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Journal of Bioscience and Agriculture Research

You are here: Home>JBAR Journal>Archive>Volume 24

Bioscience journal
Volume 24  - Issue 01  |  Year of Publication: 2020      

  • Article No. 241
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Article Type: Research Article | No. 241, 2020 | Country: Bangladesh | pp. 1969-1976 | Open Access
Title: Impact of SPAD 502 meter based N fertilization on growth and yield attributes of wheat
​Authors: Md. Zillur Rahman, Shirin Akter, Mozammel  Hoque,  Abdus Sadeque, Md. Masikur Rahman and Md. Rafiqul Islam
DOI:​ https://doi.org/10.18801/jbar.240120.241
Impact of SPAD 502 meter based N fertilization on growth and yield attributes of wheat
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Abstract
A field experiment was undertaken at the Agronomic Research Site of the Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh to optimize nitrogen application in wheat field using SPAD-502 Chlorophyll Meter. Four nitrogen (N) rates from 0 to 120 kg ha-1 and different timing of N application were maintained in the experiment to test the usefulness of SPAD threshold values in decision making for supplemental nitrogen supply in wheat. A non-fertilized treatment was compared with three fertilized treatments getting 20 kg ha-1 of N at the time of crop emergence. After that supplementary nitrogen was applied to the three fertilized treatments @ 20, 40 or 60 kg N ha-1 when the SPAD values fell below the critical value of 45 SPAD (dynamic fertilization of nitrogen). Tillering of wheat plant was strongly influenced by N supply and spike bearing tillering enhanced when adequate N was applied. Application up to 120 kg N ha-1 significantly increased the plant height, total dry matter production, number of effective tillers, crop growth rate, yield contributing attributes and SPAD chlorophyll index in wheat. On the other hand, only culm reserve translocation to grain was observed higher in control and gradually decreased with increasing the levels of nitrogen. The ability of the SPAD meter to detect treatment differences due to various nitrogenous doses varied with the growth stage. The SPAD reading at all stages is positively correlated with wheat yield. Thus, the timing of nitrogen application should be closely coincided with the plant need-based to optimize the productivity of wheat.

Keywords: Nitrogen, SPAD reading, Dry matter, Culm reserve and Yield.
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Impact of SPAD 502 meter based N fertilization on growth and yield attributes of wheat

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MLA
Rahman, et al. “Impact of SPAD 502 meter based N fertilization on growth and yield attributes of wheat”. Journal of Bioscience and Agriculture Research, 24(01) (2020): 1969-1976.
 
APA
Rahman, M. Z., Akter, S., Hoque, M., Sadeque, A., Rahman, M. M. and Islam, M. R. (2020). Impact of SPAD 502 meter based N fertilization on growth and yield attributes of wheat. Journal of Bioscience and Agriculture Research, 24(01), 1969-1976.
 
Chicago
Rahman, M. Z., Akter, S., Hoque, M., Sadeque, A., Rahman, M. M. and Islam, M. R. “Impact of SPAD 502 meter based N fertilization on growth and yield attributes of wheat”. Journal of Bioscience and Agriculture Research, 24(01) (2020): 1969-1976.
 
Harvard
Rahman, M. Z., Akter, S., Hoque, M., Sadeque, A., Rahman, M. M. and Islam, M. R. 2020. Impact of SPAD 502 meter based N fertilization on growth and yield attributes of wheat. Journal of Bioscience and Agriculture Research, 24(01), pp. 1969-1976.
 
Vancouver
Rahman, MZ, Akter, S, Hoque, M, Sadeque, A, Rahman, MM, Islam, MR. Impact of SPAD 502 meter based N fertilization on growth and yield attributes of wheat. Journal of Bioscience and Agriculture Research, 2020 April  24(01): 1969-1976.

  • Article No. 242
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Article Type: Research Article | No. 242, 2020 | Country: Kenya | pp. 1977-1989 | Open Access
Title: Reduced stomatal conductance and irradiance account for soybean [Glycine max (L.) Merrill] yield decline in maize-soybean intercrop
​Authors: Louis Hortensius Mwamlima, Erick Kimutai Cheruiyot and Josephine Pamela Ouma
DOI:​ https://doi.org/10.18801/jbar.240120.242
Reduced stomatal conductance and irradiance account for soybean [Glycine max (L.) Merrill] yield decline in maize-soybean intercrop

Abstract
An increase in human population and urbanization has reduced availability of agricultural land making intercropping a system of choice amongst farmers with small land holdings. A study to determine the effect of soybean [Glycine max (L.) Merrill] and maize (Zea mays) intercropping on the interception of photosynthetically active radiation, stomatal conductance and yield of soybean was conducted in Siaya, Busia and Nakuru counties in Kenya during 2018 season. The experiment was laid out in a randomized complete block design (RCBD) and had three replicates. It had seven treatments; sole maize, sole soybean, within row intercropping, 1M:1S row pattern, 2M: 2S row pattern, 2M: 1S row pattern, and 1M:2S row pattern. Collected data were analyzed using analysis variance (ANOVA) using the linear mixed model for RCBD with a factorial treatment arrangement in the Genstat 18th edition. Soybean leaf chlorophyll content under intercropping was 21.16% more than chlorophyll content attained under sole cropping. Intercropping reduced soybean stomatal conductance, interception of photosynthetically active radiation and grain yield by 42.15, 78.88 and 83.85% respectively, compared to mono-cropping. Intercropping of maize and soybean was more productive than sole cropping and that maize was a more competitive crop than soybean in the mixture. Planting maize and soybean in 1M: 1S row pattern led to relatively higher soybean yields compared to other row patterns and is recommended for intercropping of the two crops in Kenya.

Keywords: Intercropping, Nodulation, Photosynthesis, Row pattern and Sole cropping
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Reduced stomatal conductance and irradiance account for soybean [Glycine max (L.) Merrill] yield decline in maize-soybean intercrop

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MLA
Mwamlima et al. “Reduced stomatal conductance and irradiance account for soybean [Glycine max (L.) Merrill] yield decline in maize-soybean intercrop.” Journal of Bioscience and Agriculture Research, 24(01) (2020): 1977-1989.
 
APA
Mwamlima, L. H., Cheruiyot, E. K. and Ouma, J. P. (2020). Reduced stomatal conductance and irradiance account for soybean [Glycine max (L.) Merrill] yield decline in maize-soybean intercrop. Journal of Bioscience and Agriculture Research, 24(01), 1977-1989.
 
Chicago
Mwamlima, L. H., Cheruiyot, E. K. and Ouma, J. P. “Reduced stomatal conductance and irradiance account for soybean [Glycine max (L.) Merrill] yield decline in maize-soybean intercrop.” Journal of Bioscience and Agriculture Research, 24(01) (2020): 1977-1989.
 
Harvard
Mwamlima, L. H., Cheruiyot, E. K. and Ouma, J. P. 2020. Reduced stomatal conductance and irradiance account for soybean [Glycine max (L.) Merrill] yield decline in maize-soybean intercrop. Journal of Bioscience and Agriculture Research, 24(01), pp. 1977-1989.
 
Vancouver
Mwamlima LH., Cheruiyot EK. and Ouma, JP. Reduced stomatal conductance and irradiance account for soybean [Glycine max (L.) Merrill] yield decline in maize-soybean intercrop. 2020 April 24(01): 1977-1989.

  • Article No. 243
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Article Type: Research Article | No. 243, 2020 | Country: Bangladesh | pp. 1990-1998 | Open Access
Title: Effect of phytohormone on shoot generation potentiality in rucola
​Authors: Sadia Afrin, Md. Abu Zafur Al Munsur, Md. Shahidul Islam Khan, Fahima Khatun and M. E. Hoque
DOI:​ https://doi.org/10.18801/jbar.240120.243
Effect of phytohormone on shoot generation potentiality in rucola

Abstract
The present investigation was carried out to study the in vitro regeneration in Rucola. Different concentrations and combinations of BA and IBA were used for regeneration of root and shoot in rucola. Cotyledonary nodes were used as explants. The highest number of shoots (4.60) and leaves (6.4) were recorded in treatment 4.0 mg/l BA with minimum (9.00) days. The maximum days (14.00) was noticed in a simple MS medium to regenerate lower no. of shoots. The treatment BA 4.0 mg/l produced the highest number of the main root (4.60) while it was lowest in the control treatment. The highest number of shoot (3.40) was noticed from the BA 4.0 mg/l + IBA 3.0 mg/l. The treatment BA 4.0 mg/l+ IBA 4.0 mg/l gave the highest number of leaves (11.00) and maximum number of roots (3.60). The regenerated plantlet was acclimatized in natural pot and soil conditions. Finally, a convenient protocol technique has been established for in vitro regeneration of rucola, which can be used for large scale plantlet production.

Key Words: Phytohormone, Cotyledonary Nodes, Regeneration, Rucola, IBA and BA
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Effect of phytohormone on shoot generation potentiality in rucola

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MLA
Afrin, S. et al. “Effect of phytohormone on shoot generation potentiality in rucola”. Journal of Bioscience and Agriculture Research, 24(01), (2020): 1990-1998.
 
APA
Afrin, S., Munsur, M. A. Z. A., Khan, M. S. I., Khatun, F. and Hoque, M. E. (2020). Effect of phytohormone on shoot generation potentiality in rucola. Journal of Bioscience and Agriculture Research, 23(02), 1990-1998.

Chicago

Afrin, S., Munsur, M. A. Z. A., Khan, M. S. I., Khatun, F. and Hoque, M. E. “Effect of phytohormone on shoot generation potentiality in rucola”. Journal of Bioscience and Agriculture Research, 24(01), (2020): 1990-1998.
 
Harvard
Afrin, S., Munsur, M. A. Z. A., Khan, M. S. I., Khatun, F. and Hoque, M. E. 2020. Effect of phytohormone on shoot generation potentiality in rucola. Journal of Bioscience and Agriculture Research, 24(01), pp. 1990-1998.
 
Vancouver
Afrin, S, Munsur, MAZA, Khan, MSI, Khatun, F and Hoque, ME. Effect of phytohormone on shoot generation potentiality in rucola. Journal of Bioscience and Agriculture Research, 2020 April 24(01), 1990-1998.

  • Article No. 244
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Article Type: Research Article | No. 244, 2020 | Country: Nigeria | pp. 1999-2005 | Open Access
Title: Responses of broiler chickens fed varying levels of dietary waterleaf (Talinum triangulare)
​Authors: Ekine, Opuda Alioma., Orindu, Adaeze Naomi and Ukanwoko, Anthonia Ijeoma
DOI:​ https://doi.org/10.18801/jbar.240120.244
Responses of broiler chickens fed varying levels of dietary waterleaf (Talinum triangulare)

Abstract
This study was carried out to determine the responses of broiler chickens fed varying levels of dietary Waterleaf (Talinum trangulare). One hundred and twenty broiler chickens were used for this study. Waterleaf (Talinum trangulare) was introduced to the birds at four (4) weeks of age after they were randomly allotted into four (4) dietary treatments. Treatment 1, which was the control, had no Waterleaf (Talinum trangulare), Treatments 2, 3, and 4 had 100g, 200g and 300g of Waterleaf respectively, in a Completely Randomized Design (CRD). Water was given ad libitum. The data collected from this study showed that the final weight, weekly weight gain, weekly feed consumption and feed efficiency showed a significant difference (P<0.05) between the treatments, indicating a higher feed utilization by the birds in Treatment 4. However, the results obtained from the organ weights, showed significant effects (P<0.05) on the liver, with the highest value in treatment 4. The test on serum enzymes showed that Aspartate Amino Transferase (AST) and Alkaline Phosphate Transferase (ALP) showed significant differences (P<0.05)   between the treatments, while the serum lipid profile indicated a significant difference (P<0.05) in the High Density Lipoproteins (HDL). The analysis of the Blood electrolytes and blood chemistry showed significant differences (P<0.05) in Potassium (k), Sodium (Na), Bicarbonate and Conjugated Bilirubin amongst the treatments. The Lymphocyte counts showed a significant difference (P<0.05) amongst the treatment in the haematological analysis. From the results, it can be concluded that Waterleaf (Talinum trangulare) improves feed utilization, stimulates appetite and a good source of electrolytes. It is, therefore, recommended to supplement broiler diets with Waterleaf (Talinum trangulare).

Key Words
: Aspartate Amino Transferase, Feed Efficiency, High Density Lipoproteins, Lymphocytes and Potassium.
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Responses of broiler chickens fed varying levels of dietary waterleaf (Talinum triangulare)

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MLA
Ekine, O. A. et al. “Responses of broiler chickens fed varying levels of dietary waterleaf (Talinum triangulare)”. Journal of Bioscience and Agriculture Research, 24(01), (2020):1999-2005.
 
APA
Ekine, O. A., Orindu, A. N. and Ukanwoko A. I. (2020). Responses of broiler chickens fed varying levels of dietary waterleaf (Talinum triangulare). Journal of Bioscience and Agriculture Research, 24(01), 1999-2005.
 
Chicago
Ekine, O. A., Orindu, A. N. and Ukanwoko A. I. “Responses of broiler chickens fed varying levels of dietary waterleaf (Talinum triangulare)”. Journal of Bioscience and Agriculture Research, 24(01), (2020):1999-2005.
 
Harvard
Ekine, O. A., Orindu, A. N. and Ukanwoko A. I. 2020. Responses of broiler chickens fed varying levels of dietary waterleaf (Talinum triangulare). Journal of Bioscience and Agriculture Research, 24(01), pp. 1999-2005.
 
Vancouver
Ekine, OA, Orindu, AN and Ukanwoko, AI. Responses of broiler chickens fed varying levels of dietary waterleaf (Talinum triangulare). Journal of Bioscience and Agriculture Research. 2020 April 24(01), 1999-2005

  • Article No. 245
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Article Type: Research Article | No. 245, 2020 | Country: Bangladesh | pp. 2006-2011 | Open Access
Title: Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium)
​Authors: Md. Shahidul Islam Khan, Fahima Khatun, Sadia Afrin, Md. Abu Zafur Al Munsur and M. E. Hoque
DOI:​ https://doi.org/10.18801/jbar.240120.245
Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium)

Abstract
The present investigation was carried out to study the in vitro regeneration in chrysanthemum. MS medium supplemented with different concentrations and combinations of BA and IAA for regeneration and multiple shoot formations. Nodal segments were used as explants. The shoot induction percentage was highest (76%) in the treatment BA 2.0 mg/l+ IAA 1.0 mg/l. The same combination regenerated the highest number of shoot (3.40) with minimum (7.60) days. The maximum days (15.80) was noticed in a simple MS medium. The highest number of leaf (11.60) was observed in the treatment of 3.0 mg/l BA+ 1.0 mg/l IAA. The treatment BA 2.0 mg/l + IAA 1.0 mg/l produced the highest number of main root (4.20) while it was lowest in the control treatment. A robust and healthy plantlet was regenerated within 28 days of subculture in the treatment BA 2.0 mg/l + IAA 1.0 mg/l. The regenerated plantlet was acclimatized in natural pot and soil conditions. An approximate 75% plantlet was established in both systems. Finally, a convenient protocol technique has been established for in vitro regeneration of chrysanthemum which can be used for large scale plantlet production in chrysanthemum.
 
Key Words: Phytohormone, Regeneration, Chrysanthemum, BA, IAA, Shoot induction and Root Induction
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Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium)
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MLA
Khan, M. S. I. et al. “Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium)”. Journal of Bioscience and Agriculture Research, 24(01), (2020): 2006-2011.
 
APA
Khan, M. S. I., Khatun, F., Afrin, S., Munsur, M. A. Z. A. and Hoque, M. E. (2020). Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium). Journal of Bioscience and Agriculture Research, 24(01), 2006-2011.
 
Chicago
Khan, M. S. I., Khatun, F., Afrin, S., Munsur, M. A. Z. A. and Hoque, M. E. “Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium)”. Journal of Bioscience and Agriculture Research, 24(01), (2020): 2006-2011.
 
Harvard
Khan, M. S. I., Khatun, F., Afrin, S., Munsur, M. A. Z. A. and Hoque, M. E. 2020. Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium). Journal of Bioscience and Agriculture Research, 24(01), pp. 2006-2011.

Vancouver
Khan, MSI, Khatun, F, Afrin, S, Munsur, MAZA and Hoque ME. Combine effect of BA and IAA on shoot and root induction potentiality in chrysanthemum (Chrysanthemum morifolium). Journal of Bioscience and Agriculture Research, 2020 April 24(01): 2006-2011.

Volume 24 - Issue 02  |  Year of Publication: 2020    ​      

  • Article No. 246
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Article Type: Research Article | No. 246, 2020 | Country: Bangladesh | pp. 2012-2019 | Open Access
Title: Effect of salinity (NaCl) on germination and seedling growth of mungbean (Vigna radiata L.)
​Authors: Subroto Podder, Joyanti Ray, Debesh Das and Bidhan Chandro Sarker
DOI:​ https://doi.org/10.18801/jbar.240220.246​
Effect of salinity (NaCl) on germination and seedling growth of mungbean (Vigna radiata L.)
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Abstract
Soil salinity adversely affects crops at the critical stages of growth, which in severe cases causes total yield loss. So screening of salt tolerant genotypes is very important and it can be done during germination and early growth stage by quick and easy method. An experiment was conducted to investigate the effect of salinity on germination and seedling growth of mungbean genotypes. The experiment comprised two factors viz. genotypes (BARI Mung-6, Binamoog-5, Binamoog-8 and Tila Mung) and salinity levels (0 mM/L, 40 mM/L, 80 mM/L, 120 mM/L and 160 mM/L). Among the seed germination parameters, no significant genotypic difference observed at 0 mM /L and 40 mM/L. At 120 mM/L, the highest germination capacity (93%) and germination percentage (94%) was found in Binamoog-8 followed by BARI Mung-6 (91% and 92% respectively) and the lowest value was in Tila Mung (73% and 76% respectively). At 160 mM/L, BARI Mung-6 showed the highest germination capacity (95%) and germination percentage (95%) and Tila Mung showed the lowest value (51% and 52% respectively). At 160 mM/L, the highest shoot length (2.8 cm), seedling vigor index (488.26), seedling dry weight (45.9 mg) found in BARI Mung-6 while the lowest in Tila Mung (1.56 cm, 180.93 and 32.52 mg respectively). Regarding all the germination and seedling growth parameters, BARI Mung-6 was found superior to other genotypes. Study should be conducted in the field for better understanding the effects of salinity stress on growth and yield performance of mungbean.
 
Key Words: Mungbean, NaCl, Germination and Seedling growth
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Effect of salinity (NaCl) on germination and seedling growth of mungbean (Vigna radiata L.)
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MLA
Podder, et al. “Effect of salinity (NaCl) on germination and seedling growth of mungbean (Vigna radiata L.)”. Journal of Bioscience and Agriculture Research, 24(02), (2020): 2012-2019.
 
APA
Podder, S., Ray, J., Das, D. and Sarker, B. C. (2020). Effect of salinity (NaCl) on germination and seedling growth of mungbean (Vigna radiata L.). Journal of Bioscience and Agriculture Research, 24(02), 2012-2019.
 
Chicago
Podder, S., Ray, J., Das, D. and Sarker, B. C. “Effect of salinity (NaCl) on germination and seedling growth of mungbean (Vigna radiata L.)”. Journal of Bioscience and Agriculture Research, 24(02), (2020): 2012-2019.
 
Harvard
Podder, S., Ray, J., Das, D. and Sarker, B. C. 2020. Effect of salinity (NaCl) on germination and seedling growth of mungbean (Vigna radiata L.). Journal of Bioscience and Agriculture Research, 24(02), pp. 2012-2019.
 
Vancouver
Podder, S, Ray, J, Das, D, and Sarker, BC. Effect of salinity (NaCl) on germination and seedling growth of mungbean (Vigna radiata L.). Journal of Bioscience and Agriculture Research, 2020 May 24(02), 2012-2019.

  • Article No. 247
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Article Type: Research Article | No. 247, 2020 | Country: Bangladesh | pp. 2020-2024 | Open Access
Title: Effect of hyaluronic acid on the organogenesis of an Oncidium cultivar
​Authors: Shahrin Sharmin, Kazuhiko Shimasaki, A. F. M. Jamal Uddin and Md. Manirul Alam
DOI:​ https://doi.org/10.18801/jbar.240220.247
Effect of hyaluronic acid on the organogenesis of an Oncidium cultivar
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Abstract
Protocorm-like bodies (PLBs) multiplication is one of the most preferable in vitro methods to increase the number of orchids in a short time. The current investigation was conducted to understand the impact of two types of hyaluronic acid (HA12 and HA20) using various concentrations on the regeneration of Oncidium Aloha ‘Iwanaga’. The highest number of PLBs were found at 0.1mg/l of HA12 (20.7/explant) and HA20 (23.1/explant) respectively. The highest number of shoots were found at 0.1mg/l of HA12 (2.1/explant) and HA20 (3.5/explant) respectively. The highest formation rate of PLBs and shoots was found in both hyaluronic acids at 0.1mg/l. No root formation was observed. The highest fresh weight was obtained with 0.1mg/l HA12 (195.5mg) and 0.1mg/l HA20 (212.3mg) respectively. So use of hyaluronic acid could be a potential source of PLB proliferation within short time in Oncidium Aloha ‘Iwanaga’.
 
Key Words: Orchid, Protocorm-like bodies (PLBs), In vitro micropropagation and Organogenesis
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Effect of hyaluronic acid on the organogenesis of an Oncidium cultivar
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MLA
Sharmin, S. et al. “Effect of hyaluronic acid on the organogenesis of an Oncidium cultivar”. Journal of Bioscience and Agriculture Research, 24(02), (2020): 2020-2024.
 
APA
Sharmin, S., Shimasaki, K, Jamal Uddin, A. F. M. and Alam, MM (2020). Effect of hyaluronic acid on the organogenesis of an Oncidium cultivar. Journal of Bioscience and Agriculture Research, 24(02), 2020-2024.
 
Chicago
Sharmin, S., Shimasaki, K, Jamal Uddin, A. F.M. and Alam, M. M. “Effect of hyaluronic acid on the organogenesis of an Oncidium cultivar”. Journal of Bioscience and Agriculture Research, 24(02), (2020): 2020-2024.
 
Harvard
Sharmin, S., Shimasaki, K, Jamal Uddin, A. F. M. and Alam, M. M. 2020. Effect of hyaluronic acid on the organogenesis of an Oncidium cultivar. Journal of Bioscience and Agriculture Research, 24(02), pp. 2020-2024.
 
Vancouver
Sharmin, S, Shimasaki, K, Jamal Uddin, AFM and Alam, MM. Effect of hyaluronic acid on the organogenesis of an Oncidium cultivar. Journal of Bioscience and Agriculture Research, 2020 May 24(02), 2020-2024.

  • Article No. 248
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Article Type: Research Article | No. 248, 2020 | Country: Bangladesh | pp. 2025-2029 | Open Access
Title: In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs
​Authors: Shahrin Sharmin, Kazuhiko Shimasaki and A. F. M. Jamal Uddin
DOI:​ https://doi.org/10.18801/jbar.240220.248
In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs
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Abstract
Different developmental processes in plants are highly influenced by cytokinins.  On the other hand, recently light emitting diode has garnered much reputation in plant tissue culture system due to its high energy efficiency and versatility. In the present study the effective concentration of 6-benzylaminopurine (BAP) on the organogenesis of Oncidium Aloha ‘Iwanaga’ was assessed and further evaluated under different LEDs. From the initial experiment, 0.1mg/l BAP was found to be the optimum concentration among others in terms of the average number of PLBs/explant and other parameters. The highest number of PLBs (28.1/explant) and shoots (2.0/explant) was obtained using 0.1mg/l BAP. Regarding root formation no response was observed under the treatment applied. In case of the LED treatments with 0.1mg/l BAP, the trial indicated that the number of PLBs increased under white (28.1/explant) and blue (26.2/explant) LED. Maximum shoot regeneration and fresh weight were observed under white LED compared to control. So, a combination of 0.1mg/l BAP with white LED could be proposed to be suitable for in vitro regeneration of Oncidium Aloha ‘Iwanaga’.
 
Key Words: Orchid, Protocorm-like bodies, Shoot, Micropropagation and Organogenesis
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In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs​
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MLA
Sharmin, S. et al. “In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs”. Journal of Bioscience and Agriculture Research, 24(02), (2020): 2025-2029.
 
APA
Sharmin, S., Shimasaki, K. and Jamal Uddin, A. F. M.  (2020). In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs. Journal of Bioscience and Agriculture Research, 24(02), 2025-2029.
 
Chicago
Sharmin, S., Shimasaki, K. and Jamal Uddin, A. F. M. “In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs”. Journal of Bioscience and Agriculture Research, 24(01), (2020) pp. 2025-2029.
 
Harvard
Sharmin, S., Shimasaki, K. and Jamal Uddin, A. F. M. 2020. In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs. Journal of Bioscience and Agriculture Research, 24(01), 2025-2029.
 
Vancouver
Sharmin, S, Shimasaki, K and Jamal Uddin, AFM. In vitro regeneration of an Oncidium cultivar using different concentrations of BAP under different LEDs. Journal of Bioscience and Agriculture Research, 2020 May 24(01), 2025-2029.

  • Article No. 249
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Article Type: Research Article | No. 249, 2020 | Country: Bangladesh | pp. 2030-2038 | Open Access
Title: Effect of supplemental Aloe vera gel and amla fruit extract in drinking water on growth performance, immune response, haematological profiles and gut microbial load of broiler chicken
​Authors: Md. Saiful Islam, Md. Mahabbat Ali and Falguni Dadok
DOI:​ https://doi.org/10.18801/jbar.240220.249
Effect of supplemental Aloe vera gel and amla fruit extract in drinking water on growth performance, immune response, haematological profiles and gut microbial load of broiler chicken
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Abstract
The use of herbs and spices has gained increasing interest as feed additives and possible alternatives to antibiotics in poultry production. The effects of using different levels of aloe vera and amla extract on growth performance, selected blood parameters, intestinal microflora, and immune response of broiler chickens were investigated in this study. 144 commercial day-old Cobb 500 broiler chicks were randomly allotted to six treatment groups with three replicates of eight chicks each. The treatments were T1 (Control), T2 (Aloe vera, 5ml/L), T3 (Aloe vera, 10ml/L), T4 (Amla 5ml/L), T5 (Amla 10ml/L), T6 (Antibiotic 0.2g/L). The experimental treatments were administered through drinking water. All the experimental birds were provided standard husbandry conditions like light, space, ventilation and relative humidity. The result showed that the average body weight of the birds at the end of the 28 days was higher (p<0.05) in all treated groups than the control. Feed conversion ratio for both the supplemented groups together with antibiotic group was significantly improved than the control group. However, Feed intake was non-significant (p>0.05) among the treatment groups. All haematological parameters (RBC, WBC, HCT and Hb) did not show any significant (p>0.05) difference among the treatments and the values were found to be within the normal ranges. Aloe vera gel group exhibited significantly improved antibody titre (P<0.05) against NDV compared to broilers in the control and other groups. Furthermore, supplementation of aloe vera and amla significantly (P<0.05) decreased gut Escherichia coli and Salmonella spp. then the control, but did not affect the total bacterial count. Thus, the findings showed that aloe vera and amla improved performance indices and gut microflora in broiler chicks. It also induced the immune response in the studied population. Therefore, aloe vera and amla extract may potentially be used as an antibiotic alternative in poultry production.
 
Key Words: Antibiotic, Feed additive, Herbs, Poultry production and Productive performance
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Effect of supplemental Aloe vera gel and amla fruit extract in drinking water on growth performance, immune response, haematological profiles and gut microbial load of broiler chicken
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MLA
Islam et al. “Effect of supplemental Aloe vera gel and amla fruit extract in drinking water on growth performance, immune response, haematological profiles and gut microbial load of broiler chicken”. Journal of Bioscience and Agriculture Research, 24(02), (2020):2030-2038.
 
APA
Islam, M. S., Ali, M. M. and Dadok, F. (2020). Effect of supplemental Aloe vera gel and amla fruit extract in drinking water on growth performance, immune response, haematological profiles and gut microbial load of broiler chicken. Journal of Bioscience and Agriculture Research, 24(02), 2030-2038.
 
Chicago
Islam, M. S., Ali, M. M. and Dadok, F. “Effect of supplemental Aloe vera gel and amla fruit extract in drinking water on growth performance, immune response, haematological profiles and gut microbial load of broiler chicken”. Journal of Bioscience and Agriculture Research, 24(02), (2020): 2030-2038.

​Harvard

Islam, M. S., Ali, M. M. and Dadok, F. 2020. Effect of supplemental Aloe vera gel and amla fruit extract in drinking water on growth performance, immune response, haematological profiles and gut microbial load of broiler chicken. Journal of Bioscience and Agriculture Research, 24(02), pp. 2030-2038.
 
Vancouver
Islam, MS, Ali, MM and Dadok, F. Effect of supplemental Aloe vera gel and amla fruit extract in drinking water on growth performance, immune response, haematological profiles and gut microbial load of broiler chicken. Journal of Bioscience and Agriculture Research, 2020 May 24(02), 2030-2038.

  • Article No. 250
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Article Type: Research Article | No. 250, 2020 | Country: Bangladesh | pp. 2039-2046 | Open Access
Title: Quality status of farmers’ saved rice (Oryza sativa) seed in Bangladesh
​Authors: Mandal, P., Priya, M. N. A., Chowdhury, A. P., Foysal M. R. A. and Hoque, M. 
DOI:​ https://doi.org/10.18801/jbar.240220.250
Quality status of farmers’ saved rice (Oryza sativa) seed in Bangladesh
​​​​​
Abstract
Farmers’ saved seed is the principal source of rice seeds in Bangladesh. However, there is no enough information on the quality status of farmers’ saved rice seed of the country. Henceforth, this study was carried out to evaluate the qualities of farmers’ saved rice seeds across the country. The current study considered thirty (30) local and high yielding varieties of farmers’ saved rice seed and subjected to moisture content, number count, purity and germination test. In the number count test, most of the contaminated varieties were admixed with other rice varieties. In most of the varieties, the purity of seed fluctuated from 95-100%. Among all varieties, the highest purity of seed was observed in Kalijira (100%), and the lowest purity was recorded in Kaliboro (85.20%). The seed moisture content varied between 12-15%. The majority of the tested varieties had moisture content above 12%, which reflected as low germination percentage in the laboratory and field condition. The 1000-seed weight was the maximum in cv. Hariath (32.33 g) and the lowest was 9.83 g in Tulsimala. Most of the variety represented better germination in the laboratory condition in compare to field. Further, investigations are needed to ascertain the seed health to come up with a concrete reason for poor germination as well as seed quality of farmers’ saved rice seed of Bangladesh.
 
Key Words: Farmers’ saved, Rice, Seed, Quality and Germination
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Quality status of farmers’ saved rice (Oryza sativa) seed in Bangladesh
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MLA

Mandal et al. “Quality status of farmers’ saved rice (Oryza sativa) seed in Bangladesh". Journal of Bioscience and Agriculture Research, 24(02), (2020): 2039-2046.
 
APA
Mandal, P., Priya, M. N. A., Chowdhury, A. P., Foysal M. R. A. and Hoque, M. (2020). Quality status of farmers’ saved rice (Oryza sativa) seed in Bangladesh. Journal of Bioscience and Agriculture Research, 24(02), 2039-2046.
 
Chicago
Mandal, P., Priya, M. N. A., Chowdhury, A. P., Foysal M. R. A. and Hoque, M. “Quality status of farmers’ saved rice (Oryza sativa) seed in Bangladesh”. Journal of Bioscience and Agriculture Research, 24(02), (2020): 2039-2046.
 
Harvard
Mandal, P., Priya, M. N. A., Chowdhury, A. P., Foysal M. R. A. and Hoque, M. 2020. Quality status of farmers’ saved rice (Oryza sativa) seed in Bangladesh. Journal of Bioscience and Agriculture Research, 24(02), pp. 2039-2046.
 
Vancouver
Mandal P, Priya MNA, Chowdhury AP, Foysal MRA and Hoque M. Quality status of farmers’ saved rice (Oryza sativa) seed in Bangladesh. Journal of Bioscience and Agriculture Research, 2020 July 24(02), 2039-2046.​

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