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Volume 03 - Issue 01  |  Year of Publication: 2015           

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Article type: Research paper | No. 25 | Country: Bangladesh | pp. 01-07 | Downloads: 
Title: Comparison of Growth and Yield Characteristics of BARI Tomato Varieties
Authors: Biswas, M., Sarkar, D. R., Asif, M. I., Sikder, R. K, Mehraj H. & Jamal Uddin, A. F. M. 

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Abstract: An experiment was conducted at Agronomy Farm of the Sher-e-Bangla Agricultural University, Dhaka to study growth and yield responses of tomato varieties. Experiment consisted of four varieties, viz. BARI Tomato-4 (V1), BARI Tomato-5 (V2), BARI Tomato-7 (V3) and BARI Tomato-9 (V4) using Randomized Complete Block Design with three replications. Tallest plant (101.3 cm), maximum number of leaves (114.1/plant) and maximum number of branches (10.0/plant) was found from BARI Tomato-7. While maximum number of flowers (6.1/cluster), number of fruits (5.0/cluster), number of clusters (17.9/plant) were found from BARI Tomato-9. However, maximum fruit diameter (20.1 cm), individual fruit weight (115.9 g), yield (34.7 kg/plot and 95.9 t/ha), number of locule (4.4/fruit) were also found from BARI Tomato-7. Virus infestation, fruit length and Total soluble solid (TSS) were statistically identical among the varieties. Key words: Tomato, varieties, growth and yield

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Article type: Research paper | No. 26 | Country: Bangladesh | pp. 08-14 | Downloads: ​
Title: Effect of Growth Regulators on Germination and Vigor of Lentil Seeds
Authors: Pulok, M. A. I., Rahman, M. M., Haque, M. N., Chakraborty, R. & Ali, M. S.

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Abstract: An experiment was carried out to evaluate the effect of growth regulators viz., Gibberellin (GA3), lemon juice and tamarind leaf extract on germination and vigor of lentil seeds. The experiment was conducted with BARI Masur-4 cultivar, and three growth regulators: gibberellin, lemon juice and tamarind leaf extract were used. Three concentrations of gibberellins (10, 20 and 30 ppm), three concentrations of lemon juice (2, 4 and 6 %) and three concentrations of tamarind leaf extract (0.05, 0.1 and 0.15%) with two soaking time duration: 6 and 12 hours and control were used. Gibberellin (GA3) improved germination percentage, seedlings length, dry matter, germination index and vigor index of lentil. Lemon juice enhanced germination percentage, seedling length, vigor index and germination index in lentil. Tamarind leaf extract increased the germination percentage, vigor index and speed of germination in lentil. In comparison with plant growth regulators, length and weight was found to be positively correlated and in most cases relationships were significant. Key words: Lentil, growth regulators, germination and vigor

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Article type: Research Paper | No. 27 | Country: Bangladesh | pp. 15-27 | Downloads: ​
Title: Effect of NaCl Salt on Vegetative Growth and Yield of Sixteen Tomato Lines
Authors: Shiam, I. H., Nahiyan, A. S. M., Momena, K., Mehraj, H. & Jamal Uddin, A. F. M. 

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Abstract: An experiment was conducted at the Sher-e-Bangla Agricultural University, Bangladesh to evaluate influence of salt (NaCl) on sixteen tomato lines. Sixteen lines coded from V1 (Line-01) to V16 (Line-16) were executed under different NaCl salinity conditions (S0: Control; S1: 12dS/m and S2: 16 dS/m) following completely randomized design with three replications. Apart from control, V8 provided tallest plant in 12 dS/m (43.7 cm) and in 16 dS/m (38.4 cm) salinity level at 60 days after transplanting which was statistically similar with the V9 tomato line. V8 line provided the maximum number of leaves per plant except control (24.2 and 21.1 in 12 dS/m and 16 dS/m respectively). V9 line produced maximum leaf area (123.7 cm2 and 97.6 cm2 in 12dS/m and 16 dS/m respectively) under saline conditions which was followed by V8 line (112.7 cm2 and 92.6 cm2 in 12dS/m and 16 dS/m respectively). Maximum number of bunch per plant was observed from V9 line (10.7 and 9.3 in 12 dS/m and 16 dS/m respectively) followed by V8 line (9.3 and 9.3 in 12 dS/m and 16 dS/m respectively) except control. Maximum yield was found from V9 line (0.923 kg/plant) followed by V2 line (0.493 kg/plant) in 12 dS/m salinity level and maximum yield was found fromV9 line (0.593 kg/plant) which was closely followed by V8 line (0.407 kg/plant) in 16 dS/m salinity level. Tomato line-09 was found the best tomato cultivar for salt affected areas in Bangladesh. Key words: Tomato lines, salinity, growth and yield attributes

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Article type: Research Paper | No. 28 | Country: Bangladesh | pp. 28-37 | Downloads: ​
Title:  
Authors: 

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Abstract: ................................................................................................ Key words:

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Notice: This article has been removed upon request from authors.

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Article type: Research Paper | No. 29 | Country: Bangladesh | pp. 38 - 44 | Downloads: ​
Title: Performance of Mangrove Species Planted inside Sonneratia apetala Buch.-Ham. Plantations in the Coastal Belt of Bangladesh
Authors: Islam, S. A., Miah, M. A. Q. and Habib, M. A. 

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Abstract: An elimination trial with eleven mangrove species was conducted inside Sonneratia apetala Buch.-Ham. plantations at Rangabali island of Patuakhali district and Char Kukri-Mukri island of Bhola district, Bangladesh to observe the site-suitability and growth performance of the species. The data on survival, height and diameter growth were recorded from 21 years old matured experimental stands. Some mangrove species showed promising growth performance in the coastal belt. The highest survival was found in Aegiceras corniculatum followed by Phoenix paludosa, Excoecaria agallocha, Heritiera fomes and Xylocarpus mekongensis at Rangabali island. In the Char Kukri-Mukri island, the highest survival was recorded for E. agallocha followed by P. paludosa, A. corniculatum, H. fomes and X. mekongensis. Considering the height and diameter growth, the most promising species was E. agallocha and then X. mekongensis, A. corniculatum, H. fomes and P. paludosa in both the islands. Therefore, these mangrove species can be planted inside S. apetala plantations for sustainability of coastal forests in Bangladesh. Key words: Coastline, mangrove plantation, underplanting, survival and growth

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Article type: Research Paper | No. 30 | Country: Bangladesh | pp. 45-58 | Downloads: ​
Title: Screening of Rice Varieties for Bacterial Leaf Blight Resistance by Using SSR Markers
Authors: Khan, T. H., Evamoni, F. Z., Rubel M. H., Nasiruddin, K. M. & Rahman, M. 

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Abstract: Genetic variations of seventeen rice germplasms were studied using two SSR markers (RM122 and RM390) linked to the rice bacterial leaf blight (BLB) resistance genes.The average number of allele per locus was 10, with a range of 7 (RM122) to as many as 13 (RM390). The highest level of gene diversity value (0.9135) was observed in loci RM390 and the lowest level of gene diversity value (0.7059) was observed in loci RM122 with a mean diversity of 0.8097. The PIC values ranged from a low of 0.6715 (RM122) to a high of 0.9069 (RM390) and averaged of 0.7892. A dendrogram based on genetic distance using UPGMA, indicates segregation of the 17 rice varieties in six major clusters, BR10 and Binadhan 5, which were single variety formed cluster-1 and cluster-2 respectively. BRRIdhan 38, Malshira, Binadhan 7, BRRIdhan 29, BRRIdhan 31 were grouped in cluster-3, in which sub cluster-I include BRRIdhan 38, Malshira and sub cluster-II includes Binadhan 7, BRRIdhan 29, BRRIdhan 31. Cluster-4 was the largest cluster comprised of seven rice varieties. These are Iratom 24, BRRIdhan 34, Binasail, Binadhan 4, BR 26, BR 16 and Binadhan 6. Only BRRIdhan 33 was formed cluster-5. BR 14 and BRRIdhan 32 formed cluster-6. Thus, using the same linked marker RM122, BR 14 and BR 10 might be resistant to BLB diseases. SSR markers offer a potential, simple, rapid and reliable method in marker assisted breeding. Key words: Screening, BLB, Resistance, Rice varieties and SSR markers

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Article type: Research Paper | No. 31 | Country: Bangladesh | pp. 59-64 | Downloads: ​
Title: Cloning and expression of Cadherin fragment from Helicoverpa armigera 
Authors: Momena, K., Kumar, P. A., Mehraj, H. & Jamal Uddin, A. F. M.

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Abstract: Helicoverpa armigera is an important pest of agricultural crops which causes severe damage to the fruits and pods of crop plants, larval stage which causes this kind of damage. Helicoverpa armigera cadherin fragment contains its toxin binding region which enhance cry1Ac activity against Helicoverpa armigera larvae because this kind of interaction between HaCad1 receptors and cry1Ac induces toxin oligomerization. The aim of current investigation was to clone and expression the cadherin fragment. Cadherin gene was isolated from mid gut portion of Helicoverpa armigera and cloned into protein expression vector pMALc2x for expression study. The recombinant clone was transformed into protein expression host and protein was isolated and analysed the expected kDa by SDS- PAGE and Western blotting. The protein band was 70 kDa that confirmed 26.9 kDa cadherin protein was fused with 42.5 kDa Maltose binding protein. Present study successfully cloned and expressed cadherin fragment HaCad1 and Cry1Ac protein in Helicoverpa armigera. Our result provided a tactic for cadherin gene cloning and transformation into cry1Ac transgenic plants that can significantly enhance the insecticidal activity of Cry1Ac against Helicoverpa armigera. Key words: Helicoverpa armigera, Cry1Ac insecticidal protein and cadherin

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Volume 03 - Issue 02  |  Year of Publication: 2015          

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Article type: Research Paper | No. 32 | Country: Bangladesh | pp. 65-72 | Downloads: ​
Title: Effect of Farm Yard Manure, Gypsum and Nitrogen on Growth and Yield of Rice in Saline Soil of Satkhira District, Bangladesh
Authors:  Haque, A. N. A., Haque, M. E., Hossain, M. E., Khan, M. K. & Razzaque, A. H. M.

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Abstract: A field experiment was conducted at the sub-station farm of Bangladesh Institute of Nuclear Agriculture (BINA), Satkhira, to observe the effect of Farm yard manure (FYM), Gypsum and Nitrogen levels on growth and yield of rice (cv. Binadhan-8). The experiment was laid out in a RCBD split-plot design with three replications. Treatments consisted of five FYM and Gypsum combinations in main plot, which were S0: Control, S1: FYM 5 tha-1 + Gypsum 140 kgha-1, S2: FYM 5 tha-1 + Gypsum 210 kgha-1, S3: Gypsum 210 kgha-1 and S4: FYM 5 tha-1. Four nitrogen levels were assigned in sub-plots viz. N1: 50 kg Nha-1, N2: 75 kg Nha-1, N3: 100 kg Nha-1 and N4: 125 kg Nha-1. The study revealed that different FYM and Gypsum combinations along with different N levels have significant effect on growth and yield of rice. Mean effect of FYM and Gypsum combinations found the highest for grain (3.69 tha1) and straw (6.60 tha-1) yield where plot received FYM @ 5 tha-1 +Gypsum 210 kgha-1. Considering the mean effect of different N levels, when the plant received 125 kg Nha-1 then the maximum grain and straw yield of rice were observed as 3.81 and 6.91tha-1, respectively. Due to the interaction effect of both FYM and Gypsum combinations and N levels, the maximum grain yield of rice was found as 4.39 tha-1 under the treatment combination of S2N4, i.e., FYM @ 5 tha-1 and Gypsum 210 kgha-1along with 125 kg Nha-1. Key words: FYM, Nitrogen, Gypsum, Rice and Saline soil

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Article type: Research Paper | No. 33 | Country: Bangladesh | pp. 73-78 | Downloads: ​
Title: Effect of GA3 on Biochemical Attributes and Yield of Summer Tomato
Authors: Rahman, M. S., Haque, M. A. & Mostofa, M. G. 

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Abstract: An experiment was carried out in pots at Bangladesh Institute of Nuclear Agriculture, Bangladesh to evaluate influence of different concentrations of GA3 on biochemical parameters at different growth stages in order to maximize yield of summer tomato var. Binatomato-2. The concentrations of GA3 were 0, 25, 50, 75 and 100 ppm. They were applied at three stages, namely root soaking of seedlings before transplanting, vegetative and flowering stages. The experiment was laid out in a randomized complete block design with four replications. Results indicated that the highest chlorophyll and soluble protein contents were recorded when GA3 was applied through root soaking followed by vegetative stage and the lowest was found at the flowering stage. In contrast, the highest nitrate reductase activity was observed when GA3 was applied at the vegetative stage and the lowest activity was recorded at the flowering stage. The applications of 50-75 ppm GA3 had significantly encouraged the bio-chemical parameters studied at 50 DAT. The amount of GA3 applied at different stages had significant influence on the yield and yield attributes of summer tomato. The highest plant height was recorded when 50 ppm of GA3 was applied at the vegetative stage. While, the longest time to first fruit setting was required when the roots of the seedlings were soaked in 100 ppm GA3 solution. The application of 50 ppm GA3 by root soaking had significantly increased the number of flowers, fruits and fruit yield per plant but similar results were achieved when only 25 ppm GA3 was applied at the flowering stage. The fruit yield of tomato per plant increased linearly with the increased number of flowers and fruits per plant. Key words: Summer Tomato, Gibberellic Acid, Chlorophyll, Soluble protein and Nitrate reductase activity

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Article type: Research Paper | No. 34 | Country: Bangladesh | pp. 79-86 | Downloads: ​
Title: Isolation, Characterization and Selection of Rhizobium Strains for Cultivation of Sesbania sp. 
Author: Hasan, M. Z., Alam, M. S., Mian, M. H. & Islam, M. Z. 

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Abstract: The experiment was conducted to isolate Rhizobium form the root nodules of Deshi dhaincha (Sesbania aculeate) and African dhaincha (Sesbania rostrata), characterization of isolates for authentication, testing their performance as the inoculants and selection of better performed strains for future use as biofertilizer. Nineteen rhizobial strains were isolated from root nodule of dhaincha from 5 Agro Ecological Zones (AEZ 9, AEZ 11, AEZ 12, AEZ 13 and AEZ 28) of Bangladesh. The strains were characterized through morphological, biochemical, microscopic and growth observation. Experiment was conducted in bacteriologically controlled condition using sterile sand in plastic pot in glasshouse. Two varieties of Sesbania named Deshi dhaincha and African dhaincha were used as test crop varieties. Five plants were grown in each plastic pot. After 25 days of sowing plants were harvested and data on nitrogen content of shoot, shoot dry weight, root dry weight, nodule number and nodule dry weight of Sesbania cultivars in glass house were collected. Rhizobial inoculation had significant and positive effect on nitrogen content of shoot, shoot dry weight, root dry weight, nodule number and nodule dry weight for both varieties of Sesbania in sterile condition. The strain DhM3 recorded the highest nitrogen content of shoot, shoot dry weight, root dry weight, nodule number and nodule dry weight. The strain DhSk2 ranked second and DhSk4 ranked third in respect of growth, nodulation, nitrogen fixation and biomass production. Therefore, these three strains can be used for Rhizobium inocula production for cultivation of Sesbania but field level extended studies recommended Key words: Dhaincha, AEZ, Rhizobium, Sesbania sp., Nodule and Biofertilizer

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Article type: Research Paper | No. 35 | Country: Bangladesh | pp. 87-95 | Downloads: ​
Title: BAP and IBA pulsing for in vitro multiplication of banana cultivars through shoot-tip culture
Author: Ferdous, M. H., Masum Billah, A. A., Mehraj, H., Taufique, T. & Jamal Uddin, A. F. M.

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Abstract: The study was undertaken with a view to establish a protocol for in vitro plant regeneration from shoot tip explants of banana. Different concentrations of BAP (0.0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0 and 7.0 mg/l) and IBA (0.0, 1.0, 2.0, 3.0, 4.0 and 5.0 mg/l) was used in MS medium to assess the influence on in vitro shoot regeneration and subsequent root formation of Amritasagar and Sabri banana cultivars. Maximum single shoot formation (50.0% and 30.0%), number of single shoot (3.50 and 2.00 in), longest shoot (2.64 cm and 2.16 cm) were found from 0.5 mg/l BAP while maximum number of roots (3.83 and 2.50) and longest root (3.60 cm and 3.10 cm) was found from 0.3 mg/l IBA in Amritasagar and Sabri respectively. The survival of the plantlets of both cultivars was more than 82% under ex vitro condition. 0.5 mg/l BAP and 0.3 mg/l IBA can be used with MS media for shoot and root formation of Amritasagar and Sabri banana cultivars through shoot tip culture. Key words: Banana, growth regulators, in vitro shoot-tip culture and plantlets regeneration

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Article type: Survey Paper | No. 36 | Country: Bangladesh | pp. 96-103 | Downloads: ​
Title: Fertilizer use pattern on agriculture in Salua area of Chougachha upazila, Jessore, Bangladesh
Author: Rezaul Karim and Mst. Afia Aktar

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Abstract: Agriculture is one of the important issues of Bangladesh where a larger share of the people related to it for their livelihood, food and socio-economics. Farmers in Bangladesh mostly depend on chemical fertilizer within the auspicious of conventional agriculture for higher production without or little application of organic or compost fertilizers. Such a fertilizer management practice in agriculture not only causes massive deterioration of soil fertility but the farmers are also being hampered economically for higher price of chemical fertilizers. This experiment was conducted to focus mainly on the fertilizer usage and agricultural practices of the local farmers in Salua Area of Chougachha Upazila, Jessore district, Bangladesh. This study identified the reasons of using chemical fertilizers or causes for not using environmental friendly and beneficial compost in soils. This survey was conducted to know the insights of fertilizer usage pattern, and to identify causes why people are so much dependent on chemical fertilizer although it is expensive and sometimes harmful for soil, water and environment. The result of the present study showed that lack of knowledge, availability and lack of getting opportunity towards organic based fertilizers are the key reasons of not using compost fertilizer during cultivation of crops by the farmers. Key words: Agriculture, Chemical fertilizer, Compost fertilizer and Fertilizer application practice

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