J. Mol. Stud. Med. Res. | Volume 01, Issue 01, 01-15| https://doi.org/10.18801/jmsmr.010115.01
Article type: Research article.
Article type: Research article.
Quantifying the role of arbuscular mycorrhizal colonization and acid phosphatase activity in grass biomass production
Sultana, J. (1) and Siddique, M. N. A. (2)
1Soil Quality Group, Soil Biology and Biological Soil Quality Organic Agriculture, Wageningen University, the Netherlands.
2Soil Resource Development Institute, Ministry of Agriculture, Bangladesh.
1Soil Quality Group, Soil Biology and Biological Soil Quality Organic Agriculture, Wageningen University, the Netherlands.
2Soil Resource Development Institute, Ministry of Agriculture, Bangladesh.
Abstract
Phosphorus (P) is one of the most important plant nutrients but a large portion of soil phosphorus (P) is not available to plants due to sequestration in organic forms. Phosphatase enzymes play an important role in the process, whereby the phosphate molecule is split off from the organic compound and these enzymes are originated from plant roots, mycorrhizal fungi and rhizosphere fungi and bacteria. It is still unclear to what extent these enzymes and arbuscular mycorrhizal symbiosis affect plant biomass. The aim of study is to quantify the role of acid phosphatase activity (APA) and arbuscular mycorrhizal colonization (AMF) in plant biomass and their correlation. A pot experiment of eight different grass species Lolium perenne-cv. Diploid, Lolium perenne-cv. Tetraploid, Lolium multiflorum, Festuca arundinacea, Poa trivialis, Poa pratensis, Phleum pratense, Holcus lanatus grown on a strongly P-fixing soil with two P treatments (with P fertilizer and without P fertilizer) was established. Rhizosphere soil for phosphatase activity, pH and roots for mycorrhizal colonization were collected. AMF colonization significantly differs with P treatments and species had no significant effect, although P treatments and species had no significant effect on acid phosphatase activity and pH. On the other hand, both had highly significant effect on dry weight but the interaction effect between P treatment and species also decline over time. A positive correlation between APA and dry weight in P deficient condition while APA and dry weight positively correlated to pH at fertilization with P. P deficient conditions encourage AMF colonization. The lack of significance differences between species suggests that higher variability of APA may be due to higher pH. Due to same back ground and nutritional status, species and P treatments not any impact on pH although P fertilization had a significant effect on dry weight indicating that plants require P for optimum growth. Species had positive impact on dry weight due to different resource use efficiency. P fertilization effect declines over time due to highly P-fixing capacity of soil. The variation of APA occurs due to pH changes, this positive correlation between dry weight and pH effect can be linked to variability of nutrient content.
Key words: APA, AMF colonization, dry weight, pH, phosphorus and grass
Phosphorus (P) is one of the most important plant nutrients but a large portion of soil phosphorus (P) is not available to plants due to sequestration in organic forms. Phosphatase enzymes play an important role in the process, whereby the phosphate molecule is split off from the organic compound and these enzymes are originated from plant roots, mycorrhizal fungi and rhizosphere fungi and bacteria. It is still unclear to what extent these enzymes and arbuscular mycorrhizal symbiosis affect plant biomass. The aim of study is to quantify the role of acid phosphatase activity (APA) and arbuscular mycorrhizal colonization (AMF) in plant biomass and their correlation. A pot experiment of eight different grass species Lolium perenne-cv. Diploid, Lolium perenne-cv. Tetraploid, Lolium multiflorum, Festuca arundinacea, Poa trivialis, Poa pratensis, Phleum pratense, Holcus lanatus grown on a strongly P-fixing soil with two P treatments (with P fertilizer and without P fertilizer) was established. Rhizosphere soil for phosphatase activity, pH and roots for mycorrhizal colonization were collected. AMF colonization significantly differs with P treatments and species had no significant effect, although P treatments and species had no significant effect on acid phosphatase activity and pH. On the other hand, both had highly significant effect on dry weight but the interaction effect between P treatment and species also decline over time. A positive correlation between APA and dry weight in P deficient condition while APA and dry weight positively correlated to pH at fertilization with P. P deficient conditions encourage AMF colonization. The lack of significance differences between species suggests that higher variability of APA may be due to higher pH. Due to same back ground and nutritional status, species and P treatments not any impact on pH although P fertilization had a significant effect on dry weight indicating that plants require P for optimum growth. Species had positive impact on dry weight due to different resource use efficiency. P fertilization effect declines over time due to highly P-fixing capacity of soil. The variation of APA occurs due to pH changes, this positive correlation between dry weight and pH effect can be linked to variability of nutrient content.
Key words: APA, AMF colonization, dry weight, pH, phosphorus and grass
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MLA
Sultana and Siddique. “Quantifying the role of arbuscular mycorrhizal colonization and acid phosphatase activity in grass biomass production.” Journal of Molecular Studies and Medicine Research 01(01) (2015): 01-15.
APA
Sultana, J. and Siddique, M. N. A. (2015). Quantifying the role of arbuscular mycorrhizal colonization and acid phosphatase activity in grass biomass production. Journal of Molecular Studies and Medicine Research, 01(01), 01-15.
Chicago
Sultana, J. and Siddique, M. N. A. “Quantifying the role of arbuscular mycorrhizal colonization and acid phosphatase activity in grass biomass production.” Journal of Molecular Studies and Medicine Research 01(01) (2015): 01-15.
Harvard
Sultana, J. and Siddique, M. N. A. 2015. Quantifying the role of arbuscular mycorrhizal colonization and acid phosphatase activity in grass biomass production. Journal of Molecular Studies and Medicine Research, 01(01), pp. 01-15.
Vancouver
Sultana, J. and Siddique, M. N. A. Quantifying the role of arbuscular mycorrhizal colonization and acid phosphatase activity in grass biomass production. Journal of Molecular Studies and Medicine Research. 2015, 01(01): 01-15.
Sultana and Siddique. “Quantifying the role of arbuscular mycorrhizal colonization and acid phosphatase activity in grass biomass production.” Journal of Molecular Studies and Medicine Research 01(01) (2015): 01-15.
APA
Sultana, J. and Siddique, M. N. A. (2015). Quantifying the role of arbuscular mycorrhizal colonization and acid phosphatase activity in grass biomass production. Journal of Molecular Studies and Medicine Research, 01(01), 01-15.
Chicago
Sultana, J. and Siddique, M. N. A. “Quantifying the role of arbuscular mycorrhizal colonization and acid phosphatase activity in grass biomass production.” Journal of Molecular Studies and Medicine Research 01(01) (2015): 01-15.
Harvard
Sultana, J. and Siddique, M. N. A. 2015. Quantifying the role of arbuscular mycorrhizal colonization and acid phosphatase activity in grass biomass production. Journal of Molecular Studies and Medicine Research, 01(01), pp. 01-15.
Vancouver
Sultana, J. and Siddique, M. N. A. Quantifying the role of arbuscular mycorrhizal colonization and acid phosphatase activity in grass biomass production. Journal of Molecular Studies and Medicine Research. 2015, 01(01): 01-15.
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