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J. Mol. Stud. Med. Res. | Volume 03, Issue 02, 148-160| https://doi.org/10.18801/jmsmr.030218.17
​Article type: Research article, Received: 27.08.2018, Revised: 24.11.2018, Date of Publication: 25 December 2018.

Potential use of endophytic bacterial and fungi as bio fertilizer to promote plant growth in tissue culture banana

Ruth Murunde, Irene Muriithi and Henry Wainwright
Real IPM Limited Company, kenya.

Abstract
Banana is of special need to human society and are ranked as fourth most important food in the word after rice, maize. In Kenya, production of banana is constrained by among others declining soil fertility due to repeatedly application of fertilizers. Sustainable complementary response to declining soil fertility would be to increase the biological inputs of nutrients by exploitation of microorganisms. Endophytes which are mutualistic symbionts living asymptomatically within plant tissues have been reported to have beneficial effects on plant growth, therefore the effect of three endophytic Bacterial isolate (Bacillus subtilis and Serratia nematodhiphila), Fungal isolate (Trichoderma asperrellum), originating from Kenya were evaluated by inoculating on Grand Naine and William Hybrid banana cultivars under greenhouse condition at weaning stage and after two months for fifteen weeks. Plant responses to endophyte treatment was assessed on plant height, girth, number of functional leaves, fresh and dry roots weight. Improved growth of all parameters was observed for plants inoculated with endophytes when compared to the control. The study shows that the endophytes tested as growth promoters were found to have a significant effect in both cultivar plantlets. All treatments showed promising growth promoting properties. Isolate TR (Trichoderma asperellum) induced the largest increases in plant height 129.2cm (Graid Naine)  114.0cm ( William Hybrid) ) at 15th  week , however  all the treatment did not differ significantly in other growth parameter (Pseudo stem diameter, and total number of leaves ) at P= 0.05. The study shows that endophytes have potential to enhance growth of tissue-cultured banana plants. 
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Key Words: Musa spp. Serratia Nematodhiphila, Trichoderma asperellum, Graid Naine and William Hybrid     

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MLA
Murunde et al. “Potential use of endophytic bacterial and fungi as bio fertilizer to promote plant growth in tissue culture banana.” Journal of Molecular Studies and Medicine Research 03(02) (2018): 148-160.
 
APA
Murunde, R. Muriithi, I. and Wainwright, H. (2018). Potential use of endophytic bacterial and fungi as bio fertilizer to promote plant growth in tissue culture banana. Journal of Molecular Studies and Medicine Research, 03(02), 148-160.
 
Chicago
Murunde, R. Muriithi, I. and Wainwright, H. “Potential use of endophytic bacterial and fungi as bio fertilizer to promote plant growth in tissue culture banana.” Journal of Molecular Studies and Medicine Research 03(02) (2018): 148-160.
 
Harvard
Murunde, R. Muriithi, I. and Wainwright, H. 2018. Potential use of endophytic bacterial and fungi as bio fertilizer to promote plant growth in tissue culture banana. Journal of Molecular Studies and Medicine Research, 03(02), pp. 148-160.
 
Vancouver
Murunde, R, Muriithi, I and Wainwright, H. Potential use of endophytic bacterial and fungi as bio fertilizer to promote plant growth in tissue culture banana. Journal of Molecular Studies and Medicine Research. 2018 December 03(02):148-160.

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