RESEARCH ARTICLE:
OXA-181- an emerging threat in Bangladesh
Sarmin Satter (1*), Hasan Mahbub (2) and S. M. Shamsuzzaman (3)
1Dept. of Microbiology, CARE Medical College, Dhaka
2Dept. of Neurosurgery, Dhaka Medical College Hospital, Dhaka
3Dept. of Microbiology, Dhaka Medical College, Dhaka, Bangladesh
ARTICLE INFORMATION:
J. Mole. Stud. Medici. Res. Volume 03, Issue 01, pp. 128-134| First available online: 14 October 2018
Crossref: https://doi.org/10.18801/jmsmr.030118.15
For any query email to [email protected]
OXA-181- an emerging threat in Bangladesh
Sarmin Satter (1*), Hasan Mahbub (2) and S. M. Shamsuzzaman (3)
1Dept. of Microbiology, CARE Medical College, Dhaka
2Dept. of Neurosurgery, Dhaka Medical College Hospital, Dhaka
3Dept. of Microbiology, Dhaka Medical College, Dhaka, Bangladesh
ARTICLE INFORMATION:
J. Mole. Stud. Medici. Res. Volume 03, Issue 01, pp. 128-134| First available online: 14 October 2018
Crossref: https://doi.org/10.18801/jmsmr.030118.15
For any query email to [email protected]
ARTICLE FULL TEXT:
| 15.03.01.18_oxa_181_an_emerging_threat_in_bangladesh.pdf |
Title: OXA-181- an emerging threat in Bangladesh
Abstract
This study was conducted to detect the prevalence of OXA-181 gene producing Escherichia coli and Klebsiella spp. in a tertiary care hospital of Bangladesh. Total 166 Escherichia coli and Klebsiella spp. were isolated from urine, wound swab, pus, blood and sputum samples in Dhaka Medical College Hospital. Antibiotic susceptibility test was performed by disk-diffusion technique. Carbapenemase producers were detected phenotypically by Modified Hodge test. Carbapenemase genes (blaNDM-1, blaVIM, blaIMP, blaKPC, blaOXA-48/blaOXA-181) among imipenem resistant strains among the isolated Escherichia coli and Klebsiella spp. were detected by PCR. Sequencing was done to differentiate blaOXA-181 from blaOXA-48. Thirty-seven (22.29%) imipenem resistant isolates were detected during disk-diffusion technique, among them 43.24% carbapenemase producers were detected by MHT and 62.16% by PCR. NDM-1 (43.24%) was the dominant genotype followed by KPC (21.62%) and OXA-181 (18.92%). The results of this study showed presence of high proportion of carbapenemase enzyme producing Escherichia coli and Klebsiella spp. in Bangladesh. BlaOXA-181 gene is emerging in Bangladesh.
Key Words: Escherichia coli, Klebsiella spp., Carbapenemase, BlaOXA-181 gene and Bangladesh
Abstract
This study was conducted to detect the prevalence of OXA-181 gene producing Escherichia coli and Klebsiella spp. in a tertiary care hospital of Bangladesh. Total 166 Escherichia coli and Klebsiella spp. were isolated from urine, wound swab, pus, blood and sputum samples in Dhaka Medical College Hospital. Antibiotic susceptibility test was performed by disk-diffusion technique. Carbapenemase producers were detected phenotypically by Modified Hodge test. Carbapenemase genes (blaNDM-1, blaVIM, blaIMP, blaKPC, blaOXA-48/blaOXA-181) among imipenem resistant strains among the isolated Escherichia coli and Klebsiella spp. were detected by PCR. Sequencing was done to differentiate blaOXA-181 from blaOXA-48. Thirty-seven (22.29%) imipenem resistant isolates were detected during disk-diffusion technique, among them 43.24% carbapenemase producers were detected by MHT and 62.16% by PCR. NDM-1 (43.24%) was the dominant genotype followed by KPC (21.62%) and OXA-181 (18.92%). The results of this study showed presence of high proportion of carbapenemase enzyme producing Escherichia coli and Klebsiella spp. in Bangladesh. BlaOXA-181 gene is emerging in Bangladesh.
Key Words: Escherichia coli, Klebsiella spp., Carbapenemase, BlaOXA-181 gene and Bangladesh
MLA
Satter, S. et al. “OXA-181- an emerging threat in Bangladesh.” Journal of Molecular Studies and Medicine Research 03(01) (2018): 128-134.
APA
Satter, S., Mahbub, H. and Shamsuzzaman, S. M. (2018). OXA-181- an emerging threat in Bangladesh. Journal of Molecular Studies and Medicine Research, 03(01), 128-134.
Chicago
Satter, S., Mahbub, H. and Shamsuzzaman, S. M. “OXA-181- an emerging threat in Bangladesh.” Journal of Molecular Studies and Medicine Research 03(01) (2018): 128-134.
Harvard
Satter, S., Mahbub, H. and Shamsuzzaman, S. M. 2018. OXA-181- an emerging threat in Bangladesh. Journal of Molecular Studies and Medicine Research, 03(01), pp. 128-134.
Vancouver
Satter, S., Mahbub, H. Shamsuzzaman, SM. OXA-181- an emerging threat in Bangladesh. Journal of Molecular Studies and Medicine Research. 2018 October 03(01):128-134.
Satter, S. et al. “OXA-181- an emerging threat in Bangladesh.” Journal of Molecular Studies and Medicine Research 03(01) (2018): 128-134.
APA
Satter, S., Mahbub, H. and Shamsuzzaman, S. M. (2018). OXA-181- an emerging threat in Bangladesh. Journal of Molecular Studies and Medicine Research, 03(01), 128-134.
Chicago
Satter, S., Mahbub, H. and Shamsuzzaman, S. M. “OXA-181- an emerging threat in Bangladesh.” Journal of Molecular Studies and Medicine Research 03(01) (2018): 128-134.
Harvard
Satter, S., Mahbub, H. and Shamsuzzaman, S. M. 2018. OXA-181- an emerging threat in Bangladesh. Journal of Molecular Studies and Medicine Research, 03(01), pp. 128-134.
Vancouver
Satter, S., Mahbub, H. Shamsuzzaman, SM. OXA-181- an emerging threat in Bangladesh. Journal of Molecular Studies and Medicine Research. 2018 October 03(01):128-134.
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