Journal of Fisheries, Livestock and Veterinary Science
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J. Fish. Livest. Vet. Res. | Volume 02, Issue 01, 57-67 | https://doi.org/10.18801/jflvs.020121.07
Article type: Research article | Received: 10.11.21; Revised: 21.12.21; First published online: 29 December 2021.
Article type: Research article | Received: 10.11.21; Revised: 21.12.21; First published online: 29 December 2021.
A better and copacetic protocol for histopathological slide preparation using H&E stain: A review
Md. Iftakharul Hasan 1, Shonkor Kumar Das 1 and Emdadul Haque Chowdhury 2
1 Dept. of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
2 Dept. of Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
✉ Corresponding author: [email protected] (Hasan, MI).
1 Dept. of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
2 Dept. of Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
✉ Corresponding author: [email protected] (Hasan, MI).
Abstract
The hematoxylin and eosin (H&E) stain is one of the most common stains which is frequently used all over the world for histochemical and histopathological results or observations. Many hematoxylin and eosin (H&E) stain protocols are used in the laboratories, but sometimes after following the protocol, the slides do not produce a better visualization, which affects the results massively. H&E staining faults can result from any problems in the key steps fixation, processing, embedding, sectioning, and staining. Also, inaccurate timing of staining, not considering the quality and stability of the stain, not following the protocol can cause damage to the slide and ultimately the result. A respectable number of papers, articles and books are reviewed and several practical protocols are analyzed; therefore, this study is done at the Department of Anatomy and Histology & Department of Pathology in Bangladesh Agricultural University, Mymensingh to determine and produce a better copacetic protocol for H&E stain for precise and actual visualization of slide when viewing in light microscope; which will help to render a better slide and produce an accurate result every time.
Key Words: Histopathological slide, H&E, Stain, Histopathology and Tissue visualization protocol
The hematoxylin and eosin (H&E) stain is one of the most common stains which is frequently used all over the world for histochemical and histopathological results or observations. Many hematoxylin and eosin (H&E) stain protocols are used in the laboratories, but sometimes after following the protocol, the slides do not produce a better visualization, which affects the results massively. H&E staining faults can result from any problems in the key steps fixation, processing, embedding, sectioning, and staining. Also, inaccurate timing of staining, not considering the quality and stability of the stain, not following the protocol can cause damage to the slide and ultimately the result. A respectable number of papers, articles and books are reviewed and several practical protocols are analyzed; therefore, this study is done at the Department of Anatomy and Histology & Department of Pathology in Bangladesh Agricultural University, Mymensingh to determine and produce a better copacetic protocol for H&E stain for precise and actual visualization of slide when viewing in light microscope; which will help to render a better slide and produce an accurate result every time.
Key Words: Histopathological slide, H&E, Stain, Histopathology and Tissue visualization protocol
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Article Citations:
MLA
Hasan, M. I. “A better and copacetic protocol for histopathological slide preparation using H&E stain: A review”. Journal of Fisheries, Livestock and Veterinary Science 02(02) (2021): 57-67.
APA
Hasan, M. I., Das, S. K. and Chowdhury, E. H. (2021). A better and copacetic protocol for histopathological slide preparation using H&E stain: A review. Journal of Fisheries, Livestock and Veterinary Science, 02(02), 57-67.
Chicago
Hasan, M. I., Das, S. K. and Chowdhury, E. H. A better and copacetic protocol for histopathological slide preparation using H&E stain: A review”. Journal of Fisheries, Livestock and Veterinary Science. 02(02) (2021): 57-67.
Harvard
Hasan, M. I., Das, S. K. and Chowdhury, E. H. 2021. A better and copacetic protocol for histopathological slide preparation using H&E stain: A review. Journal of Fisheries, Livestock and Veterinary Science, 02(02), pp. 57-67.
Vancouver
Hasan MI, Das, SK and Chowdhury, EH. A better and copacetic protocol for histopathological slide preparation using H&E stain: A review. Journal of Fisheries, Livestock and Veterinary Science. 2021 December 02(02):57-67.
Hasan, M. I. “A better and copacetic protocol for histopathological slide preparation using H&E stain: A review”. Journal of Fisheries, Livestock and Veterinary Science 02(02) (2021): 57-67.
APA
Hasan, M. I., Das, S. K. and Chowdhury, E. H. (2021). A better and copacetic protocol for histopathological slide preparation using H&E stain: A review. Journal of Fisheries, Livestock and Veterinary Science, 02(02), 57-67.
Chicago
Hasan, M. I., Das, S. K. and Chowdhury, E. H. A better and copacetic protocol for histopathological slide preparation using H&E stain: A review”. Journal of Fisheries, Livestock and Veterinary Science. 02(02) (2021): 57-67.
Harvard
Hasan, M. I., Das, S. K. and Chowdhury, E. H. 2021. A better and copacetic protocol for histopathological slide preparation using H&E stain: A review. Journal of Fisheries, Livestock and Veterinary Science, 02(02), pp. 57-67.
Vancouver
Hasan MI, Das, SK and Chowdhury, EH. A better and copacetic protocol for histopathological slide preparation using H&E stain: A review. Journal of Fisheries, Livestock and Veterinary Science. 2021 December 02(02):57-67.
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