Journal of Fisheries, Livestock and Veterinary Science
You are here: Home>JFLVS Journal>JBAR Archive>Article Page: jflvs-020121-07.html
J. Fish. Livest. Vet. Res. | Volume 02, Issue 01, 57-67 | https://doi.org/10.18801/jflvs.020121.07
Article type: Research article | Received: 10.11.21; Revised: 21.12.21; First published online: 29 December 2021.
Article type: Research article | Received: 10.11.21; Revised: 21.12.21; First published online: 29 December 2021.
A better and copacetic protocol for histopathological slide preparation using H&E stain: A review
Md. Iftakharul Hasan 1, Shonkor Kumar Das 1 and Emdadul Haque Chowdhury 2
1 Dept. of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
2 Dept. of Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
✉ Corresponding author: hrafi720@gmail.com (Hasan, MI).
1 Dept. of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
2 Dept. of Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
✉ Corresponding author: hrafi720@gmail.com (Hasan, MI).
Abstract
The hematoxylin and eosin (H&E) stain is one of the most common stains which is frequently used all over the world for histochemical and histopathological results or observations. Many hematoxylin and eosin (H&E) stain protocols are used in the laboratories, but sometimes after following the protocol, the slides do not produce a better visualization, which affects the results massively. H&E staining faults can result from any problems in the key steps fixation, processing, embedding, sectioning, and staining. Also, inaccurate timing of staining, not considering the quality and stability of the stain, not following the protocol can cause damage to the slide and ultimately the result. A respectable number of papers, articles and books are reviewed and several practical protocols are analyzed; therefore, this study is done at the Department of Anatomy and Histology & Department of Pathology in Bangladesh Agricultural University, Mymensingh to determine and produce a better copacetic protocol for H&E stain for precise and actual visualization of slide when viewing in light microscope; which will help to render a better slide and produce an accurate result every time.
Key Words: Histopathological slide, H&E, Stain, Histopathology and Tissue visualization protocol
The hematoxylin and eosin (H&E) stain is one of the most common stains which is frequently used all over the world for histochemical and histopathological results or observations. Many hematoxylin and eosin (H&E) stain protocols are used in the laboratories, but sometimes after following the protocol, the slides do not produce a better visualization, which affects the results massively. H&E staining faults can result from any problems in the key steps fixation, processing, embedding, sectioning, and staining. Also, inaccurate timing of staining, not considering the quality and stability of the stain, not following the protocol can cause damage to the slide and ultimately the result. A respectable number of papers, articles and books are reviewed and several practical protocols are analyzed; therefore, this study is done at the Department of Anatomy and Histology & Department of Pathology in Bangladesh Agricultural University, Mymensingh to determine and produce a better copacetic protocol for H&E stain for precise and actual visualization of slide when viewing in light microscope; which will help to render a better slide and produce an accurate result every time.
Key Words: Histopathological slide, H&E, Stain, Histopathology and Tissue visualization protocol
Article Full-Text PDF:
| Click here to upload file |
|
Share This Article:
|
|
Article Citations:
MLA
Hasan, M. I. “A better and copacetic protocol for histopathological slide preparation using H&E stain: A review”. Journal of Fisheries, Livestock and Veterinary Science 02(02) (2021): 57-67.
APA
Hasan, M. I., Das, S. K. and Chowdhury, E. H. (2021). A better and copacetic protocol for histopathological slide preparation using H&E stain: A review. Journal of Fisheries, Livestock and Veterinary Science, 02(02), 57-67.
Chicago
Hasan, M. I., Das, S. K. and Chowdhury, E. H. A better and copacetic protocol for histopathological slide preparation using H&E stain: A review”. Journal of Fisheries, Livestock and Veterinary Science. 02(02) (2021): 57-67.
Harvard
Hasan, M. I., Das, S. K. and Chowdhury, E. H. 2021. A better and copacetic protocol for histopathological slide preparation using H&E stain: A review. Journal of Fisheries, Livestock and Veterinary Science, 02(02), pp. 57-67.
Vancouver
Hasan MI, Das, SK and Chowdhury, EH. A better and copacetic protocol for histopathological slide preparation using H&E stain: A review. Journal of Fisheries, Livestock and Veterinary Science. 2021 December 02(02):57-67.
Hasan, M. I. “A better and copacetic protocol for histopathological slide preparation using H&E stain: A review”. Journal of Fisheries, Livestock and Veterinary Science 02(02) (2021): 57-67.
APA
Hasan, M. I., Das, S. K. and Chowdhury, E. H. (2021). A better and copacetic protocol for histopathological slide preparation using H&E stain: A review. Journal of Fisheries, Livestock and Veterinary Science, 02(02), 57-67.
Chicago
Hasan, M. I., Das, S. K. and Chowdhury, E. H. A better and copacetic protocol for histopathological slide preparation using H&E stain: A review”. Journal of Fisheries, Livestock and Veterinary Science. 02(02) (2021): 57-67.
Harvard
Hasan, M. I., Das, S. K. and Chowdhury, E. H. 2021. A better and copacetic protocol for histopathological slide preparation using H&E stain: A review. Journal of Fisheries, Livestock and Veterinary Science, 02(02), pp. 57-67.
Vancouver
Hasan MI, Das, SK and Chowdhury, EH. A better and copacetic protocol for histopathological slide preparation using H&E stain: A review. Journal of Fisheries, Livestock and Veterinary Science. 2021 December 02(02):57-67.
References:
- Anderson, J. (2011). An introduction to Routine and special staining. http://www.leicabiosystems.com/pathologyleaders/an-introduction-to-routine-and-special-staining.
- Andrew, H. F., Kenneth, A. J., Jack, R. and Rolf, Z (2008). Hematoxylin and Eosin Staining of Tissue and cell sections. Cold spring harb protocols, 2008(5), http://dx.doi.org/10.1101/pdb.prot4986 .
- Avwioro, G. (2011). Histochemical Uses of Haematoxylin - A Review. Journal of Physics: Conference Series. 1(5), 24-34.
https://www.arpapress.com/Volumes/JPCS/Vol1/JPCS_1_05.pdf - Bancroft, J. D. and Layton, C. (2013). The Hematoxylin and eosin. In S. K. Suvarna, C. Layton, & J. D. Bancroft (Eds.), Theory & Practice of histological techniques (7th ed., Ch. 10 and 11, pp. 179-220). Philadelphia: Churchill Livingstone of El Sevier. http://dx.doi.org/10.1016/b978-0-7020-4226-3.00010-x
- Black, J. (2012). Microbiology: Principles and exploration. 8th ed. John Wiley Sons; p. 68.
- Cai, H., Caswell, J. L., and Prescott, J. F. (2014). Nonculture Molecular Techniques for Diagnosis of Bacterial Disease in Animals: A Diagnostic Laboratory Perspective. Veterinary Pathology, 51(2), 341-350. http://dx.doi.org/10.1177/0300985813511132
- Chan, J. K. C. (2014). The wonderful colors of the hematoxylin-eosin stain in diagnostic surgical pathology. International Journal of Surgical Pathology, 22(1), 12–32.
- Charley, H. (1982). Food science, 2nd edn. Macmillan Publishing Company, New York.
- Christina, E. Day. (Eds). (2014). Histopathology- Methods and Protocols. Humana Press New York, USA.
- Cindy, S. and Geoffrey, R. (2021). H&E Staining Overview: A Guide to Best Practices. Richmond, Leica Biosystems. https://www.leicabiosystems.com/knowledge-pathway/he-staining-overview-a-guide-to-best-practices/
- Costa, C. A. X., Brito, K. N. O., Gomes, M. A. and Caliari, M. V. (2010). Histopathological and immunohistochemical study of the hepatic lesions experimentally induced by Entamoeba. European Journal of Histochemistry, 54(3), e39. http://dx.doi.org/10.4081/ejh.2010.e39
- Culling, C. F. A. (1984). Handbook of Histopathological and Histochemical Techniques -3rd Edition, Butterworth & Co. (Publishers) Ltd, 1974.
- Hegazy, R. and Hegazy, A. (2015). Hegazy’ Simplified Method of Tissue Processing (Consuming Time and Chemicals). Annals of International Medical and Dental Research, 1(2), 57-61.
- Iyiola, S. and Avwioro, O. G. (2011). Alum haematoxylin stain for the demonstration of nuclear and extra nuclear substances. Journal of Pharmacy and Clinical Sciences, 1, 4.
- Jackson, P. and Blythe, D. (2013). Theory Practice of histological techniques. In Suvarna, S. K. Layton, C. and Bancroft, J. D. 7th Edition of Bancroft's Theory and Practice of Histological Techniques. Churchill Livingstone, pp. 386–431. http://dx.doi.org/10.1016/b978-0-7020-4226-3.00018-4
- Larson, K, Ho, H. H. and Anumolu, P.L., et al. (2011). Hematoxylin and eosin tissue stain in Mohs micrographic surgery: a review. Dermatologic Surgery, 37(8), 10891099.
- McGavin, M. D. (2014). Factors affecting visibility of a target tissue in histologic sections. Veterinary Pathology, 51(1), 9-27. http://dx.doi.org/10.1177/0300985813506916
- Musumeci, G. (2014). Past, present and future: overview on Histology and histopathology. Journal of Histology & Histopathology, 1, 5. http://dx.doi.org/10.7243/2055-091X-1-5
- Ortiz-Hidalgo, C. and Pina-Oviedo, S. (2019). Hematoxylin: Mesoamerica’s gift to histopathology. Palo de Campeche (logwood tree), pirates’ most desired treasure, and irreplaceable tissue stain. International Journal of Surgical Pathology, 27(1), 4–14.
- Robert, D. Cardiff, Claramae, H. Miller and Robert J. Munn. (2014). Manual Hematoxylin and Eosin Staining of Mouse Tissue Sections -Cold spring harbor protocols; http://dx.doi.org/ 10.1101/pdb.prot073411.
- Rodrigues, E. B., Costa, E. F., Penha, F. M., Melo, G. B., Bottós, J., Dib, E. and Farah, M. E. (2009). The use of vital dyes in ocular surgery. Survey of Ophthalmology, 54(5), 576-617. http://dx.doi.org/10.1016/j.survophthal.2009.04.011
- Rolls, G. (2018). An Introduction to Specimen Preparation. Leica Biosystems. https://www.leicabiosystems.com/knowledge-pathway/an-introduction-to-specimen-preparation.
- Shostak, S. (2013). Histology Nomenclature: Past, Present and Future. Biological Systems: Open Access 2.122 (2013).
- Silverman, D. (Eds). (2011). Qualitative Research: Issues of Theory, Method and Practice (3rd ed.). London, Thousand Oaks, New Delhi, Singapore: Sage Publications.
- Titford, M. (2009). Progress in the development of microscopical techniques for diagnostic pathology. Journal of Histotechnology. 32:9–19. http://dx.doi.org/10.1179/his.2009.32.1.9 .
- Titford, M. and Bowman, B. (2012). What May the Future Hold for Histotechnologists? Laboratory Medicine, 43(suppl_2), e5-e10.
- Weiss, A. T., Delcour, N. M., Meyer, A. and Klopfleisch, R. (2010). Efficient & Cost-Effective Extraction of Genomic DNA from Formalin-Fixed and Paraffin-Embedded Tissues. Veterinary Pathology, 22(4), 834-838.
- Young, B., O'Dowd, G. and Stewart, W. (2010). Wheater's Basic Pathology: A Text, Atlas and Review of Histopathology (5th Ed.). Churchill Livingstone.
- Zhou, X. and Moore. B. B. (2017). Lung Section Staining and Microscopy. Bio-Protocol, 7(10), e2286. http://dx.doi.org/10.21769/BioProtoc.2286.
© 2021 The Authors. This article is freely available for anyone to read, share, download, print, permitted for unrestricted use and build upon, provided that the original author(s) and publisher are given due credit. All Published articles are distributed under the Creative Commons Attribution 4.0 International License.
Journal of Fisheries, Livestock and Veterinary Science.