In vitro growth and multiplication of a hybrid orchid (Dendrobium alba x Ascanda dongtarm) with different concentration of plant growth regulators
M. N. Islam, K. M. Nasiruddin and M. Al Amin
Abstract
In vitro growth and multiplication of a hybrid orchid was carried out to investigate the effect of different plant growth regulators on development of protocorm like bodies (PLBs) and subsequent plantlets of hybrid orchid. Protocorm like bodies (PLBs) were cultured on Murashige and Skoog (MS) media containing different concentration of NAA (0, 0.5, 1.0, 1.5 and 2.0 mg/l), IAA (0, 0.5, 1.0, 1.5 and 2.0 mg/l) and 0.5 mg/l of Kinetin in all cases were used. Among the different concentration treatments of NAA and IAA; and Kinetin concentration, maximum weight of PLBs (1.37g/explant) were noticed on MS medium supplemented with 1.5 mg/l NAA, and the highest number of plantlets (18.58/explant) were observed on MS medium containing 1.0 mg/l NAA. But maximum PLBs multiplication and or highest number of PLBs (28.50/explant) were found on MS medium supplemented with 2.0 mg/l IAA+0.5 mg/l of Kinetin.
Key words: Hybrid orchid, Protocorm like bodies, In vitro multiplication and growth regulators
In vitro growth and multiplication of a hybrid orchid was carried out to investigate the effect of different plant growth regulators on development of protocorm like bodies (PLBs) and subsequent plantlets of hybrid orchid. Protocorm like bodies (PLBs) were cultured on Murashige and Skoog (MS) media containing different concentration of NAA (0, 0.5, 1.0, 1.5 and 2.0 mg/l), IAA (0, 0.5, 1.0, 1.5 and 2.0 mg/l) and 0.5 mg/l of Kinetin in all cases were used. Among the different concentration treatments of NAA and IAA; and Kinetin concentration, maximum weight of PLBs (1.37g/explant) were noticed on MS medium supplemented with 1.5 mg/l NAA, and the highest number of plantlets (18.58/explant) were observed on MS medium containing 1.0 mg/l NAA. But maximum PLBs multiplication and or highest number of PLBs (28.50/explant) were found on MS medium supplemented with 2.0 mg/l IAA+0.5 mg/l of Kinetin.
Key words: Hybrid orchid, Protocorm like bodies, In vitro multiplication and growth regulators
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Citation for this article:
Islam, M. N., Nasiruddin, K. M. & Amin, M. A. (2014). In vitro growth and multiplication of a hybrid orchid (Dendrobium alba x Ascanda dongtarm) with different concentration of plant growth regulators. Journal of Bioscience and Agriculture Research, 01(01), 29-35.
Islam, M. N., Nasiruddin, K. M. & Amin, M. A. (2014). In vitro growth and multiplication of a hybrid orchid (Dendrobium alba x Ascanda dongtarm) with different concentration of plant growth regulators. Journal of Bioscience and Agriculture Research, 01(01), 29-35.
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