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Journal of Molecular Studies and Medicine Research 
Research article: 
Micronutrient evaluation of fortified soymilk from sprouted whole soybean for complementary feeding using response surface methodology
 
I. N. Okwunodulu and M. O. Iwe
 
Dept. of Food Science and Technology, Michael Okpara University of Agriculture, Umudike, Abia State Nigeria

J. mole. stud. medicin. res, volume 01, issue 01, pp. 16-25 | Available online: 24 December 2015
DOI: http://dx.doi.org/10.18801/jmsmr.010115.02
micronutrient_evaluation_of_fortified_soymilk_from_sprouted_whole_soybean_for_complementary_feeding_using_response_surface_methodology.pdf
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Abstract: Soymilk from TGX 923-2E soybean variety sprouted for 72hrs after 12 hours tap steeping was fortified with ferric ammonium citrate (Fe), calcium (Ca) and vitamin C (VC) according to Box-Wilson (1951) experimental design matrix. A central composite rotatable response surface design(CCRRSD) for K = 3 was employed to examine the linear, quadratic and cross product effects of independent process variable concentrations at 5 levels, Fe (1, 2, 3, 4 and 5mg/100ml), Ca (50, 100, 150, 200, and 250mg/100ml) and VC (8, 16, 24, 32 and 40mg/100ml) on the nutrient density of fortified soymilk stored at ambient temperature for 12 weeks. A total of 21 experimental runs were generated and the response surface data for each run were statistically regressed and analyzed for variance using Minitab computer software (version 11.21). Three-dimensional response surface figures were plotted with Matlab software (version R 2007b) to visualize the effects of fortificants variables on the responses. Results obtained showed that all the fortificants were significantly (p ≤ 0.05) linearly related to their respective responses. None of the response micronutrient met the respective RDI per serving except samples with variable combinations 2, 100, 32; 2, 200, 32; 4, 200, 32 and 3, 150, 40mg/100ml for ferric ammonium citrate, calcium carbonate and vitamin C respectively.
Key words: Complementary feeding, micronutrient, sprouted soybean, fortified soymilk and response surface methodology
APA (American Psychological Association)
Okwunodulu, I. N. & Iwe, M. O. (2015). Micronutrient evaluation of fortified soymilk from sprouted whole soybean for complementary feeding using response surface methodology. Journal of Molecular Studies and Medicine Research, 01(01), 16-25.
 
MLA (Modern Language Association) 
Okwunodulu, I. N. & Iwe, M. O.  “Micronutrient evaluation of fortified soymilk from sprouted whole soybean for complementary feeding using response surface methodology.” Journal of Molecular Studies and Medicine Research, 01.01 (2015): 16-251.
​ 
Chicago/Turabian
Okwunodulu, I. N. & Iwe, M. O.  “Micronutrient evaluation of fortified soymilk from sprouted whole soybean for complementary feeding using response surface methodology.” Journal of Molecular Studies and Medicine Research, 01, no. 01 (2015): 16-25.
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