J. Biosci. Agric. Res. | Volume 29, Issue 02, 2472-2483 2| https://doi.org/10.18801/jbar.290222.299
Article type: Research article | Received: 06.06.2022; Revised: 15.11.2022; First published online: 29 December, 2022.
Article type: Research article | Received: 06.06.2022; Revised: 15.11.2022; First published online: 29 December, 2022.
Isolation of pathogenic Streptomyces spp. and determination of variability among the strains collected from potato
Ashis Kumar Saha 1, Najmun Naher 2, Md. Eakramul Haque 3, Md. Harun-Or-Rashid 1 and Md. Muzahid-E-Rahman 1
1 Regional Agricultural Research Station, Bangladesh Agricultural Research Institute, Burirhat, Rangpur, Bangladesh
2 Department of Botany, National University, Gazipur, Bangladesh
3 On Farm Research Division, Bangladesh Agricultural Research Institute, Rangpur, Bangladesh.
✉ Corresponding author: [email protected] (Saha AK).
1 Regional Agricultural Research Station, Bangladesh Agricultural Research Institute, Burirhat, Rangpur, Bangladesh
2 Department of Botany, National University, Gazipur, Bangladesh
3 On Farm Research Division, Bangladesh Agricultural Research Institute, Rangpur, Bangladesh.
✉ Corresponding author: [email protected] (Saha AK).
Abstract
A study was undertaken during 2008-2009, 2009-2010 and 2010-11 cropping seasons to isolate the variability(s) of Streptomyces spp. from lesion of common scab affected potato tubers in the Northern parts of Bangladesh (Rangpur division). There were twelve different pathogenic species isolated, which were different for colony colour, spore colour, spore ornamentation, spore chain morphology, other physiological characteristics (temperature, pH, melanin production, colour secret in oatmeal broth medium, carbon utilization and growth response in NaCl solution) and pathogenic reaction. Overall all collected isolates grow well within temperature range of 25°C to 30°C. In the present investigation, it was observed that isolates S2 and S11 could grow at pH 4.50. It was found that the growth of isolates increased as the pH value of medium increased. Considering all the characteristics of different isolates, isolate S5 agreed with the description of S. scabies having smooth, grey spores borne in spiral chains, producing melanin and utilizing eight recommended ISP diagnostic sugars with raffinose as a sole source of carbon. Rest eleven isolates could not identify.
Key words: Common scab, pH, OMB medium, Melanin and Pathogenicity
A study was undertaken during 2008-2009, 2009-2010 and 2010-11 cropping seasons to isolate the variability(s) of Streptomyces spp. from lesion of common scab affected potato tubers in the Northern parts of Bangladesh (Rangpur division). There were twelve different pathogenic species isolated, which were different for colony colour, spore colour, spore ornamentation, spore chain morphology, other physiological characteristics (temperature, pH, melanin production, colour secret in oatmeal broth medium, carbon utilization and growth response in NaCl solution) and pathogenic reaction. Overall all collected isolates grow well within temperature range of 25°C to 30°C. In the present investigation, it was observed that isolates S2 and S11 could grow at pH 4.50. It was found that the growth of isolates increased as the pH value of medium increased. Considering all the characteristics of different isolates, isolate S5 agreed with the description of S. scabies having smooth, grey spores borne in spiral chains, producing melanin and utilizing eight recommended ISP diagnostic sugars with raffinose as a sole source of carbon. Rest eleven isolates could not identify.
Key words: Common scab, pH, OMB medium, Melanin and Pathogenicity
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Article Citations:
MLA
Saha, A. K. et al. “Isolation of pathogenic Streptomyces spp. and determination of variability among the strains collected from potato”. Journal of Bioscience and Agriculture Research, 29(02) (2022): 2472-2483.
APA
Saha, A. K., Naher, N., Haque, M. E., Rashid, M. H. and Rahman, M. M. E. (2022). Isolation of pathogenic Streptomyces spp. and determination of variability among the strains collected from potato. Journal of Bioscience and Agriculture Research, 29(02), 2472-2483.
Chicago
Saha, A. K., Naher, N., Haque, M. E., Rashid, M. H. and Rahman, M. M. E. “Isolation of pathogenic Streptomyces spp. and determination of variability among the strains collected from potato”. Journal of Bioscience and Agriculture Research, 29(02) (2022): 2472-2483.
Harvard
Saha, A. K., Naher, N., Haque, M. E., Rashid, M. H. and Rahman, M. M. E. 2022. Isolation of pathogenic Streptomyces spp. and determination of variability among the strains collected from potato. Journal of Bioscience and Agriculture Research, 29(02), pp. 2472-2483.
Vancouver
Saha, AK, Naher, N, Haque, ME, Rashid, MH and Rahman, MME. Isolation of pathogenic Streptomyces spp. and determination of variability among the strains collected from potato. Journal of Bioscience and Agriculture Research, 2022 December 29(02), 2472-2483.
Saha, A. K. et al. “Isolation of pathogenic Streptomyces spp. and determination of variability among the strains collected from potato”. Journal of Bioscience and Agriculture Research, 29(02) (2022): 2472-2483.
APA
Saha, A. K., Naher, N., Haque, M. E., Rashid, M. H. and Rahman, M. M. E. (2022). Isolation of pathogenic Streptomyces spp. and determination of variability among the strains collected from potato. Journal of Bioscience and Agriculture Research, 29(02), 2472-2483.
Chicago
Saha, A. K., Naher, N., Haque, M. E., Rashid, M. H. and Rahman, M. M. E. “Isolation of pathogenic Streptomyces spp. and determination of variability among the strains collected from potato”. Journal of Bioscience and Agriculture Research, 29(02) (2022): 2472-2483.
Harvard
Saha, A. K., Naher, N., Haque, M. E., Rashid, M. H. and Rahman, M. M. E. 2022. Isolation of pathogenic Streptomyces spp. and determination of variability among the strains collected from potato. Journal of Bioscience and Agriculture Research, 29(02), pp. 2472-2483.
Vancouver
Saha, AK, Naher, N, Haque, ME, Rashid, MH and Rahman, MME. Isolation of pathogenic Streptomyces spp. and determination of variability among the strains collected from potato. Journal of Bioscience and Agriculture Research, 2022 December 29(02), 2472-2483.
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