Journal of Bioscience and Agriculture Research |
|
Research article:
In vitro callus induction and plantlet regeneration is influenced by the maturity status of embryos of Brassica rapa varieties
Sabrina Zisan, Arif Hassan Khan Robin, Ahasanul Hoque and Mohammad Rashed Hossain
Dept. of Genetics & Plant Breeding, Bangladesh Agricultural University (BAU), Mymensingh-2202, Bangladesh
J. bios. agric. res., volume 06, issue 01, pp. 518-529 | Available online: 18 December 2015
DOI: http://dx.doi.org/10.18801/jbar.060115.62
In vitro callus induction and plantlet regeneration is influenced by the maturity status of embryos of Brassica rapa varieties
Sabrina Zisan, Arif Hassan Khan Robin, Ahasanul Hoque and Mohammad Rashed Hossain
Dept. of Genetics & Plant Breeding, Bangladesh Agricultural University (BAU), Mymensingh-2202, Bangladesh
J. bios. agric. res., volume 06, issue 01, pp. 518-529 | Available online: 18 December 2015
DOI: http://dx.doi.org/10.18801/jbar.060115.62
62_jbar_in_vitro_callus_induction_and_plantlet_regeneration_is_influenced_by_the_maturity_status_of_embryos_of_brassica_rapa_varieties.pdf |
Abstract: Brassica rapa (AA, 2n = 20) is a prime oilseed species in Bangladesh that can be improved via biotechnological approaches. Rescue of developing embryos after an interspecific hybridization, which otherwise usually tend to abort, is very important for improvement of this species, that require an efficient embryo culture protocol. The immature torpedo shaped embryos (451-700 µm) and mature walking-stick type (>700 µm) embryos of five B. rapa varieties namely, Safal, Agrani, BINA Sarisha-6, BARI Sarisha-6, BARI Sarisha-15 were cultured in basal MS media supplemented with 1 mgL-1 BAP, 0.5 mgL-1 NAA & 1 mgL- 1 2,4-D. The immature embryo culture followed indirect somatic embryogenesis process but the mature embryos followed direct organogenesis. The immature embryos induced callus within 11-15 days whereas the mature embryos regenerated plantlets through direct organogenesis within 8-11 days. The varieties Agrani, BARI Sarisha-6 and BINA Sarisha-6 induced the highest percentages of callus from immature embryos and the varieties Safal and BARI Sarisha-15 regenerated the highest percentage of plantlet from mature embryos. Safal and Agrani induced callus and regenerated plantlets comparatively earlier than other varieties. An increase in the concentration of BAP from 10 to 20 ppm in MS media + 5 ppm IBA decreased the size of the plantlets of all varieties except the Agrani. The data could be used in improving the existing embryo rescue protocols. The direct organogenesis process of mature embryos can potentially shorten the breeding cycles.
Key words: Brassica rapa, embryo culture, callus, phytohormone, plantlet, 2’4-D and Basal MS media
Key words: Brassica rapa, embryo culture, callus, phytohormone, plantlet, 2’4-D and Basal MS media
APA (American Psychological Association)
Zisan, S., Robin, A. H. K., Hoque, A. & Hossain, M. R. (2015). In vitro callus induction and plantlet regeneration is influenced by the maturity status of embryos of Brassica rapa varieties. Journal of Bioscience and Agriculture Research, 06(01), 518-529.
MLA (Modern Language Association)
Zisan, S., Robin, A. H. K., Hoque, A. & Hossain, M. R. “In vitro callus induction and plantlet regeneration is influenced by the maturity status of embryos of Brassica rapa varieties.” Journal of Bioscience and Agriculture Research, 06.01 (2015): 518-529.
Chicago/Turabian
Zisan, S., Robin, A. H. K., Hoque, A. & Hossain, M. R. “In vitro callus induction and plantlet regeneration is influenced by the maturity status of embryos of Brassica rapa varieties.” Journal of Bioscience and Agriculture Research, 06, no. 01 (2015): 518-529.
Zisan, S., Robin, A. H. K., Hoque, A. & Hossain, M. R. (2015). In vitro callus induction and plantlet regeneration is influenced by the maturity status of embryos of Brassica rapa varieties. Journal of Bioscience and Agriculture Research, 06(01), 518-529.
MLA (Modern Language Association)
Zisan, S., Robin, A. H. K., Hoque, A. & Hossain, M. R. “In vitro callus induction and plantlet regeneration is influenced by the maturity status of embryos of Brassica rapa varieties.” Journal of Bioscience and Agriculture Research, 06.01 (2015): 518-529.
Chicago/Turabian
Zisan, S., Robin, A. H. K., Hoque, A. & Hossain, M. R. “In vitro callus induction and plantlet regeneration is influenced by the maturity status of embryos of Brassica rapa varieties.” Journal of Bioscience and Agriculture Research, 06, no. 01 (2015): 518-529.
- Ahmad, A. & Abdin, M. (2000). Interactive effect of sulphur and nitrogen on the oil and protein contents and on the fatty acid profiles of oil in the seeds of rapeseed (Brassica campestris L.) and mustard (Brassica juncea L. Czern. and Coss.). Journal of Agronomy and Crop Science, 185, http://dx.doi.org/10.1046/j.1439-037X.2000.00401.x
- Alam, M., Haque, M., Hossain, M., Sarker, S. & Afroz, R. (2009). Haploid plantlet regeneration through anther culture in oilseed Brassica species. Bangladesh Journal of Agricultural Research, 34, 693-703.
- Ara, J., Mahmud, J., Ryad, M., Nur, F. & Sarkar, S. (2014). Response of seed yield contributing characters and seed quality of rapeseed (Brassica campestris L.) to nitrogen and boron. Applied Science Reports, 15-10.
- BBS (2014). Bangaldesh Bureau of Statistics. Retrieved on 15 May, 2015.
- Bhuyan, M. A. A., Rahman, R., Robin, A. H. K. & Hassan, L. (2007). In vitro regeneration of three oilseed Brassica species through filament culture. Bangladesh Journal of Crop Science, 18, 295-300.
- Brown, J. & Brown, A. P. (1996). Gene transfer between canola (Brassica napus L. and B. campestris L.) and related weed species. Annals of Applied Biology, 129, 513-522. http://dx.doi.org/10.1111/j.1744-7348.1996.tb05773.x
- Burbulis, N., Blinstrubiene, A., Masiene, R. & Jonytiene, V. (2012). Influence of genotype, growth regulators and sucrose concentration on linseed (Linum usitatissimum L.) anther culture. Journal of Food Agriculture and Environment, 10, 764-767.
- Burbulis, N. & Kupriene, R. (2005). Induction of somatic embryos on in vitro cultured zygotic embryos of spring Brassica napus. Acta Universitatis Latviensis (Biology), 691, 137-143.
- Chi, H. S. (2000). Interspecific crosses of lily by in vitro pollinated ovules. Botanical Bulletin of Academia Sinica, 41.
- Choudhury, A., Saikia, M. & Dutta, K. (1990). Response of rapeseed (Brassica napus) to irrigation and nitrogen levels under sandy-loam soils of Assam. Indian Journal of Agricultural Sciences, 60, 347-349.
- Ćosić, T., Vinterhalter, B., Vinterhalter, D., Mitić, N. & Cingel, A. (2013). In vitro plant regeneration from immature zygotic embryos and repetitive somatic embryogenesis in kohlrabi (Brassica oleracea var. gongylodes). In Vitro Cellular & Developmental Biology-Plant, 49, 294-303. http://dx.doi.org/10.1007/s11627-013-9517-9
- Duncan, D., Williams, M., Zehr, B. & Widholm, J. (1985). The production of callus capable of plant regeneration from immature embryos of numerous Zea mays genotypes. Planta, 165, 322-332. http://dx.doi.org/10.1007/BF00392228
- Elhiti, M., Tahir, M., Gulden, R. H., Khamiss, K. & Stasolla, C. (2010). Modulation of embryo-forming capacity in culture through the expression of Brassica genes involved in the regulation of the shoot apical meristem. Journal of Experimental Botany, 61, 4069-4085. http://dx.doi.org/10.1093/jxb/erq222
- Ferrie, A. M. R., Palmer, C. E. & Keller, W. A. (1995). Haploid embryogenesis. In: Thorpe TA (ed) In vitro embryogenesis in plants. Kluwer Academic Publishers, Dordrecht, pp. 309-344. http://dx.doi.org/10.1007/978-94-011-0485-2_9
- Finkelstein, R. R. & Crouch, M. L. (1986). Rapeseed embryo development in culture on high osmoticum is similar to that in seeds. Plant Physiology, 81, 907-912. http://dx.doi.org/10.1104/pp.81.3.907
- George, L. & Rao, P. (1980). In vitro regeneration of mustard plants (Brassica juncea var. RAI-5) on cotyledon explants from non-irradiated, irradiated and mutagen-treated seed. Annals of Botany, 46, 107-112.
- Hilgert-Delgado, A., Klíma, M., Viehmannová, I., Urban, M. O. & Fernández-Cusimamani. E. (2015). Efficient resynthesis of oilseed rape (Brassica napus L.) from crosses of winter types B. rapa × B. oleracea via simple ovule culture and early hybrid verification. Plant Cell, Tissue and Organ Culture (PCTOC), 120, 191-201. http://dx.doi.org/10.1007/s11240-014-0593-2
- Inomata, N. (2012). Interspecific hybridization in brassica through ovary culture. Legumes and Oilseed Crops I, 10, 367-384.
- Islam, M. & Bari, M. (2014). Immature embryo is the potential source for in vitro plant regeneration in Jatropha curcas. Journal of Bio-Science, 20, 125-134. http://dx.doi.org/10.3329/jbs.v20i0.17726
- Khan, M., Robin, A. A. H. K., Nazim-Ud-Dowla, M., Talukder, S. & Hassan, L. (2010). In vitro regeneration potentiality of Brassica genotypes in differential growth regulators. Bangladesh Journal of Agricultural Research, 35, 189-199. http://dx.doi.org/10.3329/bjar.v35i2.5881
- Liu, C., Xu, Z. H. & Chua, N. H. (1993a). Auxin polar transport is essential for the establishment of bilateral symmetry during early plant embryogenesis. The Plant Cell Online, 5, 621-630. http://dx.doi.org/10.1105/tpc.5.6.621
- Liu, C. M., Xu, Z. H. & Chua, N. H. (1993b). Proembryo culture: in vitro development of early globular‐stage zygotic embryos from Brassica juncea. The Plant Journal, 3, 291-300. http://dx.doi.org/10.1111/j.1365-313X.1993.tb00179.x
- Maheswaran, G. & Williams, E. (1986). Primary and secondary direct somatic embryogenesis from immature zygotic embryos of Brassica campestris. Journal of Plant Physiology, 124, 455-463. http://dx.doi.org/10.1016/S0176-1617(86)80203-6
- Malek, M., Rafii, M., Samad, M. & Khatun, M. (2014). M8 rapeseed (Brassica napus L.) mutants: Evaluation for earliness with higher seed yield. Research on Crops, 15, 797-801. http://dx.doi.org/10.5958/2348-7542.2014.01414.4
- Malek, M., Rahman, L., Das, M., Hassan, L. & Rafii, M. (2013). Development of hexaploid' Brassica'(AABBCC) from hybrids (ABC) of 'Brassica carinata'(BBCC) x B. rapa (AA). Australian Journal of Crop Science, 7(9), 1375-1382.
- McVetty, P. B. & Duncan, R. W. (2015). Canola, Rapeseed, and Mustard: For Biofuels and Bioproducts. Industrial Crops. Springer, 133-156.
- Mila, A., Sarkar, A., Karim, N. & Islam, N. (2014). Yield response of a mustard mutant variety to different times of irrigation. Bangladesh Journal of Scientific Research, 26, 89-93. http://dx.doi.org/10.3329/bjsr.v26i1-2.20236
- Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue 456 cultures. Physiologia Plantarum, 15, 473-497. http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x
- Murata, M. & Orton, T. J. (1987). Callus initiation and regeneration capacities in Brassica species. Plant Cell, Tissue and Organ Culture, 11, 111-123. http://dx.doi.org/10.1007/BF00041844
- Özgen, M., Türet, M., Altınok, S. & Sancak, C. (1998). Efficient callus induction and plant regeneration from mature embryo culture of winter wheat (Triticum aestivum L.) genotypes. Plant Cell Reports, 18, 331-335. http://dx.doi.org/10.1007/s002990050581
- Pua, E-C., Trinh, T. & Chua, N-H. (1989). High frequency plant regeneration from stem explants of Brassica alboglabra Bailey in vitro. Plant Cell, Tissue and Organ Culture, 17, 143-152. http://dx.doi.org/10.1007/BF00046859
- Quazi, M. H. (1988). Interspecific hybrids between Brassica napus L. and B. oleracea L. developed by embryo culture. Theoretical and Applied Genetics, 75, 309-318. http://dx.doi.org/10.1007/BF00303970
- Raghavan, V. & Srivastava, P. (1982). Embryo culture. Experimental embryology of vascular plants. Springer. pp. 195-230. http://dx.doi.org/10.1007/978-3-642-67798-4_9
- Rahman, R., Bhuyan, M. A. A., Sultana, A., Robin, A. H. K. & Hassan, L. (2007). Filament culture of oilseed Brassica in B5 media. Bangladesh Journal of Crop Science, 18, 301–306.
- Rakow, G. (2004). Species origin and economic importance of Brassica. Brassica. Springer. pp. 3-11. http://dx.doi.org/10.1007/978-3-662-06164-0_1
- Ram Manohar, P., Pushpan, R. & Rohini, S. (2009). Mustard and its uses in Ayurveda. Indian Journal of Traditional Knowledge, 8, 400-404.
- Rao, M. M. & Sita, G. L. (1996). Direct somatic embryogenesis from immature embryos of rosewood (Dalbergia latifolia Roxb.). Plant Cell Reports, 15, 355-359. http://dx.doi.org/10.1007/BF00232371
- Robin, A. H. K., Nazim-Ud-Dowla, M. A. N., Khan, M. M. A. & Hassan, L. (2005). In vitro plant regeneration from anther derived calli of oilseed Brassica. Bangladesh Journal of Progressive Science & Technology, 3, 155–158.
- Robin, A. H. K., Hassan, L. & Quddus, M. A. (2005a). Effect of hormones and response of oilseed Brassica varieties on callus induction ability through anther culture. Bangladesh Society of Agriculture Science & Technology, 2, 29–32.
- Roy, A., Ghosh, S., Chaudhuri, M. & Saha, P. (2010). Effect of different plant hormones on callus induction in Gymnema sylvestris R. Br. (Asclepiadaceous). African Journal of Biotechnology, 7, 2209-2211.
- Sayem, M., Maniruzzaman, M., Siddique, S. & Al-Amin, M. (2010). In vitro shoot regeneration through anther culture of Brassica spp. Bangladesh Journal of Agricultural Research, 35, 331-341. http://dx.doi.org/10.3329/bjar.v35i2.5896
- Sayem, A. S. M., Robin, A. H. K., Raffi, S. A. & Hassan, L. (2006). In vitro regeneration of four oilseed Brassica varieties of different species through leaf segment culture. Bangladesh Journal of Crop Science, 17,169–173.
- Sears, R. & Deckard, E. (1982). Tissue culture variability in wheat: callus induction and plant regeneration. Crop Science, 22, 546-550. http://dx.doi.org/10.2135/cropsci1982.0011183X002200030027x
- Seyis, F. & Aydin, E. (2014). The last barrier for 00-type interspecific rapeseed (Brassica napus L.): Glucosinolates. Turkish Journal of Agricultural and Natural Sciences: Special Issue: 2, 1413-1418.
- Sharma, D., Kaur R. & Kumar, K. (1996). Embryo rescue in plants - a review. Euphytica, 89, 325-337.
- Siong, P., Taha, R. & Rahiman, F. (2011). Somatic embryogenesis and plant regeneration from hypocotyl and leaf explants of Brassica oleracea var. botrytis (cauliflower). Acta Biologica Cracoviensia Series Botanica, 53, 26-31. http://dx.doi.org/10.2478/v10182-011-0004-5
- Uma, S., Lakshmi, S., Saraswathi, M., Akbar, A. & Mustaffa, M. (2011). Embryo rescue and plant regeneration in banana (Musa spp.). Plant Cell, Tissue and Organ Culture (PCTOC), 05, 105-111. http://dx.doi.org/10.1007/s11240-010-9847-9
- Zhang, G., Zhou, W., Gu, H., Song, W. & Momoh, E. (2003). Plant regeneration from the hybridization of Brassica juncea and B. napus through embryo culture. Journal of Agronomy and Crop Science, 189, 347-350. http://dx.doi.org/10.1046/j.1439-037X.2003.00059.x
- Zou, J., Fu, D., Gong, H., Qian, W. & Xia, W. (2011). De novo genetic variation associated with retro transposon activation, genomic rearrangements and trait variation in a recombinant inbred line population of Brassica napus derived from interspecific hybridization with Brassica rapa. The Plant Journal, 68, 212-224. http://dx.doi.org/10.1111/j.1365-313X.2011.04679.x
© 2016 The Authors. The article published by Journal BiNET, it is available to read, share, download, print, permitted for share alike use and build upon, provided that specified full length article is obtained; and the original author(s) and publisher are given due credit.