Journal of Bioscience and Agriculture Research |
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Title: BAP and IBA pulsing for in vitro multiplication of banana cultivars through shoot-tip culture
M. H. Ferdous (a), A. A. Masum Billah (b), H. Mehraj (c), T. Taufique (d) and A. F. M. Jamal Uddin (d)
(a) Dept. of Genetics and Plant Breeding, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh
(b) Dept. of Agriculture Extension, Ministry of Agriculture, Iswardi, Pabna, Bangladesh
(c) The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarami, Matsumaya, Ehime 790-8556, Japan
(d) Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh
M. H. Ferdous (a), A. A. Masum Billah (b), H. Mehraj (c), T. Taufique (d) and A. F. M. Jamal Uddin (d)
(a) Dept. of Genetics and Plant Breeding, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh
(b) Dept. of Agriculture Extension, Ministry of Agriculture, Iswardi, Pabna, Bangladesh
(c) The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarami, Matsumaya, Ehime 790-8556, Japan
(d) Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh
| bap_and_iba_pulsing_for_in_vitro_multiplication_of_banana_cultivars_through_shoot-tip_culture-(v._1.0).pdf |
Abstract
The study was undertaken with a view to establish a protocol for in vitro plant regeneration from shoot tip explants of banana. Different concentrations of BAP (0.0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0 and 7.0 mg/l) and IBA (0.0, 1.0, 2.0, 3.0, 4.0 and 5.0 mg/l) was used in MS medium to assess the influence on in vitro shoot regeneration and subsequent root formation of Amritasagar and Sabri banana cultivars. Maximum single shoot formation (50.0% and 30.0%), number of single shoot (3.50 and 2.00 in), longest shoot (2.64 cm and 2.16 cm) were found from 0.5 mg/l BAP while maximum number of roots (3.83 and 2.50) and longest root (3.60 cm and 3.10 cm) was found from 0.3 mg/l IBA in Amritasagar and Sabri respectively. The survival of the plantlets of both cultivars was more than 82% under ex vitro condition. 0.5 mg/l BAP and 0.3 mg/l IBA can be used with MS media for shoot and root formation of Amritasagar and Sabri banana cultivars through shoot tip culture.
Keywords: Banana, growth regulators, in vitro shoot-tip culture and plantlets regeneration
Please cite this article as:
Ferdous, M. H., Masum Billah, A. A., Mehraj, H., Taufique, T. & Jamal Uddin, A. F. M. (2015). BAP and IBA pulsing for in vitro multiplication of banana cultivars through shoot-tip culture. Journal of Bioscience and Agriculture Research 03(02): 87-95.
The study was undertaken with a view to establish a protocol for in vitro plant regeneration from shoot tip explants of banana. Different concentrations of BAP (0.0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0 and 7.0 mg/l) and IBA (0.0, 1.0, 2.0, 3.0, 4.0 and 5.0 mg/l) was used in MS medium to assess the influence on in vitro shoot regeneration and subsequent root formation of Amritasagar and Sabri banana cultivars. Maximum single shoot formation (50.0% and 30.0%), number of single shoot (3.50 and 2.00 in), longest shoot (2.64 cm and 2.16 cm) were found from 0.5 mg/l BAP while maximum number of roots (3.83 and 2.50) and longest root (3.60 cm and 3.10 cm) was found from 0.3 mg/l IBA in Amritasagar and Sabri respectively. The survival of the plantlets of both cultivars was more than 82% under ex vitro condition. 0.5 mg/l BAP and 0.3 mg/l IBA can be used with MS media for shoot and root formation of Amritasagar and Sabri banana cultivars through shoot tip culture.
Keywords: Banana, growth regulators, in vitro shoot-tip culture and plantlets regeneration
Please cite this article as:
Ferdous, M. H., Masum Billah, A. A., Mehraj, H., Taufique, T. & Jamal Uddin, A. F. M. (2015). BAP and IBA pulsing for in vitro multiplication of banana cultivars through shoot-tip culture. Journal of Bioscience and Agriculture Research 03(02): 87-95.
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