J. Mol. Stud. Med. Res. | Volume 03, Issue 02, 161-168| https://doi.org/10.18801/jmsmr.030218.18
Article type: Research article, Received: 10.06.2018, Revised: 29.09.2018, Date of Publication: 25 December 2018.
Article type: Research article, Received: 10.06.2018, Revised: 29.09.2018, Date of Publication: 25 December 2018.
Inhibitory effects of Japanese plant leaf extracts on α-glucosidase activity
Tamanna Niger 1, Kazuhiro Ohtani 1 and Bhuiyan Feroze Ahamed 2
1 Laboratory of Human Health and Medical Science, Graduate School of Kuroshio Science, Kochi University, Japan
2 Research Institute of Molecular Genetics, Kochi University, Japan.
1 Laboratory of Human Health and Medical Science, Graduate School of Kuroshio Science, Kochi University, Japan
2 Research Institute of Molecular Genetics, Kochi University, Japan.
Abstract
In this study, methanol extracts of the leaves of nineteen Japanese plant species for which there have been no previous reports regarding in vitro α-glucosidase activities, were tested for their potential α-glucosidase inhibitory activities. Five plants, Quercus phillyraeoides, Mallotus japonicus, Sapium sebiferum, Elaeocarpus sylvestris var. ellipticus, and Myrica rubra showed high inhibitory activity against α-glucosidase with IC50 values in the range of 22 – 92 μg/ml. The methanol extracts of these five plants at 1 mg/ml showed significant inhibitory activity against rat intestinal sucrase. The hexane, ethyl acetate, butanol, and water extracts from the five plants were also screened for yeast α-glucosidase and rat intestinal sucrase inhibitory activity. The extracts of Q. phillyraeoides leaves (ethyl acetate and butanol extracts), M. japonicas leaves (ethyl acetate and butanol extracts), and E. sylvestris var. ellipticus leaves (ethyl acetate and butanol extracts) showed excellent inhibitory effects against yeast α-glucosidase. Methanol, ethyl acetate, and butanol extracts of the five plants also exhibited potent inhibitory effects against rat intestinal sucrase. Due to their inhibitory effect, the leaves of these five plant species were selected for further investigation to isolate and identify the active constituent(s) responsible for the potential antidiabetic activity.
Key Words: Diabetes, α-glucosidase inhibitor and Diabetes mellitus
In this study, methanol extracts of the leaves of nineteen Japanese plant species for which there have been no previous reports regarding in vitro α-glucosidase activities, were tested for their potential α-glucosidase inhibitory activities. Five plants, Quercus phillyraeoides, Mallotus japonicus, Sapium sebiferum, Elaeocarpus sylvestris var. ellipticus, and Myrica rubra showed high inhibitory activity against α-glucosidase with IC50 values in the range of 22 – 92 μg/ml. The methanol extracts of these five plants at 1 mg/ml showed significant inhibitory activity against rat intestinal sucrase. The hexane, ethyl acetate, butanol, and water extracts from the five plants were also screened for yeast α-glucosidase and rat intestinal sucrase inhibitory activity. The extracts of Q. phillyraeoides leaves (ethyl acetate and butanol extracts), M. japonicas leaves (ethyl acetate and butanol extracts), and E. sylvestris var. ellipticus leaves (ethyl acetate and butanol extracts) showed excellent inhibitory effects against yeast α-glucosidase. Methanol, ethyl acetate, and butanol extracts of the five plants also exhibited potent inhibitory effects against rat intestinal sucrase. Due to their inhibitory effect, the leaves of these five plant species were selected for further investigation to isolate and identify the active constituent(s) responsible for the potential antidiabetic activity.
Key Words: Diabetes, α-glucosidase inhibitor and Diabetes mellitus
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MLA
Niger et al. “Inhibitory effects of Japanese plant leaf extracts on α-glucosidase activity.” Journal of Molecular Studies and Medicine Research 03(02) (2018): 161-168.
APA
Niger, T. Ohtani, K. and Ahamed, B. F. (2018). Inhibitory effects of Japanese plant leaf extracts on α-glucosidase activity. Journal of Molecular Studies and Medicine Research, 03(02), 161-168.
Chicago
Niger, T. Ohtani, K. and Ahamed, B. F. “Inhibitory effects of Japanese plant leaf extracts on α-glucosidase activity.” Journal of Molecular Studies and Medicine Research 03(02) (2018): 161-168.
Harvard
Niger, T. Ohtani, K. and Ahamed, B. F. 2018. Inhibitory effects of Japanese plant leaf extracts on α-glucosidase activity. Journal of Molecular Studies and Medicine Research, 03(02), pp. 161-168.
Vancouver
Niger, T, Ohtani, K and Ahamed, BF. Inhibitory effects of Japanese plant leaf extracts on α-glucosidase activity. Journal of Molecular Studies and Medicine Research. 2018 December 03(02):161-168.
Niger et al. “Inhibitory effects of Japanese plant leaf extracts on α-glucosidase activity.” Journal of Molecular Studies and Medicine Research 03(02) (2018): 161-168.
APA
Niger, T. Ohtani, K. and Ahamed, B. F. (2018). Inhibitory effects of Japanese plant leaf extracts on α-glucosidase activity. Journal of Molecular Studies and Medicine Research, 03(02), 161-168.
Chicago
Niger, T. Ohtani, K. and Ahamed, B. F. “Inhibitory effects of Japanese plant leaf extracts on α-glucosidase activity.” Journal of Molecular Studies and Medicine Research 03(02) (2018): 161-168.
Harvard
Niger, T. Ohtani, K. and Ahamed, B. F. 2018. Inhibitory effects of Japanese plant leaf extracts on α-glucosidase activity. Journal of Molecular Studies and Medicine Research, 03(02), pp. 161-168.
Vancouver
Niger, T, Ohtani, K and Ahamed, BF. Inhibitory effects of Japanese plant leaf extracts on α-glucosidase activity. Journal of Molecular Studies and Medicine Research. 2018 December 03(02):161-168.
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