J. Biosci. Agric. Res. | Volume 29, Issue 01, 2433-2442 | https://doi.org/10.18801/jbar.290122.295
Article type: Research article | Received: 10.06.2022; Revised: 22.07.2022; First published online: 30 August, 2022.
Article type: Research article | Received: 10.06.2022; Revised: 22.07.2022; First published online: 30 August, 2022.
Growth performance of Spirulina platensis in supernatant of digested rotten guava
Md. Saiful Islam 1, Md. Ahsan Bin Habib 1, Md. Hashibur Rahman 2 and Md. Arifuzzaman 3
1 Dept. of Aquaculture, Bangladesh Agricultural University, Mymensingh, Bangladesh
2 Bangladesh Fisheries Research Institute, Headquarters, Mymensingh, Bangladesh
3 Bangladesh Fisheries Research Institute, Shrimp Research Station, Bagerhat, Bangladesh
✉ Corresponding author: [email protected] (Rahman MH).
1 Dept. of Aquaculture, Bangladesh Agricultural University, Mymensingh, Bangladesh
2 Bangladesh Fisheries Research Institute, Headquarters, Mymensingh, Bangladesh
3 Bangladesh Fisheries Research Institute, Shrimp Research Station, Bagerhat, Bangladesh
✉ Corresponding author: [email protected] (Rahman MH).
Abstract
This study was performed to evaluate the culture potentiality and growth performance of Spirulina (Spirulina platensis) in supernatant of three different concentrations of digested rotten guava (DRG) and Kosaric Medium (KM) as control. The study consisted of three different concentrations of rotten guavas, such as 20 (T1), 40 (T2) and 60 (T3) were allowed to digest under aeration. After 34 days, three replications showed the reddish white-colored supernatant screened and taken in a 2.0 L conical flask. Then, Spirulina was inoculated to grow in these three digested rotten guava media (DRGM) with the addition of micronutrients and KM and 9.0 g/L NaHCO3 for 14 days. The cell weight of Spirulina was attained at a maximum of 12.43±0.10 mg/L (dry wt. basis) in KM, followed by 0.818 ± 0.002, 0.815 ± 0.0014 and 0.809± 0.0011 mg/L in supernatant of 60, 20 and 40% DRGM, respectively on the 10th day of culture. A similar trend was also observed in the case of specific growth rates (based on cell weight and chlorophyll a) and total biomass (mg/L) of Spirulina. Cell weight of Spirulina had a highly significant (P<0.01) correlation with the chlorophyll a content (r= 0.746) and total biomass (r= 0.742) of Spirulina. The results on the growth performance showed that Spirulina grown in supernatant of 60% DRGM was significantly (P<0.01) higher (T3) than that of Spirulina grown in supernatant of 20% (T1) and 40% DRGM (T2).
Key Words: Culture potentiality, Growth performance, Spirulina platensis, Digested Rotten Guava Media (DRGM)
This study was performed to evaluate the culture potentiality and growth performance of Spirulina (Spirulina platensis) in supernatant of three different concentrations of digested rotten guava (DRG) and Kosaric Medium (KM) as control. The study consisted of three different concentrations of rotten guavas, such as 20 (T1), 40 (T2) and 60 (T3) were allowed to digest under aeration. After 34 days, three replications showed the reddish white-colored supernatant screened and taken in a 2.0 L conical flask. Then, Spirulina was inoculated to grow in these three digested rotten guava media (DRGM) with the addition of micronutrients and KM and 9.0 g/L NaHCO3 for 14 days. The cell weight of Spirulina was attained at a maximum of 12.43±0.10 mg/L (dry wt. basis) in KM, followed by 0.818 ± 0.002, 0.815 ± 0.0014 and 0.809± 0.0011 mg/L in supernatant of 60, 20 and 40% DRGM, respectively on the 10th day of culture. A similar trend was also observed in the case of specific growth rates (based on cell weight and chlorophyll a) and total biomass (mg/L) of Spirulina. Cell weight of Spirulina had a highly significant (P<0.01) correlation with the chlorophyll a content (r= 0.746) and total biomass (r= 0.742) of Spirulina. The results on the growth performance showed that Spirulina grown in supernatant of 60% DRGM was significantly (P<0.01) higher (T3) than that of Spirulina grown in supernatant of 20% (T1) and 40% DRGM (T2).
Key Words: Culture potentiality, Growth performance, Spirulina platensis, Digested Rotten Guava Media (DRGM)
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Article Citations:
MLA
Islam, M. S. et al. “The growth performance of Spirulina Platensis in supernatant of digested rotten guava”. Journal of Bioscience and Agriculture Research, 29(01), (2022): 2433-2441.
APA
Islam, M. S., Habib, M. A. B., Rahman, M. H. and Arifuzzaman, M. (2022). The growth performance of Spirulina Platensis in supernatant of digested rotten guava. Journal of Bioscience and Agriculture Research, 29(01), 2433-2441.
Chicago
Islam, M. S., Habib, M. A. B., Rahman, M. H. and Arifuzzaman, M. “The growth performance of Spirulina Platensis in supernatant of digested rotten guava”. Journal of Bioscience and Agriculture Research, 29(01), (2022): 2433-2441.
Harvard
Islam, M. S., Habib, M. A. B., Rahman, M. H. and Arifuzzaman, M. 2022. The growth performance of Spirulina Platensis in supernatant of digested rotten guava. Journal of Bioscience and Agriculture Research, 29(01), pp. 2433-2441.
Vancouver
Islam, MS, Habib, MAB, Rahman, MH and Arifuzzaman, M. The growth performance of Spirulina Platensis in supernatant of digested rotten guava. Journal of Bioscience and Agriculture Research, 2022 August, 29(01): 2433-2441.
Islam, M. S. et al. “The growth performance of Spirulina Platensis in supernatant of digested rotten guava”. Journal of Bioscience and Agriculture Research, 29(01), (2022): 2433-2441.
APA
Islam, M. S., Habib, M. A. B., Rahman, M. H. and Arifuzzaman, M. (2022). The growth performance of Spirulina Platensis in supernatant of digested rotten guava. Journal of Bioscience and Agriculture Research, 29(01), 2433-2441.
Chicago
Islam, M. S., Habib, M. A. B., Rahman, M. H. and Arifuzzaman, M. “The growth performance of Spirulina Platensis in supernatant of digested rotten guava”. Journal of Bioscience and Agriculture Research, 29(01), (2022): 2433-2441.
Harvard
Islam, M. S., Habib, M. A. B., Rahman, M. H. and Arifuzzaman, M. 2022. The growth performance of Spirulina Platensis in supernatant of digested rotten guava. Journal of Bioscience and Agriculture Research, 29(01), pp. 2433-2441.
Vancouver
Islam, MS, Habib, MAB, Rahman, MH and Arifuzzaman, M. The growth performance of Spirulina Platensis in supernatant of digested rotten guava. Journal of Bioscience and Agriculture Research, 2022 August, 29(01): 2433-2441.
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