohammad Saiful Alam Sarker, Md. Zablul Tareq and Rasel Ahmed
Basic and Applied Research on Jute Project, Bangladesh Jute Research Institute, Dhaka, Bangladesh.
✉ Corresponding author: [email protected] (Hasan, R.)
Basic and Applied Research on Jute Project, Bangladesh Jute Research Institute, Dhaka, Bangladesh.
✉ Corresponding author: [email protected] (Hasan, R.)
Abstract
Bacteria are essential elements of natural environments. As bacteria are the key critical components of food webs and nutrient cycles, they contribute to ecosystem functioning via mutualists and pathogens for larger species. The present study has provided substantial grounds to confirm that microbial communities present in natural environments are much more diverse. Here, we tend to study a singular environment Lawachara National Park. Total 125 bacterial strains were isolated using serial dilution method. Thirteen unique colonies were selected, cultured and characterized by gram staining and biochemical tests. Based on morphological, biochemical, 16S rDNA gene sequencing and phylogeny analysis revealed that the isolates were identified as Staphylococcus aureus, Micrococcus luteus, Bacillus cereus, Bacillus subtilis, Bacillus thuringensis, Serratia marcescens, Aeromonas hydrophila, Enterobacter cowanii, Acidobacterium capsulatum, Klebsiella pneumonia, Pseudomonas aeruginosa, Escherichia coli and Proteus mirabilis. This study serves as a baseline survey of bacterial diversity in the Lawachara National Park.
Key Words: Bacterial diversity, Biochemical test, 16S rDNA gene sequencing and Phylogeny
Bacteria are essential elements of natural environments. As bacteria are the key critical components of food webs and nutrient cycles, they contribute to ecosystem functioning via mutualists and pathogens for larger species. The present study has provided substantial grounds to confirm that microbial communities present in natural environments are much more diverse. Here, we tend to study a singular environment Lawachara National Park. Total 125 bacterial strains were isolated using serial dilution method. Thirteen unique colonies were selected, cultured and characterized by gram staining and biochemical tests. Based on morphological, biochemical, 16S rDNA gene sequencing and phylogeny analysis revealed that the isolates were identified as Staphylococcus aureus, Micrococcus luteus, Bacillus cereus, Bacillus subtilis, Bacillus thuringensis, Serratia marcescens, Aeromonas hydrophila, Enterobacter cowanii, Acidobacterium capsulatum, Klebsiella pneumonia, Pseudomonas aeruginosa, Escherichia coli and Proteus mirabilis. This study serves as a baseline survey of bacterial diversity in the Lawachara National Park.
Key Words: Bacterial diversity, Biochemical test, 16S rDNA gene sequencing and Phylogeny
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Article Citations:
MLA
Hasan, R. et al. “Isolation, identification and characterization of bacteria from Lawachara National Park, Moulovibazar, Bangladesh”. Journal of Bioscience and Agriculture Research, 26(02), (2020): 2211-2216.
APA
Hasan, R., Rony, M. N. H., Sarker, M. S. A., Tareq, M. Z. and Ahmed, R. (2020). Isolation, identification and characterization of bacteria from Lawachara National Park, Moulovibazar, Bangladesh. Journal of Bioscience and Agriculture Research, 26(02), 2211-2216.
Chicago
Hasan, R., Rony, M. N. H., Sarker, M. S. A., Tareq, M. Z. and Ahmed, R. “Isolation, identification and characterization of bacteria from Lawachara National Park, Moulovibazar, Bangladesh”. Journal of Bioscience and Agriculture Research, 26(02), (2020): 2211-2216.
Harvard
Hasan, R., Rony, M. N. H., Sarker, M. S. A., Tareq, M. Z. and Ahmed, R. 2020. Isolation, identification and characterization of bacteria from Lawachara National Park, Moulovibazar, Bangladesh. Journal of Bioscience and Agriculture Research, 26(02), pp. 2211-2216.
Vancouver
Hasan, R, Rony, MNH, Sarker, MSA, Tareq, MZ and Ahmed, R. Isolation, identification and characterization of bacteria from Lawachara National Park, Moulovibazar, Bangladesh. Journal of Bioscience and Agriculture Research, 2020 December 26(02): 2211-2216.
Hasan, R. et al. “Isolation, identification and characterization of bacteria from Lawachara National Park, Moulovibazar, Bangladesh”. Journal of Bioscience and Agriculture Research, 26(02), (2020): 2211-2216.
APA
Hasan, R., Rony, M. N. H., Sarker, M. S. A., Tareq, M. Z. and Ahmed, R. (2020). Isolation, identification and characterization of bacteria from Lawachara National Park, Moulovibazar, Bangladesh. Journal of Bioscience and Agriculture Research, 26(02), 2211-2216.
Chicago
Hasan, R., Rony, M. N. H., Sarker, M. S. A., Tareq, M. Z. and Ahmed, R. “Isolation, identification and characterization of bacteria from Lawachara National Park, Moulovibazar, Bangladesh”. Journal of Bioscience and Agriculture Research, 26(02), (2020): 2211-2216.
Harvard
Hasan, R., Rony, M. N. H., Sarker, M. S. A., Tareq, M. Z. and Ahmed, R. 2020. Isolation, identification and characterization of bacteria from Lawachara National Park, Moulovibazar, Bangladesh. Journal of Bioscience and Agriculture Research, 26(02), pp. 2211-2216.
Vancouver
Hasan, R, Rony, MNH, Sarker, MSA, Tareq, MZ and Ahmed, R. Isolation, identification and characterization of bacteria from Lawachara National Park, Moulovibazar, Bangladesh. Journal of Bioscience and Agriculture Research, 2020 December 26(02): 2211-2216.
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